Increased astrocyte representation in the hippocampus of 5xFAD mice

Introduction: Alzheimer's disease is the most common neurodegenerative disorder, characterized by the formation of amyloid plaques and the neurofibrillary tangles in the brain of an ill person, leading to neuronal damage and loss. Activation of astrocytes and astrogliosis occurs along with this...

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Autores principales: Jovanović Violeta, Despotović Jelica, Balo Mario, Zaletel Ivan, Despotović Sanja, Puškaš Nela
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Publicado: University of Belgrade, Medical Faculty 2021
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Acceso en línea:https://doaj.org/article/204b934ef4484d9484fb8dbe5982749b
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spelling oai:doaj.org-article:204b934ef4484d9484fb8dbe5982749b2021-12-05T21:26:44ZIncreased astrocyte representation in the hippocampus of 5xFAD mice0369-15272466-552510.5937/mp72-32451https://doaj.org/article/204b934ef4484d9484fb8dbe5982749b2021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2021/0369-15272102005J.pdfhttps://doaj.org/toc/0369-1527https://doaj.org/toc/2466-5525Introduction: Alzheimer's disease is the most common neurodegenerative disorder, characterized by the formation of amyloid plaques and the neurofibrillary tangles in the brain of an ill person, leading to neuronal damage and loss. Activation of astrocytes and astrogliosis occurs along with this process. Due to ethical limitations in working with human tissue, numerous transgenic animal models have been developed to study the pathogenesis of these processes. Early Ab deposition is observed in the cortex and the hippocampus. Aim: This study aimed to determine the difference in the presence of GFAP positive cells in the hippocampus between transgenic 5xFAD mice aged 36 weeks and their corresponding controls. Material and Methods: The 5xFAD mice model of Alzheimer's disease was used, characterized by early formation of amyloid plaques but without the presence of neurofibrillar tangles. Transgenic and control animals were sacrificed at 36 weeks of age. The visualization of GFAP-positive cells in the hippocampus of their brains was done by using immunohistochemistry and antibody for glial fibrillary acidic protein - GFAP, the major marker of astrocytes. Quantification of immuno-reactivity was done by using the Icy software system. Results: There was a statistically significant difference in the expression of GFAP in the dentate gyrus and the granular zone of the hippocampus between the transgenic and control group at 36 weeks of age, while the significant change in the CA1-3 regions was not observed between investigated groups. Conclusion: Obtained results confirm the involvement of astrogliosis in the pathophysiology of Alzheimer's disease and indicate an earlier occurrence of astrogliosis in the dentate gyrus and granular zone, in relation to other regions of the hippocampus, in the 36-week-old 5xFAD mice.Jovanović VioletaDespotović JelicaBalo MarioZaletel IvanDespotović SanjaPuškaš NelaUniversity of Belgrade, Medical Facultyarticlealzheimer's disease5xfadhippocampusastrocytesgfapMedicineRENSRMedicinski Podmladak, Vol 72, Iss 2, Pp 5-10 (2021)
institution DOAJ
collection DOAJ
language EN
SR
topic alzheimer's disease
5xfad
hippocampus
astrocytes
gfap
Medicine
R
spellingShingle alzheimer's disease
5xfad
hippocampus
astrocytes
gfap
Medicine
R
Jovanović Violeta
Despotović Jelica
Balo Mario
Zaletel Ivan
Despotović Sanja
Puškaš Nela
Increased astrocyte representation in the hippocampus of 5xFAD mice
description Introduction: Alzheimer's disease is the most common neurodegenerative disorder, characterized by the formation of amyloid plaques and the neurofibrillary tangles in the brain of an ill person, leading to neuronal damage and loss. Activation of astrocytes and astrogliosis occurs along with this process. Due to ethical limitations in working with human tissue, numerous transgenic animal models have been developed to study the pathogenesis of these processes. Early Ab deposition is observed in the cortex and the hippocampus. Aim: This study aimed to determine the difference in the presence of GFAP positive cells in the hippocampus between transgenic 5xFAD mice aged 36 weeks and their corresponding controls. Material and Methods: The 5xFAD mice model of Alzheimer's disease was used, characterized by early formation of amyloid plaques but without the presence of neurofibrillar tangles. Transgenic and control animals were sacrificed at 36 weeks of age. The visualization of GFAP-positive cells in the hippocampus of their brains was done by using immunohistochemistry and antibody for glial fibrillary acidic protein - GFAP, the major marker of astrocytes. Quantification of immuno-reactivity was done by using the Icy software system. Results: There was a statistically significant difference in the expression of GFAP in the dentate gyrus and the granular zone of the hippocampus between the transgenic and control group at 36 weeks of age, while the significant change in the CA1-3 regions was not observed between investigated groups. Conclusion: Obtained results confirm the involvement of astrogliosis in the pathophysiology of Alzheimer's disease and indicate an earlier occurrence of astrogliosis in the dentate gyrus and granular zone, in relation to other regions of the hippocampus, in the 36-week-old 5xFAD mice.
format article
author Jovanović Violeta
Despotović Jelica
Balo Mario
Zaletel Ivan
Despotović Sanja
Puškaš Nela
author_facet Jovanović Violeta
Despotović Jelica
Balo Mario
Zaletel Ivan
Despotović Sanja
Puškaš Nela
author_sort Jovanović Violeta
title Increased astrocyte representation in the hippocampus of 5xFAD mice
title_short Increased astrocyte representation in the hippocampus of 5xFAD mice
title_full Increased astrocyte representation in the hippocampus of 5xFAD mice
title_fullStr Increased astrocyte representation in the hippocampus of 5xFAD mice
title_full_unstemmed Increased astrocyte representation in the hippocampus of 5xFAD mice
title_sort increased astrocyte representation in the hippocampus of 5xfad mice
publisher University of Belgrade, Medical Faculty
publishDate 2021
url https://doaj.org/article/204b934ef4484d9484fb8dbe5982749b
work_keys_str_mv AT jovanovicvioleta increasedastrocyterepresentationinthehippocampusof5xfadmice
AT despotovicjelica increasedastrocyterepresentationinthehippocampusof5xfadmice
AT balomario increasedastrocyterepresentationinthehippocampusof5xfadmice
AT zaletelivan increasedastrocyterepresentationinthehippocampusof5xfadmice
AT despotovicsanja increasedastrocyterepresentationinthehippocampusof5xfadmice
AT puskasnela increasedastrocyterepresentationinthehippocampusof5xfadmice
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