CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p

Abstract The long-noncoding RNA colorectal neoplasia differentially expressed (CRNDE) gene has been considered to be crucial in tumor malignancy. Although CRNDE is highly expressed in acute myeloid leukemia (AML), its mechanism of action remains unknown. In this study, GEPIA and qRT-PCR were perform...

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Autores principales:	Chen Liu, Liang Zhong, Chenlan Shen, Xuan Chu, Xu Luo, Lihua Yu, Jiao Ye, Ling Xiong, Wenran Dan, Jian Li, Beizhong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2062a5fd23a944c59984b7357264311e
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spelling	oai:doaj.org-article:2062a5fd23a944c59984b7357264311e2021-12-02T15:10:50ZCRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p10.1038/s41598-021-96156-32045-2322https://doaj.org/article/2062a5fd23a944c59984b7357264311e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96156-3https://doaj.org/toc/2045-2322Abstract The long-noncoding RNA colorectal neoplasia differentially expressed (CRNDE) gene has been considered to be crucial in tumor malignancy. Although CRNDE is highly expressed in acute myeloid leukemia (AML), its mechanism of action remains unknown. In this study, GEPIA and qRT-PCR were performed to confirm the expression of CRNDE in AML samples and cell lines, respectively. CRNDE shRNA vectors were transfected to explore the biological functions of CRNDE. The cell proliferation was assessed by the CCK8 assay, while apoptosis and cell cycle distribution were measured by flow cytometry and Western blotting. The results showed that CRNDE was overexpressed in both AML samples and cell lines. CRNDE silencing inhibited proliferation and increased apoptotic rate and cell cycle arrest of KG-1a cells. The luciferase reporter assay coupled with RIP assay revealed that CRNDE act as a ceRNA. Rescue assays demonstrated that the effects of CRNDE silencing could be reversed by miR-136-5p inhibitors. In conclusion, our results expound that the CRNDE/miR-136-5p/MCM5 axis modulates cell progression and provide a new regulatory network of CRNDE in KG-1a cells.Chen LiuLiang ZhongChenlan ShenXuan ChuXu LuoLihua YuJiao YeLing XiongWenran DanJian LiBeizhong LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen Liu Liang Zhong Chenlan Shen Xuan Chu Xu Luo Lihua Yu Jiao Ye Ling Xiong Wenran Dan Jian Li Beizhong Liu CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p
description	Abstract The long-noncoding RNA colorectal neoplasia differentially expressed (CRNDE) gene has been considered to be crucial in tumor malignancy. Although CRNDE is highly expressed in acute myeloid leukemia (AML), its mechanism of action remains unknown. In this study, GEPIA and qRT-PCR were performed to confirm the expression of CRNDE in AML samples and cell lines, respectively. CRNDE shRNA vectors were transfected to explore the biological functions of CRNDE. The cell proliferation was assessed by the CCK8 assay, while apoptosis and cell cycle distribution were measured by flow cytometry and Western blotting. The results showed that CRNDE was overexpressed in both AML samples and cell lines. CRNDE silencing inhibited proliferation and increased apoptotic rate and cell cycle arrest of KG-1a cells. The luciferase reporter assay coupled with RIP assay revealed that CRNDE act as a ceRNA. Rescue assays demonstrated that the effects of CRNDE silencing could be reversed by miR-136-5p inhibitors. In conclusion, our results expound that the CRNDE/miR-136-5p/MCM5 axis modulates cell progression and provide a new regulatory network of CRNDE in KG-1a cells.
format	article
author	Chen Liu Liang Zhong Chenlan Shen Xuan Chu Xu Luo Lihua Yu Jiao Ye Ling Xiong Wenran Dan Jian Li Beizhong Liu
author_facet	Chen Liu Liang Zhong Chenlan Shen Xuan Chu Xu Luo Lihua Yu Jiao Ye Ling Xiong Wenran Dan Jian Li Beizhong Liu
author_sort	Chen Liu
title	CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p
title_short	CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p
title_full	CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p
title_fullStr	CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p
title_full_unstemmed	CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p
title_sort	crnde enhances the expression of mcm5 and proliferation in acute myeloid leukemia kg-1a cells by sponging mir-136-5p
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2062a5fd23a944c59984b7357264311e
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CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p

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