Bacterial membrane vesicles transport their DNA cargo into host cells

Abstract Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host ce...

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Autores principales:	Natalie J. Bitto, Ross Chapman, Sacha Pidot, Adam Costin, Camden Lo, Jasmine Choi, Tanya D’Cruze, Eric C. Reynolds, Stuart G. Dashper, Lynne Turnbull, Cynthia B. Whitchurch, Timothy P. Stinear, Katryn J. Stacey, Richard L. Ferrero
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/203ee8a1241a457c94f0d0e86ad5a934
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spelling	oai:doaj.org-article:203ee8a1241a457c94f0d0e86ad5a9342021-12-02T11:41:22ZBacterial membrane vesicles transport their DNA cargo into host cells10.1038/s41598-017-07288-42045-2322https://doaj.org/article/203ee8a1241a457c94f0d0e86ad5a9342017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07288-4https://doaj.org/toc/2045-2322Abstract Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism. Furthermore, we demonstrated that OMVs carry DNA into eukaryotic cells, and this DNA was detectable by PCR in the nuclear fraction of cells. These findings suggest a role for OMV-associated DNA in bacterial-host cell interactions and have implications for OMV-based vaccines.Natalie J. BittoRoss ChapmanSacha PidotAdam CostinCamden LoJasmine ChoiTanya D’CruzeEric C. ReynoldsStuart G. DashperLynne TurnbullCynthia B. WhitchurchTimothy P. StinearKatryn J. StaceyRichard L. FerreroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalie J. Bitto Ross Chapman Sacha Pidot Adam Costin Camden Lo Jasmine Choi Tanya D’Cruze Eric C. Reynolds Stuart G. Dashper Lynne Turnbull Cynthia B. Whitchurch Timothy P. Stinear Katryn J. Stacey Richard L. Ferrero Bacterial membrane vesicles transport their DNA cargo into host cells
description	Abstract Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism. Furthermore, we demonstrated that OMVs carry DNA into eukaryotic cells, and this DNA was detectable by PCR in the nuclear fraction of cells. These findings suggest a role for OMV-associated DNA in bacterial-host cell interactions and have implications for OMV-based vaccines.
format	article
author	Natalie J. Bitto Ross Chapman Sacha Pidot Adam Costin Camden Lo Jasmine Choi Tanya D’Cruze Eric C. Reynolds Stuart G. Dashper Lynne Turnbull Cynthia B. Whitchurch Timothy P. Stinear Katryn J. Stacey Richard L. Ferrero
author_facet	Natalie J. Bitto Ross Chapman Sacha Pidot Adam Costin Camden Lo Jasmine Choi Tanya D’Cruze Eric C. Reynolds Stuart G. Dashper Lynne Turnbull Cynthia B. Whitchurch Timothy P. Stinear Katryn J. Stacey Richard L. Ferrero
author_sort	Natalie J. Bitto
title	Bacterial membrane vesicles transport their DNA cargo into host cells
title_short	Bacterial membrane vesicles transport their DNA cargo into host cells
title_full	Bacterial membrane vesicles transport their DNA cargo into host cells
title_fullStr	Bacterial membrane vesicles transport their DNA cargo into host cells
title_full_unstemmed	Bacterial membrane vesicles transport their DNA cargo into host cells
title_sort	bacterial membrane vesicles transport their dna cargo into host cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/203ee8a1241a457c94f0d0e86ad5a934
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Bacterial membrane vesicles transport their DNA cargo into host cells

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