First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam

Abstract Background Entomopathogenic nematodes (EPNs) are evidently a useful nematode group for the biocontrol of insect pests. It is well known that efficacy of different EPN strains, even belonging to the same species, can be significantly varied in different localities. Therefore, exploring EPNs...

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Autores principales:	Phap Quang Trinh, Duyen Thi Nguyen, Linh Thi Mai Le, Tien Huu Nguyen
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Entomopathogenic nematodes Steinernema surkhetense Lethal efficacy Galleria mellonella Central Highlands, Vietnam Biocontrol Agriculture S
Acceso en línea:	https://doaj.org/article/2030f3b02ac54d33af81d9c27868aea8
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spelling	oai:doaj.org-article:2030f3b02ac54d33af81d9c27868aea82021-11-28T12:06:17ZFirst report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam10.1186/s41938-021-00496-12536-9342https://doaj.org/article/2030f3b02ac54d33af81d9c27868aea82021-11-01T00:00:00Zhttps://doi.org/10.1186/s41938-021-00496-1https://doaj.org/toc/2536-9342Abstract Background Entomopathogenic nematodes (EPNs) are evidently a useful nematode group for the biocontrol of insect pests. It is well known that efficacy of different EPN strains, even belonging to the same species, can be significantly varied in different localities. Therefore, exploring EPNs and testing their efficacy in various ecological regions is of crucial importance to find out more efficient EPN strains. On the other hand, this practice is also needed to enhance the knowledge on diversity and distribution model of EPNs over the world. Results In this study, a species belonging to the genus Steinernema, S. surkhetense, has been characterized for the first time in Vietnam based on morphological and molecular characterizations. Morphological characterizations of infective juveniles, the first and second-generation adults, and molecular characterization of D2-D3 expansion segment of 28S rRNA region were given. Molecular phylogeny of the genus Steinernema was also provided. In addition, the study showed that the lethal efficacy of this local strain to larvae of Galleria mellonella L. was relatively higher than other reported EPN strains in Vietnam. Conclusions The Vietnamese EPN population found in this study was determined to be conspecific with S. surkhetense, revealed its new distribution in Vietnam. Besides, detailed morphological and molecular characterizations of it was provided with small variations compared to other populations in the world, and its relatively high lethal efficacy on larvae of G. mellonella implied that this strain can be potentially a useful strain for biological control of insect pests.Phap Quang TrinhDuyen Thi NguyenLinh Thi Mai LeTien Huu NguyenSpringerOpenarticleEntomopathogenic nematodesSteinernema surkhetenseLethal efficacyGalleria mellonellaCentral Highlands, VietnamBiocontrolAgricultureSENEgyptian Journal of Biological Pest Control, Vol 31, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Entomopathogenic nematodes Steinernema surkhetense Lethal efficacy Galleria mellonella Central Highlands, Vietnam Biocontrol Agriculture S
spellingShingle	Entomopathogenic nematodes Steinernema surkhetense Lethal efficacy Galleria mellonella Central Highlands, Vietnam Biocontrol Agriculture S Phap Quang Trinh Duyen Thi Nguyen Linh Thi Mai Le Tien Huu Nguyen First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
description	Abstract Background Entomopathogenic nematodes (EPNs) are evidently a useful nematode group for the biocontrol of insect pests. It is well known that efficacy of different EPN strains, even belonging to the same species, can be significantly varied in different localities. Therefore, exploring EPNs and testing their efficacy in various ecological regions is of crucial importance to find out more efficient EPN strains. On the other hand, this practice is also needed to enhance the knowledge on diversity and distribution model of EPNs over the world. Results In this study, a species belonging to the genus Steinernema, S. surkhetense, has been characterized for the first time in Vietnam based on morphological and molecular characterizations. Morphological characterizations of infective juveniles, the first and second-generation adults, and molecular characterization of D2-D3 expansion segment of 28S rRNA region were given. Molecular phylogeny of the genus Steinernema was also provided. In addition, the study showed that the lethal efficacy of this local strain to larvae of Galleria mellonella L. was relatively higher than other reported EPN strains in Vietnam. Conclusions The Vietnamese EPN population found in this study was determined to be conspecific with S. surkhetense, revealed its new distribution in Vietnam. Besides, detailed morphological and molecular characterizations of it was provided with small variations compared to other populations in the world, and its relatively high lethal efficacy on larvae of G. mellonella implied that this strain can be potentially a useful strain for biological control of insect pests.
format	article
author	Phap Quang Trinh Duyen Thi Nguyen Linh Thi Mai Le Tien Huu Nguyen
author_facet	Phap Quang Trinh Duyen Thi Nguyen Linh Thi Mai Le Tien Huu Nguyen
author_sort	Phap Quang Trinh
title	First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
title_short	First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
title_full	First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
title_fullStr	First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
title_full_unstemmed	First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam
title_sort	first report of entomopathogenic nematode steinernema surkhetense and its pathogenic potential to larvae of the greater wax moth (galleria mellonella l.) in vietnam
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/2030f3b02ac54d33af81d9c27868aea8
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First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam

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