NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates

NACHT, LRR and PYD domains-containing protein 3 (NLRP3) protein is the core actor involved in inflammasome formation, and plays a pivotal role in the innate immune response. However, whether NLRP3 participates in the regulation of innate immunity in invertebrates is still unknown. In the present stu...

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Autores principales:	Zhihong Zheng, Deyi Tang, Weiling Zhao, Zhitian Wan, Mingli Yu, Ziyan Huang, Liang Li, Jude Juventus Aweya, Yueling Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Penaeus vannamei NLRP3-like Regulate Antimicrobial peptides Zoology QL1-991
Acceso en línea:	https://doaj.org/article/201832401bf24572942bd0c86ac91157
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spelling	oai:doaj.org-article:201832401bf24572942bd0c86ac911572021-11-26T04:42:33ZNLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates2667-011910.1016/j.fsirep.2021.100039https://doaj.org/article/201832401bf24572942bd0c86ac911572021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667011921000347https://doaj.org/toc/2667-0119NACHT, LRR and PYD domains-containing protein 3 (NLRP3) protein is the core actor involved in inflammasome formation, and plays a pivotal role in the innate immune response. However, whether NLRP3 participates in the regulation of innate immunity in invertebrates is still unknown. In the present study, we characterized a NLRP3 ortholog in Penaeus vannamei (designated PvNLRP3-like) with 2514 bp length of open reading frame (ORF) encoding a putative protein of 837 amino acids. Sequence analysis revealed that PvNLRP3-like contained only NACHT domain and shared closely homology with other invertebrates, but the topological structure of NACHT domain of PvNLRP3-like is similar with that in human NLRP3. PvNLRP3-like was ubiquitously expressed in tissues and induced in hemocytes by Vibrio parahaemolyticus, Streptococcus iniae and White Spot Syndrome Virus (WSSV) challenge, suggesting that PvNLRP3-like participated in the immune responses to pathogens. Furthermore, silencing of PvNLRP3-like followed by V. parahaemolyticus stimulation negatively regulated the transcripts of antimicrobial peptides (AMPs) including Lysozyme (LYZ) 3, Crustin (CRU) 2, Anti-lipopolysaccharide factor (ALF) 2/3 and Penaeidins (PEN) 3/4. This study enriches our current knowledge on shrimp innate immunity, and provides novel perspective to understand the immune regulation role of PvNLRP3-like.Zhihong ZhengDeyi TangWeiling ZhaoZhitian WanMingli YuZiyan HuangLiang LiJude Juventus AweyaYueling ZhangElsevierarticlePenaeus vannameiNLRP3-likeRegulateAntimicrobial peptidesZoologyQL1-991ENFish and Shellfish Immunology Reports, Vol 2, Iss , Pp 100039- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Penaeus vannamei NLRP3-like Regulate Antimicrobial peptides Zoology QL1-991
spellingShingle	Penaeus vannamei NLRP3-like Regulate Antimicrobial peptides Zoology QL1-991 Zhihong Zheng Deyi Tang Weiling Zhao Zhitian Wan Mingli Yu Ziyan Huang Liang Li Jude Juventus Aweya Yueling Zhang NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates
description	NACHT, LRR and PYD domains-containing protein 3 (NLRP3) protein is the core actor involved in inflammasome formation, and plays a pivotal role in the innate immune response. However, whether NLRP3 participates in the regulation of innate immunity in invertebrates is still unknown. In the present study, we characterized a NLRP3 ortholog in Penaeus vannamei (designated PvNLRP3-like) with 2514 bp length of open reading frame (ORF) encoding a putative protein of 837 amino acids. Sequence analysis revealed that PvNLRP3-like contained only NACHT domain and shared closely homology with other invertebrates, but the topological structure of NACHT domain of PvNLRP3-like is similar with that in human NLRP3. PvNLRP3-like was ubiquitously expressed in tissues and induced in hemocytes by Vibrio parahaemolyticus, Streptococcus iniae and White Spot Syndrome Virus (WSSV) challenge, suggesting that PvNLRP3-like participated in the immune responses to pathogens. Furthermore, silencing of PvNLRP3-like followed by V. parahaemolyticus stimulation negatively regulated the transcripts of antimicrobial peptides (AMPs) including Lysozyme (LYZ) 3, Crustin (CRU) 2, Anti-lipopolysaccharide factor (ALF) 2/3 and Penaeidins (PEN) 3/4. This study enriches our current knowledge on shrimp innate immunity, and provides novel perspective to understand the immune regulation role of PvNLRP3-like.
format	article
author	Zhihong Zheng Deyi Tang Weiling Zhao Zhitian Wan Mingli Yu Ziyan Huang Liang Li Jude Juventus Aweya Yueling Zhang
author_facet	Zhihong Zheng Deyi Tang Weiling Zhao Zhitian Wan Mingli Yu Ziyan Huang Liang Li Jude Juventus Aweya Yueling Zhang
author_sort	Zhihong Zheng
title	NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates
title_short	NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates
title_full	NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates
title_fullStr	NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates
title_full_unstemmed	NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates
title_sort	nlrp3-like protein negatively regulates the expression of antimicrobial peptides in penaeus vannamei hemocyates
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/201832401bf24572942bd0c86ac91157
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NLRP3-like protein negatively regulates the expression of antimicrobial peptides in Penaeus vannamei hemocyates

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