Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research

Abstract For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study co...

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Autores principales:	Gil Lola Oreff, Michele Fenu, Claus Vogl, Iris Ribitsch, Florien Jenner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/204516fe7aad4dafa1165f91f4737a09
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spelling	oai:doaj.org-article:204516fe7aad4dafa1165f91f4737a092021-12-02T17:23:03ZSpecies variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research10.1038/s41598-021-91914-92045-2322https://doaj.org/article/204516fe7aad4dafa1165f91f4737a092021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91914-9https://doaj.org/toc/2045-2322Abstract For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study compared the cellular and molecular features of inflammation in tenocytes of humans and four common model species (mouse, rat, sheep, and horse). While mouse and rat tenocytes most closely equalled human tenocytes’ low proliferation capacity and the negligible effect of inflammation on proliferation, the wound closure speed of humans was best approximated by rats and horses. The overall gene expression of human tenocytes was most similar to mice under healthy, to horses under transient and to sheep under constant inflammatory conditions. Humans were best matched by mice and horses in their tendon marker and collagen expression, by horses in extracellular matrix remodelling genes, and by rats in inflammatory mediators. As no single animal model perfectly replicates the clinical condition and sufficiently emulates human tenocytes, fit-for-purpose selection of the model species for each specific research question and combination of data from multiple species will be essential to optimize translational predictive validity.Gil Lola OreffMichele FenuClaus VoglIris RibitschFlorien JennerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gil Lola Oreff Michele Fenu Claus Vogl Iris Ribitsch Florien Jenner Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
description	Abstract For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study compared the cellular and molecular features of inflammation in tenocytes of humans and four common model species (mouse, rat, sheep, and horse). While mouse and rat tenocytes most closely equalled human tenocytes’ low proliferation capacity and the negligible effect of inflammation on proliferation, the wound closure speed of humans was best approximated by rats and horses. The overall gene expression of human tenocytes was most similar to mice under healthy, to horses under transient and to sheep under constant inflammatory conditions. Humans were best matched by mice and horses in their tendon marker and collagen expression, by horses in extracellular matrix remodelling genes, and by rats in inflammatory mediators. As no single animal model perfectly replicates the clinical condition and sufficiently emulates human tenocytes, fit-for-purpose selection of the model species for each specific research question and combination of data from multiple species will be essential to optimize translational predictive validity.
format	article
author	Gil Lola Oreff Michele Fenu Claus Vogl Iris Ribitsch Florien Jenner
author_facet	Gil Lola Oreff Michele Fenu Claus Vogl Iris Ribitsch Florien Jenner
author_sort	Gil Lola Oreff
title	Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
title_short	Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
title_full	Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
title_fullStr	Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
title_full_unstemmed	Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
title_sort	species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/204516fe7aad4dafa1165f91f4737a09
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Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research

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