From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.

Pseudomonas aeruginosa is a highly versatile opportunistic pathogen capable of colonizing multiple ecological niches. This bacterium is responsible for a wide range of both acute and chronic infections in a variety of hosts. The success of this microorganism relies on its ability to adapt to environ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariette Barbier, F Heath Damron, Piotr Bielecki, María Suárez-Diez, Jacek Puchałka, Sebastian Albertí, Vitor Martins Dos Santos, Joanna B Goldberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2049a7568cc14ca88a50993dc5a3d56b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2049a7568cc14ca88a50993dc5a3d56b
record_format	dspace
spelling	oai:doaj.org-article:2049a7568cc14ca88a50993dc5a3d56b2021-11-18T08:31:13ZFrom the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.1932-620310.1371/journal.pone.0089941https://doaj.org/article/2049a7568cc14ca88a50993dc5a3d56b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587139/?tool=EBIhttps://doaj.org/toc/1932-6203Pseudomonas aeruginosa is a highly versatile opportunistic pathogen capable of colonizing multiple ecological niches. This bacterium is responsible for a wide range of both acute and chronic infections in a variety of hosts. The success of this microorganism relies on its ability to adapt to environmental changes and re-program its regulatory and metabolic networks. The study of P. aeruginosa adaptation to temperature is crucial to understanding the pathogenesis upon infection of its mammalian host. We examined the effects of growth temperature on the transcriptome of the P. aeruginosa PAO1. Microarray analysis of PAO1 grown in Lysogeny broth at mid-exponential phase at 22°C and 37°C revealed that temperature changes are responsible for the differential transcriptional regulation of 6.4% of the genome. Major alterations were observed in bacterial metabolism, replication, and nutrient acquisition. Quorum-sensing and exoproteins secreted by type I, II, and III secretion systems, involved in the adaptation of P. aeruginosa to the mammalian host during infection, were up-regulated at 37°C compared to 22°C. Genes encoding arginine degradation enzymes were highly up-regulated at 22°C, together with the genes involved in the synthesis of pyoverdine. However, genes involved in pyochelin biosynthesis were up-regulated at 37°C. We observed that the changes in expression of P. aeruginosa siderophores correlated to an overall increase in Fe²⁺ extracellular concentration at 37°C and a peak in Fe³⁺ extracellular concentration at 22°C. This suggests a distinct change in iron acquisition strategies when the bacterium switches from the external environment to the host. Our work identifies global changes in bacterial metabolism and nutrient acquisition induced by growth at different temperatures. Overall, this study identifies factors that are regulated in genome-wide adaptation processes and discusses how this life-threatening pathogen responds to temperature.Mariette BarbierF Heath DamronPiotr BieleckiMaría Suárez-DiezJacek PuchałkaSebastian AlbertíVitor Martins Dos SantosJoanna B GoldbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89941 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariette Barbier F Heath Damron Piotr Bielecki María Suárez-Diez Jacek Puchałka Sebastian Albertí Vitor Martins Dos Santos Joanna B Goldberg From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.
description	Pseudomonas aeruginosa is a highly versatile opportunistic pathogen capable of colonizing multiple ecological niches. This bacterium is responsible for a wide range of both acute and chronic infections in a variety of hosts. The success of this microorganism relies on its ability to adapt to environmental changes and re-program its regulatory and metabolic networks. The study of P. aeruginosa adaptation to temperature is crucial to understanding the pathogenesis upon infection of its mammalian host. We examined the effects of growth temperature on the transcriptome of the P. aeruginosa PAO1. Microarray analysis of PAO1 grown in Lysogeny broth at mid-exponential phase at 22°C and 37°C revealed that temperature changes are responsible for the differential transcriptional regulation of 6.4% of the genome. Major alterations were observed in bacterial metabolism, replication, and nutrient acquisition. Quorum-sensing and exoproteins secreted by type I, II, and III secretion systems, involved in the adaptation of P. aeruginosa to the mammalian host during infection, were up-regulated at 37°C compared to 22°C. Genes encoding arginine degradation enzymes were highly up-regulated at 22°C, together with the genes involved in the synthesis of pyoverdine. However, genes involved in pyochelin biosynthesis were up-regulated at 37°C. We observed that the changes in expression of P. aeruginosa siderophores correlated to an overall increase in Fe²⁺ extracellular concentration at 37°C and a peak in Fe³⁺ extracellular concentration at 22°C. This suggests a distinct change in iron acquisition strategies when the bacterium switches from the external environment to the host. Our work identifies global changes in bacterial metabolism and nutrient acquisition induced by growth at different temperatures. Overall, this study identifies factors that are regulated in genome-wide adaptation processes and discusses how this life-threatening pathogen responds to temperature.
format	article
author	Mariette Barbier F Heath Damron Piotr Bielecki María Suárez-Diez Jacek Puchałka Sebastian Albertí Vitor Martins Dos Santos Joanna B Goldberg
author_facet	Mariette Barbier F Heath Damron Piotr Bielecki María Suárez-Diez Jacek Puchałka Sebastian Albertí Vitor Martins Dos Santos Joanna B Goldberg
author_sort	Mariette Barbier
title	From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.
title_short	From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.
title_full	From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.
title_fullStr	From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.
title_full_unstemmed	From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.
title_sort	from the environment to the host: re-wiring of the transcriptome of pseudomonas aeruginosa from 22°c to 37°c.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2049a7568cc14ca88a50993dc5a3d56b
work_keys_str_mv	AT mariettebarbier fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT fheathdamron fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT piotrbielecki fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT mariasuarezdiez fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT jacekpuchałka fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT sebastianalberti fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT vitormartinsdossantos fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c AT joannabgoldberg fromtheenvironmenttothehostrewiringofthetranscriptomeofpseudomonasaeruginosafrom22cto37c
_version_	1718421671037108224

From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22°C to 37°C.

Ejemplares similares