Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice

Abstract Many studies have shown that epigallocatechin gallate (EGCg) contribute to the health benefits of green tea, although its bioavailability is usually low. However, the mechanism underlying its intestinal absorption remains unclear. In human subjects, it has been reported that the bioavailabi...

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Autores principales:	Shunsuke Ishii, Hidefumi Kitazawa, Takuya Mori, Aya Kirino, Shun Nakamura, Noriko Osaki, Akira Shimotoyodome, Ikumi Tamai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20310856ad67498ba486bef6223697a1
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spelling	oai:doaj.org-article:20310856ad67498ba486bef6223697a12021-12-02T15:09:56ZIdentification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice10.1038/s41598-019-47214-42045-2322https://doaj.org/article/20310856ad67498ba486bef6223697a12019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47214-4https://doaj.org/toc/2045-2322Abstract Many studies have shown that epigallocatechin gallate (EGCg) contribute to the health benefits of green tea, although its bioavailability is usually low. However, the mechanism underlying its intestinal absorption remains unclear. In human subjects, it has been reported that the bioavailability of EGCg increases after repeated oral catechin intake. We hypothesized that a certain uptake transporter was involved in this increase, and investigated a novel EGCg transporter. We first confirmed the increase in EGCg bioavailability in mice fed the catechin diet for two weeks. Then, in situ intestinal catechin infusion exhibited that the absorption of EGCg in the ileum was selectively increased in mice fed the catechin diet. A comprehensive analysis of plasma membrane proteins revealed 10 candidates for EGCg transporter, which were selectively increased in the ileum. EGCg uptake by a Xenopus laevis oocyte expressed with respective transporter revealed that oocytes microinjected with DTDST cRNA exhibited significantly higher EGCg uptake. Furthermore, uptake of EGCg by CHO-K1 cells stably expressing DTDST was significantly higher than that by mock cells, which was nullified by treating with a DTDST inhibitor. In conclusion, this study identified DTDST as a novel intestinal EGCg transporter that is upregulated after repeated oral catechin intake.Shunsuke IshiiHidefumi KitazawaTakuya MoriAya KirinoShun NakamuraNoriko OsakiAkira ShimotoyodomeIkumi TamaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shunsuke Ishii Hidefumi Kitazawa Takuya Mori Aya Kirino Shun Nakamura Noriko Osaki Akira Shimotoyodome Ikumi Tamai Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice
description	Abstract Many studies have shown that epigallocatechin gallate (EGCg) contribute to the health benefits of green tea, although its bioavailability is usually low. However, the mechanism underlying its intestinal absorption remains unclear. In human subjects, it has been reported that the bioavailability of EGCg increases after repeated oral catechin intake. We hypothesized that a certain uptake transporter was involved in this increase, and investigated a novel EGCg transporter. We first confirmed the increase in EGCg bioavailability in mice fed the catechin diet for two weeks. Then, in situ intestinal catechin infusion exhibited that the absorption of EGCg in the ileum was selectively increased in mice fed the catechin diet. A comprehensive analysis of plasma membrane proteins revealed 10 candidates for EGCg transporter, which were selectively increased in the ileum. EGCg uptake by a Xenopus laevis oocyte expressed with respective transporter revealed that oocytes microinjected with DTDST cRNA exhibited significantly higher EGCg uptake. Furthermore, uptake of EGCg by CHO-K1 cells stably expressing DTDST was significantly higher than that by mock cells, which was nullified by treating with a DTDST inhibitor. In conclusion, this study identified DTDST as a novel intestinal EGCg transporter that is upregulated after repeated oral catechin intake.
format	article
author	Shunsuke Ishii Hidefumi Kitazawa Takuya Mori Aya Kirino Shun Nakamura Noriko Osaki Akira Shimotoyodome Ikumi Tamai
author_facet	Shunsuke Ishii Hidefumi Kitazawa Takuya Mori Aya Kirino Shun Nakamura Noriko Osaki Akira Shimotoyodome Ikumi Tamai
author_sort	Shunsuke Ishii
title	Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice
title_short	Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice
title_full	Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice
title_fullStr	Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice
title_full_unstemmed	Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice
title_sort	identification of the catechin uptake transporter responsible for intestinal absorption of epigallocatechin gallate in mice
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/20310856ad67498ba486bef6223697a1
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Identification of the Catechin Uptake Transporter Responsible for Intestinal Absorption of Epigallocatechin Gallate in Mice

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