The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma

(1) Background: Hepatocellular carcinoma (HCC) is the most serious complication of chronic hepatitis B (CHB). Recently, the detection of circulating cell-free (cf) DNA and nucleosomes has found numerous applications in oncology. This study aimed to examine the levels of serum cfDNA markers and nucle...

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Autores principales:	Alkistis Papatheodoridi, Nikolaos Karakousis, Panagiotis Lembessis, Antonios Chatzigeorgiou, George V. Papatheodoridis
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis B hepatocellular carcinoma cell-free DNA nucleosomes DNA integrity Medicine R
Acceso en línea:	https://doaj.org/article/2012567e8d4344fa98bf9487c2004364
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spelling	oai:doaj.org-article:2012567e8d4344fa98bf9487c20043642021-11-25T18:38:56ZThe Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma10.3390/pathogens101115242076-0817https://doaj.org/article/2012567e8d4344fa98bf9487c20043642021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1524https://doaj.org/toc/2076-0817(1) Background: Hepatocellular carcinoma (HCC) is the most serious complication of chronic hepatitis B (CHB). Recently, the detection of circulating cell-free (cf) DNA and nucleosomes has found numerous applications in oncology. This study aimed to examine the levels of serum cfDNA markers and nucleosomes in CHB patients with and without HCC and assess their potential association with HCC patients’ survival. (2) Methods: Nineteen patients with CHB and HCC and 38 matched patients with CHB without cancer development during 5 years of antiviral therapy were included. Stored serum samples were analyzed for cfDNA species, including the cfDNA concentration and levels of Alu115, Alu247, and nucleosomes. DNA integrity was expressed as the Alu247/Alu115 ratio. (3) Results: Compared to controls, HCC patients had higher median Alu247 levels (64.2 vs. 23.2 genomic equivalent, <i>p</i> = 0.004) and DNA integrity (1.0 vs. 0.7, <i>p</i> < 0.001) and a trend for a higher median cfDNA concentration (36.0 vs. 19.5 ng/mL, <i>p</i> = 0.064). Increased DNA integrity (Alu247/Alu115 > 1) was associated with an increased risk of death during the first year after HCC diagnosis (<i>p</i> = 0.016). (4) Conclusions: Levels of Alu247 and DNA integrity in serum cfDNA are elevated in CHB patients with HCC, whereas increased DNA integrity seems to be associated with a worse short-term prognosis in this setting.Alkistis PapatheodoridiNikolaos KarakousisPanagiotis LembessisAntonios ChatzigeorgiouGeorge V. PapatheodoridisMDPI AGarticlehepatitis Bhepatocellular carcinomacell-free DNAnucleosomesDNA integrityMedicineRENPathogens, Vol 10, Iss 1524, p 1524 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis B hepatocellular carcinoma cell-free DNA nucleosomes DNA integrity Medicine R
spellingShingle	hepatitis B hepatocellular carcinoma cell-free DNA nucleosomes DNA integrity Medicine R Alkistis Papatheodoridi Nikolaos Karakousis Panagiotis Lembessis Antonios Chatzigeorgiou George V. Papatheodoridis The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma
description	(1) Background: Hepatocellular carcinoma (HCC) is the most serious complication of chronic hepatitis B (CHB). Recently, the detection of circulating cell-free (cf) DNA and nucleosomes has found numerous applications in oncology. This study aimed to examine the levels of serum cfDNA markers and nucleosomes in CHB patients with and without HCC and assess their potential association with HCC patients’ survival. (2) Methods: Nineteen patients with CHB and HCC and 38 matched patients with CHB without cancer development during 5 years of antiviral therapy were included. Stored serum samples were analyzed for cfDNA species, including the cfDNA concentration and levels of Alu115, Alu247, and nucleosomes. DNA integrity was expressed as the Alu247/Alu115 ratio. (3) Results: Compared to controls, HCC patients had higher median Alu247 levels (64.2 vs. 23.2 genomic equivalent, <i>p</i> = 0.004) and DNA integrity (1.0 vs. 0.7, <i>p</i> < 0.001) and a trend for a higher median cfDNA concentration (36.0 vs. 19.5 ng/mL, <i>p</i> = 0.064). Increased DNA integrity (Alu247/Alu115 > 1) was associated with an increased risk of death during the first year after HCC diagnosis (<i>p</i> = 0.016). (4) Conclusions: Levels of Alu247 and DNA integrity in serum cfDNA are elevated in CHB patients with HCC, whereas increased DNA integrity seems to be associated with a worse short-term prognosis in this setting.
format	article
author	Alkistis Papatheodoridi Nikolaos Karakousis Panagiotis Lembessis Antonios Chatzigeorgiou George V. Papatheodoridis
author_facet	Alkistis Papatheodoridi Nikolaos Karakousis Panagiotis Lembessis Antonios Chatzigeorgiou George V. Papatheodoridis
author_sort	Alkistis Papatheodoridi
title	The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma
title_short	The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma
title_full	The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma
title_fullStr	The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma
title_full_unstemmed	The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma
title_sort	significance of circulating cell-free dna markers in chronic hepatitis b patients with hepatocellular carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2012567e8d4344fa98bf9487c2004364
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The Significance of Circulating Cell-Free DNA Markers in Chronic Hepatitis B Patients with Hepatocellular Carcinoma

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