Figla promotes secondary follicle growth in mature mice

Abstract The in vitro growth (IVG) of human follicles is a potential fertility option for women for whom cryopreserved ovarian tissues cannot be transplanted due to the risk of cancer cell reintroduction; however, there is currently no established method. Furthermore, optimal IVG conditions may diff...

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Autores principales:	Asuka Okunomiya, Akihito Horie, Hirohiko Tani, Yukiyasu Sato, Shiro Takamatsu, J. B. Brown, Miki Sugimoto, Junzo Hamanishi, Eiji Kondoh, Noriomi Matsumura, Masaki Mandai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/203fc67e70f04c57a9cec91aeea62a71
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spelling	oai:doaj.org-article:203fc67e70f04c57a9cec91aeea62a712021-12-02T15:36:31ZFigla promotes secondary follicle growth in mature mice10.1038/s41598-021-89052-32045-2322https://doaj.org/article/203fc67e70f04c57a9cec91aeea62a712021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89052-3https://doaj.org/toc/2045-2322Abstract The in vitro growth (IVG) of human follicles is a potential fertility option for women for whom cryopreserved ovarian tissues cannot be transplanted due to the risk of cancer cell reintroduction; however, there is currently no established method. Furthermore, optimal IVG conditions may differ between the follicles of adult and pre-pubertal females due to molecular differences suggested by basic research. To systematically identify differences between the secondary follicles of adult and pre-pubertal females, a comparative transcriptomic study using mice was conducted herein. Among differentially expressed genes (DEGs), Figla was up-regulated in mature mice. We successfully down-regulated Figla expression in secondary follicle oocytes by a Figla siRNA microinjection, and the subsequent IVG of follicles showed that the diameter of these follicles was smaller than those of controls in mature mice, whereas no significant difference was observed in premature mice. The canonical pathways of DEGs between control and Figla-reduced secondary follicles suggest that Figla up-regulates VDR/RXR activation and down-regulates stem cell pluripotency as well as estrogen signaling. We demonstrated for the first time that folliculogenesis of the secondary follicles of premature and mature mice may be regulated by different factors, such as Figla with its possible target genes, providing insights into optimal IVG conditions for adult and pre-pubertal females, respectively.Asuka OkunomiyaAkihito HorieHirohiko TaniYukiyasu SatoShiro TakamatsuJ. B. BrownMiki SugimotoJunzo HamanishiEiji KondohNoriomi MatsumuraMasaki MandaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Asuka Okunomiya Akihito Horie Hirohiko Tani Yukiyasu Sato Shiro Takamatsu J. B. Brown Miki Sugimoto Junzo Hamanishi Eiji Kondoh Noriomi Matsumura Masaki Mandai Figla promotes secondary follicle growth in mature mice
description	Abstract The in vitro growth (IVG) of human follicles is a potential fertility option for women for whom cryopreserved ovarian tissues cannot be transplanted due to the risk of cancer cell reintroduction; however, there is currently no established method. Furthermore, optimal IVG conditions may differ between the follicles of adult and pre-pubertal females due to molecular differences suggested by basic research. To systematically identify differences between the secondary follicles of adult and pre-pubertal females, a comparative transcriptomic study using mice was conducted herein. Among differentially expressed genes (DEGs), Figla was up-regulated in mature mice. We successfully down-regulated Figla expression in secondary follicle oocytes by a Figla siRNA microinjection, and the subsequent IVG of follicles showed that the diameter of these follicles was smaller than those of controls in mature mice, whereas no significant difference was observed in premature mice. The canonical pathways of DEGs between control and Figla-reduced secondary follicles suggest that Figla up-regulates VDR/RXR activation and down-regulates stem cell pluripotency as well as estrogen signaling. We demonstrated for the first time that folliculogenesis of the secondary follicles of premature and mature mice may be regulated by different factors, such as Figla with its possible target genes, providing insights into optimal IVG conditions for adult and pre-pubertal females, respectively.
format	article
author	Asuka Okunomiya Akihito Horie Hirohiko Tani Yukiyasu Sato Shiro Takamatsu J. B. Brown Miki Sugimoto Junzo Hamanishi Eiji Kondoh Noriomi Matsumura Masaki Mandai
author_facet	Asuka Okunomiya Akihito Horie Hirohiko Tani Yukiyasu Sato Shiro Takamatsu J. B. Brown Miki Sugimoto Junzo Hamanishi Eiji Kondoh Noriomi Matsumura Masaki Mandai
author_sort	Asuka Okunomiya
title	Figla promotes secondary follicle growth in mature mice
title_short	Figla promotes secondary follicle growth in mature mice
title_full	Figla promotes secondary follicle growth in mature mice
title_fullStr	Figla promotes secondary follicle growth in mature mice
title_full_unstemmed	Figla promotes secondary follicle growth in mature mice
title_sort	figla promotes secondary follicle growth in mature mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/203fc67e70f04c57a9cec91aeea62a71
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Figla promotes secondary follicle growth in mature mice

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