Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.

<h4>Background</h4>Alterations in gene expression in the suicide brain have been reported and for several genes DNA methylation as an epigenetic regulator is thought to play a role. rRNA genes, that encode ribosomal RNA, are the backbone of the protein synthesis machinery and levels of r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Patrick O McGowan, Aya Sasaki, Tony C T Huang, Alexander Unterberger, Matthew Suderman, Carl Ernst, Michael J Meaney, Gustavo Turecki, Moshe Szyf
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20644e04490b4354bc9a449488f38d1a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:20644e04490b4354bc9a449488f38d1a
record_format	dspace
spelling	oai:doaj.org-article:20644e04490b4354bc9a449488f38d1a2021-11-25T06:12:36ZPromoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.1932-620310.1371/journal.pone.0002085https://doaj.org/article/20644e04490b4354bc9a449488f38d1a2008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18461137/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Alterations in gene expression in the suicide brain have been reported and for several genes DNA methylation as an epigenetic regulator is thought to play a role. rRNA genes, that encode ribosomal RNA, are the backbone of the protein synthesis machinery and levels of rRNA gene promoter methylation determine rRNA transcription.<h4>Methodology/principal findings</h4>We test here by sodium bisulfite mapping of the rRNA promoter and quantitative real-time PCR of rRNA expression the hypothesis that epigenetic differences in critical loci in the brain are involved in the pathophysiology of suicide. Suicide subjects in this study were selected for a history of early childhood neglect/abuse, which is associated with decreased hippocampal volume and cognitive impairments. rRNA was significantly hypermethylated throughout the promoter and 5' regulatory region in the brain of suicide subjects, consistent with reduced rRNA expression in the hippocampus. This difference in rRNA methylation was not evident in the cerebellum and occurred in the absence of genome-wide changes in methylation, as assessed by nearest neighbor.<h4>Conclusions/significance</h4>This is the first study to show aberrant regulation of the protein synthesis machinery in the suicide brain. The data implicate the epigenetic modulation of rRNA in the pathophysiology of suicide.Patrick O McGowanAya SasakiTony C T HuangAlexander UnterbergerMatthew SudermanCarl ErnstMichael J MeaneyGustavo TureckiMoshe SzyfPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 5, p e2085 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrick O McGowan Aya Sasaki Tony C T Huang Alexander Unterberger Matthew Suderman Carl Ernst Michael J Meaney Gustavo Turecki Moshe Szyf Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.
description	<h4>Background</h4>Alterations in gene expression in the suicide brain have been reported and for several genes DNA methylation as an epigenetic regulator is thought to play a role. rRNA genes, that encode ribosomal RNA, are the backbone of the protein synthesis machinery and levels of rRNA gene promoter methylation determine rRNA transcription.<h4>Methodology/principal findings</h4>We test here by sodium bisulfite mapping of the rRNA promoter and quantitative real-time PCR of rRNA expression the hypothesis that epigenetic differences in critical loci in the brain are involved in the pathophysiology of suicide. Suicide subjects in this study were selected for a history of early childhood neglect/abuse, which is associated with decreased hippocampal volume and cognitive impairments. rRNA was significantly hypermethylated throughout the promoter and 5' regulatory region in the brain of suicide subjects, consistent with reduced rRNA expression in the hippocampus. This difference in rRNA methylation was not evident in the cerebellum and occurred in the absence of genome-wide changes in methylation, as assessed by nearest neighbor.<h4>Conclusions/significance</h4>This is the first study to show aberrant regulation of the protein synthesis machinery in the suicide brain. The data implicate the epigenetic modulation of rRNA in the pathophysiology of suicide.
format	article
author	Patrick O McGowan Aya Sasaki Tony C T Huang Alexander Unterberger Matthew Suderman Carl Ernst Michael J Meaney Gustavo Turecki Moshe Szyf
author_facet	Patrick O McGowan Aya Sasaki Tony C T Huang Alexander Unterberger Matthew Suderman Carl Ernst Michael J Meaney Gustavo Turecki Moshe Szyf
author_sort	Patrick O McGowan
title	Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.
title_short	Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.
title_full	Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.
title_fullStr	Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.
title_full_unstemmed	Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.
title_sort	promoter-wide hypermethylation of the ribosomal rna gene promoter in the suicide brain.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/20644e04490b4354bc9a449488f38d1a
work_keys_str_mv	AT patrickomcgowan promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT ayasasaki promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT tonycthuang promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT alexanderunterberger promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT matthewsuderman promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT carlernst promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT michaeljmeaney promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT gustavoturecki promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain AT mosheszyf promoterwidehypermethylationoftheribosomalrnagenepromoterinthesuicidebrain
_version_	1718414029882392576

Promoter-wide hypermethylation of the ribosomal RNA gene promoter in the suicide brain.

Ejemplares similares