No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis

Abstract Mucositis is a serious adverse effect of chemotherapeutic treatment. During intestinal mucositis, the mucosal barrier is compromised, increasing the risk of severe infections. Mucositis necessitates dose reduction or pauses in treatment, which affect the outcome of the treatment. Deleted in...

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Autores principales:	Anders B. Nexoe, Andreas A. Pedersen, Sebastian von Huth, Grith L. Sorensen, Uffe Holmskov, Ping-Ping Jiang, Sönke Detlefsen, Steffen Husby, Mathias Rathe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2008085a1e9147e48fd1bb671d0f232c
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spelling	oai:doaj.org-article:2008085a1e9147e48fd1bb671d0f232c2021-12-02T16:17:17ZNo effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis10.1038/s41598-021-94076-w2045-2322https://doaj.org/article/2008085a1e9147e48fd1bb671d0f232c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94076-whttps://doaj.org/toc/2045-2322Abstract Mucositis is a serious adverse effect of chemotherapeutic treatment. During intestinal mucositis, the mucosal barrier is compromised, increasing the risk of severe infections. Mucositis necessitates dose reduction or pauses in treatment, which affect the outcome of the treatment. Deleted in malignant brain tumors 1 (DMBT1) is a secreted scavenger protein with effects on innate immunity and epithelial regeneration. We have previously shown that jejunal DMBT1 expression is increased in piglets during chemotherapeutic treatment. We hypothesized that DMBT1 ameliorates doxorubicin-induced mucositis. Individually-caged Dmbt1 +/+ (WT) and Dmbt1 −/− (KO) female mouse littermates received intraperitoneal injections of either doxorubicin or saline. They were euthanized after three (D3) or seven days (D7). Weight loss was monitored every day, and serum citrulline levels were measured at termination. Intestinal tissue was analyzed for the expression of DMBT1 and proinflammatory cytokines (IL-1β, IL-6, and TNF). Specimens from the small intestines and colon were scored for inflammation and epithelial and mucosal architecture changes. We detected no effect of DMBT1 on weight loss, serum citrulline levels, expression of proinflammatory cytokines, or histologic damage. We detected a significant increase in crypt depth in WT mice compared to that in KO mice on D3. In conclusion, DMBT1 does not affect doxorubicin-induced mucositis in mice.Anders B. NexoeAndreas A. PedersenSebastian von HuthGrith L. SorensenUffe HolmskovPing-Ping JiangSönke DetlefsenSteffen HusbyMathias RatheNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anders B. Nexoe Andreas A. Pedersen Sebastian von Huth Grith L. Sorensen Uffe Holmskov Ping-Ping Jiang Sönke Detlefsen Steffen Husby Mathias Rathe No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
description	Abstract Mucositis is a serious adverse effect of chemotherapeutic treatment. During intestinal mucositis, the mucosal barrier is compromised, increasing the risk of severe infections. Mucositis necessitates dose reduction or pauses in treatment, which affect the outcome of the treatment. Deleted in malignant brain tumors 1 (DMBT1) is a secreted scavenger protein with effects on innate immunity and epithelial regeneration. We have previously shown that jejunal DMBT1 expression is increased in piglets during chemotherapeutic treatment. We hypothesized that DMBT1 ameliorates doxorubicin-induced mucositis. Individually-caged Dmbt1 +/+ (WT) and Dmbt1 −/− (KO) female mouse littermates received intraperitoneal injections of either doxorubicin or saline. They were euthanized after three (D3) or seven days (D7). Weight loss was monitored every day, and serum citrulline levels were measured at termination. Intestinal tissue was analyzed for the expression of DMBT1 and proinflammatory cytokines (IL-1β, IL-6, and TNF). Specimens from the small intestines and colon were scored for inflammation and epithelial and mucosal architecture changes. We detected no effect of DMBT1 on weight loss, serum citrulline levels, expression of proinflammatory cytokines, or histologic damage. We detected a significant increase in crypt depth in WT mice compared to that in KO mice on D3. In conclusion, DMBT1 does not affect doxorubicin-induced mucositis in mice.
format	article
author	Anders B. Nexoe Andreas A. Pedersen Sebastian von Huth Grith L. Sorensen Uffe Holmskov Ping-Ping Jiang Sönke Detlefsen Steffen Husby Mathias Rathe
author_facet	Anders B. Nexoe Andreas A. Pedersen Sebastian von Huth Grith L. Sorensen Uffe Holmskov Ping-Ping Jiang Sönke Detlefsen Steffen Husby Mathias Rathe
author_sort	Anders B. Nexoe
title	No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
title_short	No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
title_full	No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
title_fullStr	No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
title_full_unstemmed	No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
title_sort	no effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2008085a1e9147e48fd1bb671d0f232c
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No effect of deleted in malignant brain tumors 1 deficiency on chemotherapy induced murine intestinal mucositis

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