Molecular, biochemical and genetic characteristics of BSE in Canada.

The epidemiology and possibly the etiology of bovine spongiform encephalopathy (BSE) have recently been recognized to be heterogeneous. In particular, three types [classical (C) and two atypical (H, L)] have been identified, largely on the basis of characteristics of the proteinase K (PK)-resistant...

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Autores principales:	Sandor Dudas, Jianmin Yang, Catherine Graham, Markus Czub, Tim A McAllister, Michael B Coulthart, Stefanie Czub
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/205181c7c8cc4ddca46c924d47c456be
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spelling	oai:doaj.org-article:205181c7c8cc4ddca46c924d47c456be2021-12-02T20:21:39ZMolecular, biochemical and genetic characteristics of BSE in Canada.1932-620310.1371/journal.pone.0010638https://doaj.org/article/205181c7c8cc4ddca46c924d47c456be2010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20498835/?tool=EBIhttps://doaj.org/toc/1932-6203The epidemiology and possibly the etiology of bovine spongiform encephalopathy (BSE) have recently been recognized to be heterogeneous. In particular, three types [classical (C) and two atypical (H, L)] have been identified, largely on the basis of characteristics of the proteinase K (PK)-resistant core of the misfolded prion protein associated with the disease (PrP(res)). The present study was conducted to characterize the 17 Canadian BSE cases which occurred prior to November 2009 based on the molecular and biochemical properties of their PrP(res), including immunoreactivity, molecular weight, glycoform profile and relative PK sensitivity. Two cases exhibited molecular weight and glycoform profiles similar to those of previously reported atypical cases, one corresponding to H-type BSE (case 6) and the other to L-type BSE (case 11). All other cases were classified as C-type. PK digestion under mild and stringent conditions revealed a reduced protease resistance in both of these cases compared to the C-type cases. With Western immunoblotting, N-terminal-specific antibodies bound to PrP(res) from case 6 but not to that from case 11 or C-type cases. C-terminal-specific antibodies revealed a shift in the glycoform profile and detected a fourth protein fragment in case 6, indicative of two PrP(res) subpopulations in H-type BSE. No mutations suggesting a genetic etiology were found in any of the 17 animals by sequencing the full PrP-coding sequence in exon 3 of the PRNP gene. Thus, each of the three known BSE types have been confirmed in Canadian cattle and show molecular characteristics highly similar to those of classical and atypical BSE cases described from Europe, Japan and the USA. The occurrence of atypical cases of BSE in countries such as Canada with low BSE prevalence and transmission risk argues for the occurrence of sporadic forms of BSE worldwide.Sandor DudasJianmin YangCatherine GrahamMarkus CzubTim A McAllisterMichael B CoulthartStefanie CzubPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10638 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandor Dudas Jianmin Yang Catherine Graham Markus Czub Tim A McAllister Michael B Coulthart Stefanie Czub Molecular, biochemical and genetic characteristics of BSE in Canada.
description	The epidemiology and possibly the etiology of bovine spongiform encephalopathy (BSE) have recently been recognized to be heterogeneous. In particular, three types [classical (C) and two atypical (H, L)] have been identified, largely on the basis of characteristics of the proteinase K (PK)-resistant core of the misfolded prion protein associated with the disease (PrP(res)). The present study was conducted to characterize the 17 Canadian BSE cases which occurred prior to November 2009 based on the molecular and biochemical properties of their PrP(res), including immunoreactivity, molecular weight, glycoform profile and relative PK sensitivity. Two cases exhibited molecular weight and glycoform profiles similar to those of previously reported atypical cases, one corresponding to H-type BSE (case 6) and the other to L-type BSE (case 11). All other cases were classified as C-type. PK digestion under mild and stringent conditions revealed a reduced protease resistance in both of these cases compared to the C-type cases. With Western immunoblotting, N-terminal-specific antibodies bound to PrP(res) from case 6 but not to that from case 11 or C-type cases. C-terminal-specific antibodies revealed a shift in the glycoform profile and detected a fourth protein fragment in case 6, indicative of two PrP(res) subpopulations in H-type BSE. No mutations suggesting a genetic etiology were found in any of the 17 animals by sequencing the full PrP-coding sequence in exon 3 of the PRNP gene. Thus, each of the three known BSE types have been confirmed in Canadian cattle and show molecular characteristics highly similar to those of classical and atypical BSE cases described from Europe, Japan and the USA. The occurrence of atypical cases of BSE in countries such as Canada with low BSE prevalence and transmission risk argues for the occurrence of sporadic forms of BSE worldwide.
format	article
author	Sandor Dudas Jianmin Yang Catherine Graham Markus Czub Tim A McAllister Michael B Coulthart Stefanie Czub
author_facet	Sandor Dudas Jianmin Yang Catherine Graham Markus Czub Tim A McAllister Michael B Coulthart Stefanie Czub
author_sort	Sandor Dudas
title	Molecular, biochemical and genetic characteristics of BSE in Canada.
title_short	Molecular, biochemical and genetic characteristics of BSE in Canada.
title_full	Molecular, biochemical and genetic characteristics of BSE in Canada.
title_fullStr	Molecular, biochemical and genetic characteristics of BSE in Canada.
title_full_unstemmed	Molecular, biochemical and genetic characteristics of BSE in Canada.
title_sort	molecular, biochemical and genetic characteristics of bse in canada.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/205181c7c8cc4ddca46c924d47c456be
work_keys_str_mv	AT sandordudas molecularbiochemicalandgeneticcharacteristicsofbseincanada AT jianminyang molecularbiochemicalandgeneticcharacteristicsofbseincanada AT catherinegraham molecularbiochemicalandgeneticcharacteristicsofbseincanada AT markusczub molecularbiochemicalandgeneticcharacteristicsofbseincanada AT timamcallister molecularbiochemicalandgeneticcharacteristicsofbseincanada AT michaelbcoulthart molecularbiochemicalandgeneticcharacteristicsofbseincanada AT stefanieczub molecularbiochemicalandgeneticcharacteristicsofbseincanada
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Molecular, biochemical and genetic characteristics of BSE in Canada.

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