High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening
Reliable, multiplexed gene editing to uncover synergies between targets remains challenging. Here, the authors engineer AsCas12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening.
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Autores principales: | Rodrigo A. Gier, Krista A. Budinich, Niklaus H. Evitt, Zhendong Cao, Elizabeth S. Freilich, Qingzhou Chen, Jun Qi, Yemin Lan, Rahul M. Kohli, Junwei Shi |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2020
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Materias: | |
Acceso en línea: | https://doaj.org/article/200dde47acd44d708c32a6bcf129cbd0 |
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