Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells

Abstract Mesenchymal stem cells (MSC) are osteoblasts progenitors and a variety of studies suggest that they may play an important role for the health in the field of bone regeneration. Magnesium supplementation is gaining importance as adjuvant treatment to improve osteogenesis, although the mechan...

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Autores principales:	Juan M. Díaz-Tocados, Carmen Herencia, Julio M. Martínez-Moreno, Addy Montes de Oca, Maria E. Rodríguez-Ortiz, Noemi Vergara, Alfonso Blanco, Sonja Steppan, Yolanda Almadén, Mariano Rodríguez, Juan R. Muñoz-Castañeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2035b598fbd343d99f54a64147d29151
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spelling	oai:doaj.org-article:2035b598fbd343d99f54a64147d291512021-12-02T16:06:23ZMagnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells10.1038/s41598-017-08379-y2045-2322https://doaj.org/article/2035b598fbd343d99f54a64147d291512017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08379-yhttps://doaj.org/toc/2045-2322Abstract Mesenchymal stem cells (MSC) are osteoblasts progenitors and a variety of studies suggest that they may play an important role for the health in the field of bone regeneration. Magnesium supplementation is gaining importance as adjuvant treatment to improve osteogenesis, although the mechanisms involving this process are not well understood. The objective of this study was to investigate the effects of magnesium on MSC differentiation. Here we show that in rat bone marrow MSC, magnesium chloride increases MSC proliferation in a dose-dependent manner promoting osteogenic differentiation and mineralization. These effects are reduced by 2-APB administration, an inhibitor of magnesium channel TRPM7. Of note, magnesium supplementation did not increase the canonical Wnt/β-catenin pathway, although it promoted the activation of Notch1 signaling, which was also decreased by addition of 2-APB. Electron microscopy showed higher proliferation, organization and maturation of osteoblasts in bone decellularized scaffolds after magnesium addition. In summary, our results demonstrate that magnesium chloride enhances MSC proliferation by Notch1 signaling activation and induces osteogenic differentiation, shedding light on the understanding of the role of magnesium during bone regeneration.Juan M. Díaz-TocadosCarmen HerenciaJulio M. Martínez-MorenoAddy Montes de OcaMaria E. Rodríguez-OrtizNoemi VergaraAlfonso BlancoSonja SteppanYolanda AlmadénMariano RodríguezJuan R. Muñoz-CastañedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan M. Díaz-Tocados Carmen Herencia Julio M. Martínez-Moreno Addy Montes de Oca Maria E. Rodríguez-Ortiz Noemi Vergara Alfonso Blanco Sonja Steppan Yolanda Almadén Mariano Rodríguez Juan R. Muñoz-Castañeda Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells
description	Abstract Mesenchymal stem cells (MSC) are osteoblasts progenitors and a variety of studies suggest that they may play an important role for the health in the field of bone regeneration. Magnesium supplementation is gaining importance as adjuvant treatment to improve osteogenesis, although the mechanisms involving this process are not well understood. The objective of this study was to investigate the effects of magnesium on MSC differentiation. Here we show that in rat bone marrow MSC, magnesium chloride increases MSC proliferation in a dose-dependent manner promoting osteogenic differentiation and mineralization. These effects are reduced by 2-APB administration, an inhibitor of magnesium channel TRPM7. Of note, magnesium supplementation did not increase the canonical Wnt/β-catenin pathway, although it promoted the activation of Notch1 signaling, which was also decreased by addition of 2-APB. Electron microscopy showed higher proliferation, organization and maturation of osteoblasts in bone decellularized scaffolds after magnesium addition. In summary, our results demonstrate that magnesium chloride enhances MSC proliferation by Notch1 signaling activation and induces osteogenic differentiation, shedding light on the understanding of the role of magnesium during bone regeneration.
format	article
author	Juan M. Díaz-Tocados Carmen Herencia Julio M. Martínez-Moreno Addy Montes de Oca Maria E. Rodríguez-Ortiz Noemi Vergara Alfonso Blanco Sonja Steppan Yolanda Almadén Mariano Rodríguez Juan R. Muñoz-Castañeda
author_facet	Juan M. Díaz-Tocados Carmen Herencia Julio M. Martínez-Moreno Addy Montes de Oca Maria E. Rodríguez-Ortiz Noemi Vergara Alfonso Blanco Sonja Steppan Yolanda Almadén Mariano Rodríguez Juan R. Muñoz-Castañeda
author_sort	Juan M. Díaz-Tocados
title	Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells
title_short	Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells
title_full	Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells
title_fullStr	Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells
title_full_unstemmed	Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells
title_sort	magnesium chloride promotes osteogenesis through notch signaling activation and expansion of mesenchymal stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2035b598fbd343d99f54a64147d29151
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Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells

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