Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification

Abstract Although culture-based identification of bacteria is the gold-standard for the diagnosis of infectious diseases, it is time consuming. Recent advances in molecular diagnostics and microfluidic technologies have opened up new avenues for rapid detection of bacteria. Here, we describe a centr...

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Autores principales:	He Yan, Yunzeng Zhu, Yan Zhang, Lei Wang, Junge Chen, Ying Lu, Youchun Xu, Wanli Xing
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/203e17ecc3e74772baf920e53760f312
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spelling	oai:doaj.org-article:203e17ecc3e74772baf920e53760f3122021-12-02T12:32:45ZMultiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification10.1038/s41598-017-01415-x2045-2322https://doaj.org/article/203e17ecc3e74772baf920e53760f3122017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01415-xhttps://doaj.org/toc/2045-2322Abstract Although culture-based identification of bacteria is the gold-standard for the diagnosis of infectious diseases, it is time consuming. Recent advances in molecular diagnostics and microfluidic technologies have opened up new avenues for rapid detection of bacteria. Here, we describe a centrifugal-microfluidic chip for the detection of bacteria by integrating the cell lysis, clarification, and loop-mediated amplification (LAMP). The major advantages of this chip are as follows. Firstly, bacteria lysis was innovatively achieved by rotating a pair of magnets to generate bead-beating while the chip was kept stationary during lysis, which simplified the chip design because no additional valve was needed. Secondly, the on-chip assay time was short (within 70 min), which was competitive in emergency situations. Thirdly, results of the analysis can be interpreted by using a fluorescence detector or by the naked-eye, making it versatile in many areas, especially the resource-limited areas. The on-chip limits of detection of six types of bacteria were valued by gel electrophoresis, showing the similar results compared to the bench-top LAMP protocol. This chip can be used for rapid, sensitive, accurate and automated detection of bacteria, offering a promising alternative for simplifying the molecular diagnostics of infectious diseases.He YanYunzeng ZhuYan ZhangLei WangJunge ChenYing LuYouchun XuWanli XingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q He Yan Yunzeng Zhu Yan Zhang Lei Wang Junge Chen Ying Lu Youchun Xu Wanli Xing Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
description	Abstract Although culture-based identification of bacteria is the gold-standard for the diagnosis of infectious diseases, it is time consuming. Recent advances in molecular diagnostics and microfluidic technologies have opened up new avenues for rapid detection of bacteria. Here, we describe a centrifugal-microfluidic chip for the detection of bacteria by integrating the cell lysis, clarification, and loop-mediated amplification (LAMP). The major advantages of this chip are as follows. Firstly, bacteria lysis was innovatively achieved by rotating a pair of magnets to generate bead-beating while the chip was kept stationary during lysis, which simplified the chip design because no additional valve was needed. Secondly, the on-chip assay time was short (within 70 min), which was competitive in emergency situations. Thirdly, results of the analysis can be interpreted by using a fluorescence detector or by the naked-eye, making it versatile in many areas, especially the resource-limited areas. The on-chip limits of detection of six types of bacteria were valued by gel electrophoresis, showing the similar results compared to the bench-top LAMP protocol. This chip can be used for rapid, sensitive, accurate and automated detection of bacteria, offering a promising alternative for simplifying the molecular diagnostics of infectious diseases.
format	article
author	He Yan Yunzeng Zhu Yan Zhang Lei Wang Junge Chen Ying Lu Youchun Xu Wanli Xing
author_facet	He Yan Yunzeng Zhu Yan Zhang Lei Wang Junge Chen Ying Lu Youchun Xu Wanli Xing
author_sort	He Yan
title	Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
title_short	Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
title_full	Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
title_fullStr	Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
title_full_unstemmed	Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
title_sort	multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/203e17ecc3e74772baf920e53760f312
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Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification

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