The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study

The contemporary theory of the inflammatory-immunological pathomechanism of atherosclerosis includes the participation of interleukin-1β (Il), Il-6, Il-10, Il-12, RANTES, and homocysteine in this process. The knowledge on the direct effect of hyperhomocysteinemia on inflammatory-state-related athero...

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Autores principales:	Magdalena Borowska, Hanna Winiarska, Marzena Dworacka, Anna Wesołowska, Grzegorz Dworacki, Przemysław Łukasz Mikołajczak
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	homocysteine Il-1β Il-6 Il-12 Il-10 RANTES Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/2009a6eb383546b1b06abd13928b3e43
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spelling	oai:doaj.org-article:2009a6eb383546b1b06abd13928b3e432021-11-11T18:37:56ZThe Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study10.3390/molecules262166711420-3049https://doaj.org/article/2009a6eb383546b1b06abd13928b3e432021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6671https://doaj.org/toc/1420-3049The contemporary theory of the inflammatory-immunological pathomechanism of atherosclerosis includes the participation of interleukin-1β (Il), Il-6, Il-10, Il-12, RANTES, and homocysteine in this process. The knowledge on the direct effect of hyperhomocysteinemia on inflammatory-state-related atherosclerosis is rather scarce. Our study is the first to account for the effects of homocysteine on the secretion of Il-10 and RANTES in vitro conditions. For this purpose, human mitogen-stimulated peripheral blood mononuclear cells (PBMNCs) were cultured in vitro and exposed to homocysteine at high concentrations. Subsequently, the concentrations of cytokines were assayed in the cell culture supernatant using flow cytofluorimetry. It has been shown that, in the presence of homocysteine, the secretion of IL-1, IL-6 and RANTES by PBMNCs was increased, whereas IL-10 concentration was significantly lower than that of the supernatant derived from a mitogen-stimulated cell culture without homocysteine. The secretion of Il-12 by PBMNCs exposed exclusively to mitogen, did not differ from homologous cells also treated with homocysteine. Therefore, in our opinion, high-concentration homocysteine affects the progression of atherosclerosis by increasing the secretion of proinflammatory cytokines secreted by PBMNCs, such as Il-1β, Il-6, RANTES, and by attenuating the secretion of Il-10.Magdalena BorowskaHanna WiniarskaMarzena DworackaAnna WesołowskaGrzegorz DworackiPrzemysław Łukasz MikołajczakMDPI AGarticlehomocysteineIl-1βIl-6Il-12Il-10RANTESOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6671, p 6671 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	homocysteine Il-1β Il-6 Il-12 Il-10 RANTES Organic chemistry QD241-441
spellingShingle	homocysteine Il-1β Il-6 Il-12 Il-10 RANTES Organic chemistry QD241-441 Magdalena Borowska Hanna Winiarska Marzena Dworacka Anna Wesołowska Grzegorz Dworacki Przemysław Łukasz Mikołajczak The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study
description	The contemporary theory of the inflammatory-immunological pathomechanism of atherosclerosis includes the participation of interleukin-1β (Il), Il-6, Il-10, Il-12, RANTES, and homocysteine in this process. The knowledge on the direct effect of hyperhomocysteinemia on inflammatory-state-related atherosclerosis is rather scarce. Our study is the first to account for the effects of homocysteine on the secretion of Il-10 and RANTES in vitro conditions. For this purpose, human mitogen-stimulated peripheral blood mononuclear cells (PBMNCs) were cultured in vitro and exposed to homocysteine at high concentrations. Subsequently, the concentrations of cytokines were assayed in the cell culture supernatant using flow cytofluorimetry. It has been shown that, in the presence of homocysteine, the secretion of IL-1, IL-6 and RANTES by PBMNCs was increased, whereas IL-10 concentration was significantly lower than that of the supernatant derived from a mitogen-stimulated cell culture without homocysteine. The secretion of Il-12 by PBMNCs exposed exclusively to mitogen, did not differ from homologous cells also treated with homocysteine. Therefore, in our opinion, high-concentration homocysteine affects the progression of atherosclerosis by increasing the secretion of proinflammatory cytokines secreted by PBMNCs, such as Il-1β, Il-6, RANTES, and by attenuating the secretion of Il-10.
format	article
author	Magdalena Borowska Hanna Winiarska Marzena Dworacka Anna Wesołowska Grzegorz Dworacki Przemysław Łukasz Mikołajczak
author_facet	Magdalena Borowska Hanna Winiarska Marzena Dworacka Anna Wesołowska Grzegorz Dworacki Przemysław Łukasz Mikołajczak
author_sort	Magdalena Borowska
title	The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study
title_short	The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study
title_full	The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study
title_fullStr	The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study
title_full_unstemmed	The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study
title_sort	effect of homocysteine on the secretion of il-1β, il-6, il-10, il-12 and rantes by peripheral blood mononuclear cells—an in vitro study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2009a6eb383546b1b06abd13928b3e43
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The Effect of Homocysteine on the Secretion of Il-1β, Il-6, Il-10, Il-12 and RANTES by Peripheral Blood Mononuclear Cells—An In Vitro Study

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