High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening

Reliable, multiplexed gene editing to uncover synergies between targets remains challenging. Here, the authors engineer AsCas12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening.

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Autores principales:	Rodrigo A. Gier, Krista A. Budinich, Niklaus H. Evitt, Zhendong Cao, Elizabeth S. Freilich, Qingzhou Chen, Jun Qi, Yemin Lan, Rahul M. Kohli, Junwei Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/200dde47acd44d708c32a6bcf129cbd0
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spelling	oai:doaj.org-article:200dde47acd44d708c32a6bcf129cbd02021-12-02T18:36:59ZHigh-performance CRISPR-Cas12a genome editing for combinatorial genetic screening10.1038/s41467-020-17209-12041-1723https://doaj.org/article/200dde47acd44d708c32a6bcf129cbd02020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17209-1https://doaj.org/toc/2041-1723Reliable, multiplexed gene editing to uncover synergies between targets remains challenging. Here, the authors engineer AsCas12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening.Rodrigo A. GierKrista A. BudinichNiklaus H. EvittZhendong CaoElizabeth S. FreilichQingzhou ChenJun QiYemin LanRahul M. KohliJunwei ShiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Rodrigo A. Gier Krista A. Budinich Niklaus H. Evitt Zhendong Cao Elizabeth S. Freilich Qingzhou Chen Jun Qi Yemin Lan Rahul M. Kohli Junwei Shi High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
description	Reliable, multiplexed gene editing to uncover synergies between targets remains challenging. Here, the authors engineer AsCas12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening.
format	article
author	Rodrigo A. Gier Krista A. Budinich Niklaus H. Evitt Zhendong Cao Elizabeth S. Freilich Qingzhou Chen Jun Qi Yemin Lan Rahul M. Kohli Junwei Shi
author_facet	Rodrigo A. Gier Krista A. Budinich Niklaus H. Evitt Zhendong Cao Elizabeth S. Freilich Qingzhou Chen Jun Qi Yemin Lan Rahul M. Kohli Junwei Shi
author_sort	Rodrigo A. Gier
title	High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
title_short	High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
title_full	High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
title_fullStr	High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
title_full_unstemmed	High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
title_sort	high-performance crispr-cas12a genome editing for combinatorial genetic screening
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/200dde47acd44d708c32a6bcf129cbd0
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High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening

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