Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.

Wide spectrum medicinal significance augments plant utilization as the primary source of significant pharmaceutical agents. In vitro investigation of antioxidant and antimicrobial activity highlights the therapeutic potential of <i>Otostegia limbata</i>. Methanol extract of the plant (ME...

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Autores principales:	Huma Mehreen Sadaf, Yamin Bibi, Muhammad Ishaque, Sobia Nisa, Abdul Qayyum, Naila Safdar, Zahid Hussain Shah, Hameed Alsamadany, Gyuhwa Chung
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antimicrobial DPPH crude extract medicinal plants Botany QK1-989
Acceso en línea:	https://doaj.org/article/202bcbb9cb5e46f7b40d81878f1b937f
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spelling	oai:doaj.org-article:202bcbb9cb5e46f7b40d81878f1b937f2021-11-25T18:45:47ZDetermination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.10.3390/plants101123602223-7747https://doaj.org/article/202bcbb9cb5e46f7b40d81878f1b937f2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2360https://doaj.org/toc/2223-7747Wide spectrum medicinal significance augments plant utilization as the primary source of significant pharmaceutical agents. In vitro investigation of antioxidant and antimicrobial activity highlights the therapeutic potential of <i>Otostegia limbata</i>. Methanol extract of the plant (MEP) shows considerable dose dependent antioxidant ability at six concentrations (7.81 µg/mL to 250 µg/mL) in 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay, phosphomolybdate assay (PMA) and reducing power assay (RPA). The plant capability to scavenge free radicals in the mixture ranged from 37.89% to 63.50% in a concentration-dependent manner. MEP was active against five tested bacterial strains in the agar-well diffusion method. <i>Staphylococcus aureus</i>, gram-positive bacteria was found to be most susceptible followed by <i>S. epidermidis</i> with 18.80 mm and 17.47 mm mean zone of inhibition. The mean inhibition zone against gram-negative strains <i>Klebsiella pneumonia</i>, <i>Pseudomonas</i> spp. and <i>Escherichia coli</i> were 15.07 mm, 14.73 mm, and 12.17 mm. MEP revealed potential against <i>Alternaria</i> spp. and <i>Aspergillus terreus</i> fungal strains evaluated through agar-tube dilution assay. <i>Aspergillus terreus</i> was more sensitive than <i>Alternaria</i> spp. with an average 78.45% and 68.0% inhibition. These findings can serve as a benchmark for forthcoming scrutiny such as bioactive components discovery and drug development.Huma Mehreen SadafYamin BibiMuhammad IshaqueSobia NisaAbdul QayyumNaila SafdarZahid Hussain ShahHameed AlsamadanyGyuhwa ChungMDPI AGarticleantimicrobialDPPHcrude extractmedicinal plantsBotanyQK1-989ENPlants, Vol 10, Iss 2360, p 2360 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial DPPH crude extract medicinal plants Botany QK1-989
spellingShingle	antimicrobial DPPH crude extract medicinal plants Botany QK1-989 Huma Mehreen Sadaf Yamin Bibi Muhammad Ishaque Sobia Nisa Abdul Qayyum Naila Safdar Zahid Hussain Shah Hameed Alsamadany Gyuhwa Chung Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.
description	Wide spectrum medicinal significance augments plant utilization as the primary source of significant pharmaceutical agents. In vitro investigation of antioxidant and antimicrobial activity highlights the therapeutic potential of <i>Otostegia limbata</i>. Methanol extract of the plant (MEP) shows considerable dose dependent antioxidant ability at six concentrations (7.81 µg/mL to 250 µg/mL) in 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay, phosphomolybdate assay (PMA) and reducing power assay (RPA). The plant capability to scavenge free radicals in the mixture ranged from 37.89% to 63.50% in a concentration-dependent manner. MEP was active against five tested bacterial strains in the agar-well diffusion method. <i>Staphylococcus aureus</i>, gram-positive bacteria was found to be most susceptible followed by <i>S. epidermidis</i> with 18.80 mm and 17.47 mm mean zone of inhibition. The mean inhibition zone against gram-negative strains <i>Klebsiella pneumonia</i>, <i>Pseudomonas</i> spp. and <i>Escherichia coli</i> were 15.07 mm, 14.73 mm, and 12.17 mm. MEP revealed potential against <i>Alternaria</i> spp. and <i>Aspergillus terreus</i> fungal strains evaluated through agar-tube dilution assay. <i>Aspergillus terreus</i> was more sensitive than <i>Alternaria</i> spp. with an average 78.45% and 68.0% inhibition. These findings can serve as a benchmark for forthcoming scrutiny such as bioactive components discovery and drug development.
format	article
author	Huma Mehreen Sadaf Yamin Bibi Muhammad Ishaque Sobia Nisa Abdul Qayyum Naila Safdar Zahid Hussain Shah Hameed Alsamadany Gyuhwa Chung
author_facet	Huma Mehreen Sadaf Yamin Bibi Muhammad Ishaque Sobia Nisa Abdul Qayyum Naila Safdar Zahid Hussain Shah Hameed Alsamadany Gyuhwa Chung
author_sort	Huma Mehreen Sadaf
title	Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.
title_short	Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.
title_full	Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.
title_fullStr	Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.
title_full_unstemmed	Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.
title_sort	determination of ros scavenging, antibacterial and antifungal potential of methanolic extract of <i>otostegia limbata</i> (benth.) boiss.
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/202bcbb9cb5e46f7b40d81878f1b937f
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Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of <i>Otostegia limbata</i> (Benth.) Boiss.

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