Transcriptome analysis of resistance mechanism to potato wart disease

To understand the molecular mechanism of the resistance to potato wart disease, we used the potato cultivar Qingshu 9 as the experimental material and prepared potato samples with different levels of disease through inoculation. The RNAs of the samples were extracted, and transcriptome analysis was...

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Autores principales:	Li Peihua, Fan Ruihong, Peng Zhengsong, Qing Yuan, Fang Zhirong
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	potato wart disease food safety high-throughput sequencing differentially expressed genes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/20265d00efec4310bacd5095d5dbb36c
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spelling	oai:doaj.org-article:20265d00efec4310bacd5095d5dbb36c2021-12-05T14:10:41ZTranscriptome analysis of resistance mechanism to potato wart disease2391-541210.1515/biol-2021-0045https://doaj.org/article/20265d00efec4310bacd5095d5dbb36c2021-05-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0045https://doaj.org/toc/2391-5412To understand the molecular mechanism of the resistance to potato wart disease, we used the potato cultivar Qingshu 9 as the experimental material and prepared potato samples with different levels of disease through inoculation. The RNAs of the samples were extracted, and transcriptome analysis was performed on the samples not infected by the disease (control group) and also on the samples with different levels of disease, with the aid of high-throughput sequencing. Next, the differentially expressed genes (DEGs) associated with the resistance to potato wart disease were identified based on the analysis results. Using bioinformatic tools, the DEGs were functionally annotated, classified, and enriched in related metabolic pathways. The main results are as follows: Compared with the control group, 4 DEGs were identified in the samples with light disease, 52 were found in the samples with medium disease, and 214 were discovered in the samples with heavy disease. Potato mainly resists the wart disease by suppressing its gene expression, and the degree of suppression depends on the level of the disease; the disease resistance might be dominated by cellular nucleic acid-binding protein, AP2-like transcription factor, and E3 ubiquitin-protein ligase. This research provides new insights into the molecular mechanism of potato resistance against wart disease.Li PeihuaFan RuihongPeng ZhengsongQing YuanFang ZhirongDe Gruyterarticlepotato wart diseasefood safetyhigh-throughput sequencingdifferentially expressed genesBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 475-481 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	potato wart disease food safety high-throughput sequencing differentially expressed genes Biology (General) QH301-705.5
spellingShingle	potato wart disease food safety high-throughput sequencing differentially expressed genes Biology (General) QH301-705.5 Li Peihua Fan Ruihong Peng Zhengsong Qing Yuan Fang Zhirong Transcriptome analysis of resistance mechanism to potato wart disease
description	To understand the molecular mechanism of the resistance to potato wart disease, we used the potato cultivar Qingshu 9 as the experimental material and prepared potato samples with different levels of disease through inoculation. The RNAs of the samples were extracted, and transcriptome analysis was performed on the samples not infected by the disease (control group) and also on the samples with different levels of disease, with the aid of high-throughput sequencing. Next, the differentially expressed genes (DEGs) associated with the resistance to potato wart disease were identified based on the analysis results. Using bioinformatic tools, the DEGs were functionally annotated, classified, and enriched in related metabolic pathways. The main results are as follows: Compared with the control group, 4 DEGs were identified in the samples with light disease, 52 were found in the samples with medium disease, and 214 were discovered in the samples with heavy disease. Potato mainly resists the wart disease by suppressing its gene expression, and the degree of suppression depends on the level of the disease; the disease resistance might be dominated by cellular nucleic acid-binding protein, AP2-like transcription factor, and E3 ubiquitin-protein ligase. This research provides new insights into the molecular mechanism of potato resistance against wart disease.
format	article
author	Li Peihua Fan Ruihong Peng Zhengsong Qing Yuan Fang Zhirong
author_facet	Li Peihua Fan Ruihong Peng Zhengsong Qing Yuan Fang Zhirong
author_sort	Li Peihua
title	Transcriptome analysis of resistance mechanism to potato wart disease
title_short	Transcriptome analysis of resistance mechanism to potato wart disease
title_full	Transcriptome analysis of resistance mechanism to potato wart disease
title_fullStr	Transcriptome analysis of resistance mechanism to potato wart disease
title_full_unstemmed	Transcriptome analysis of resistance mechanism to potato wart disease
title_sort	transcriptome analysis of resistance mechanism to potato wart disease
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/20265d00efec4310bacd5095d5dbb36c
work_keys_str_mv	AT lipeihua transcriptomeanalysisofresistancemechanismtopotatowartdisease AT fanruihong transcriptomeanalysisofresistancemechanismtopotatowartdisease AT pengzhengsong transcriptomeanalysisofresistancemechanismtopotatowartdisease AT qingyuan transcriptomeanalysisofresistancemechanismtopotatowartdisease AT fangzhirong transcriptomeanalysisofresistancemechanismtopotatowartdisease
_version_	1718371828237336576

Transcriptome analysis of resistance mechanism to potato wart disease

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