Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis

Bovine mastitis is a costly disease in the dairy farms globally. The control of such disease is generally based on the prevention by the strict hygienic measures during milking. Other approaches include vaccination and the application of antibiotics. Regardless to these procedures, mastitis is not e...

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Autores principales:	Kamel, H. M., Armanious, W., Morgan, S., Ebtsam E.Z. Kotb
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	bovine mastitis lactobacillus maldi-tof ms probiotics s. aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://doaj.org/article/204f8f106fab4bb79dda7f8d071b302d
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spelling	oai:doaj.org-article:204f8f106fab4bb79dda7f8d071b302d2021-12-02T16:23:42ZExploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis 10.21608/javs.2021.79310.10831687-40722090-3308https://doaj.org/article/204f8f106fab4bb79dda7f8d071b302d2021-07-01T00:00:00Zhttps://javs.journals.ekb.eg/article_183871.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Bovine mastitis is a costly disease in the dairy farms globally. The control of such disease is generally based on the prevention by the strict hygienic measures during milking. Other approaches include vaccination and the application of antibiotics. Regardless to these procedures, mastitis is not entirely under the control, thus increasing the need for alternative tactics. This study was conducted to isolate and identify lactic acid bacteria (LAB) from fresh cow’s milk which possess antibacterial activity that could be used for mastitis control. 146 isolates were recognized as (LAB) from105 milk tanks samples after being cultured anaerobically on de Man, Rogosa and Sharpe (MRS) agar plates for 48 hours at 37 oC and identified by general bacteriological investigation. Afterwards, 24 isolates were identified to belong to genus Lactobacillus using polymerase chain reaction (PCR), and for species level recognition MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) was used resulted in : L. fermentum (5), L. brevis (3), L. plantarum (4), L. paracasei (2), L. rhamnosus (3), L. pentosus (2), L. casei (3), L. raffinolactis (1) and L. mesenteroids (1). The antimicrobial activity of these strains against one of the major mastitis pathogens, S. aureus, was detected by the agar well diffusion assay and the modified double layer method , where L. casei, L. fermentum and L. plantarum possess the most inhibiting effect besides they have no hemolytic nor gelatin liquefaction activity when their safety profiles were evaluated. The result of the antibiotic susceptibility test revealed that these isolates were resistant to vancomycin (VA), neomycin (N) and gentamycin (CN). On the other hands, they were highly sensitive to amoxicillin clavulanic acid (AMC), levamisole (LEV), tetracycline (TE) and penicillin (P). The study suggests that L. casei, L. fermentum and L. plantarum are perfect candidates to be used as probiotics to help in preventing and controlling bovine mastitis caused by Staphylococcus aureus as they were proven to be safe and have antimicrobial activity against the organism. Kamel, H. M. Armanious, W. Morgan, S. Ebtsam E.Z. KotbEgyptian Society for Animal Managementarticlebovine mastitislactobacillusmaldi-tof msprobioticss. aureusZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 3, Pp 60-69 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine mastitis lactobacillus maldi-tof ms probiotics s. aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	bovine mastitis lactobacillus maldi-tof ms probiotics s. aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Kamel, H. M. Armanious, W. Morgan, S. Ebtsam E.Z. Kotb Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis
description	Bovine mastitis is a costly disease in the dairy farms globally. The control of such disease is generally based on the prevention by the strict hygienic measures during milking. Other approaches include vaccination and the application of antibiotics. Regardless to these procedures, mastitis is not entirely under the control, thus increasing the need for alternative tactics. This study was conducted to isolate and identify lactic acid bacteria (LAB) from fresh cow’s milk which possess antibacterial activity that could be used for mastitis control. 146 isolates were recognized as (LAB) from105 milk tanks samples after being cultured anaerobically on de Man, Rogosa and Sharpe (MRS) agar plates for 48 hours at 37 oC and identified by general bacteriological investigation. Afterwards, 24 isolates were identified to belong to genus Lactobacillus using polymerase chain reaction (PCR), and for species level recognition MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) was used resulted in : L. fermentum (5), L. brevis (3), L. plantarum (4), L. paracasei (2), L. rhamnosus (3), L. pentosus (2), L. casei (3), L. raffinolactis (1) and L. mesenteroids (1). The antimicrobial activity of these strains against one of the major mastitis pathogens, S. aureus, was detected by the agar well diffusion assay and the modified double layer method , where L. casei, L. fermentum and L. plantarum possess the most inhibiting effect besides they have no hemolytic nor gelatin liquefaction activity when their safety profiles were evaluated. The result of the antibiotic susceptibility test revealed that these isolates were resistant to vancomycin (VA), neomycin (N) and gentamycin (CN). On the other hands, they were highly sensitive to amoxicillin clavulanic acid (AMC), levamisole (LEV), tetracycline (TE) and penicillin (P). The study suggests that L. casei, L. fermentum and L. plantarum are perfect candidates to be used as probiotics to help in preventing and controlling bovine mastitis caused by Staphylococcus aureus as they were proven to be safe and have antimicrobial activity against the organism.
format	article
author	Kamel, H. M. Armanious, W. Morgan, S. Ebtsam E.Z. Kotb
author_facet	Kamel, H. M. Armanious, W. Morgan, S. Ebtsam E.Z. Kotb
author_sort	Kamel, H. M.
title	Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis
title_short	Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis
title_full	Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis
title_fullStr	Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis
title_full_unstemmed	Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis
title_sort	exploring antimicrobial activity of lactobacillus spp. (probiotics) isolated from raw cow’s milk against staphylococcus aureus causing bovine mastitis
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://doaj.org/article/204f8f106fab4bb79dda7f8d071b302d
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Exploring antimicrobial activity of Lactobacillus spp. (probiotics) isolated from raw cow’s milk against Staphylococcus aureus causing bovine mastitis

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