Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells

Abstract Exposure to cigarette smoke (CS) results in injury to the epithelial cells of the human respiratory tract and has been implicated as a causative factor in the development of chronic obstructive pulmonary disease and lung cancers. The application of omics-scale methodologies has improved the...

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Autores principales:	Hongyu Chen, Xi Chen, Yifei Shen, Xinxin Yin, Fangjie Liu, Lu Liu, Jie Yao, Qinjie Chu, Yaqin Wang, Hongyan Qi, Michael P. Timko, Weijia Fang, Longjiang Fan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2064a4ab8129436fa97ae382d23c3197
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spelling	oai:doaj.org-article:2064a4ab8129436fa97ae382d23c31972021-12-02T16:46:35ZSignaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells10.1038/s41598-021-95938-z2045-2322https://doaj.org/article/2064a4ab8129436fa97ae382d23c31972021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95938-zhttps://doaj.org/toc/2045-2322Abstract Exposure to cigarette smoke (CS) results in injury to the epithelial cells of the human respiratory tract and has been implicated as a causative factor in the development of chronic obstructive pulmonary disease and lung cancers. The application of omics-scale methodologies has improved the capacity to understand cellular signaling processes underlying response to CS exposure. We report here the development of an algorithm based on quantitative assessment of transcriptomic profiles and signaling pathway perturbation analysis (SPPA) of human bronchial epithelial cells (HBEC) exposed to the toxic components present in CS. HBEC were exposed to CS of different compositions and for different durations using an ISO3308 smoking regime and the impact of exposure was monitored in 2263 signaling pathways in the cell to generate a total effect score that reflects the quantitative degree of impact of external stimuli on the cells. These findings support the conclusion that the SPPA algorithm provides an objective, systematic, sensitive means to evaluate the biological impact of exposures to CS of different compositions making a powerful comparative tool for commercial product evaluation and potentially for other known or potentially toxic environmental smoke substances.Hongyu ChenXi ChenYifei ShenXinxin YinFangjie LiuLu LiuJie YaoQinjie ChuYaqin WangHongyan QiMichael P. TimkoWeijia FangLongjiang FanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongyu Chen Xi Chen Yifei Shen Xinxin Yin Fangjie Liu Lu Liu Jie Yao Qinjie Chu Yaqin Wang Hongyan Qi Michael P. Timko Weijia Fang Longjiang Fan Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
description	Abstract Exposure to cigarette smoke (CS) results in injury to the epithelial cells of the human respiratory tract and has been implicated as a causative factor in the development of chronic obstructive pulmonary disease and lung cancers. The application of omics-scale methodologies has improved the capacity to understand cellular signaling processes underlying response to CS exposure. We report here the development of an algorithm based on quantitative assessment of transcriptomic profiles and signaling pathway perturbation analysis (SPPA) of human bronchial epithelial cells (HBEC) exposed to the toxic components present in CS. HBEC were exposed to CS of different compositions and for different durations using an ISO3308 smoking regime and the impact of exposure was monitored in 2263 signaling pathways in the cell to generate a total effect score that reflects the quantitative degree of impact of external stimuli on the cells. These findings support the conclusion that the SPPA algorithm provides an objective, systematic, sensitive means to evaluate the biological impact of exposures to CS of different compositions making a powerful comparative tool for commercial product evaluation and potentially for other known or potentially toxic environmental smoke substances.
format	article
author	Hongyu Chen Xi Chen Yifei Shen Xinxin Yin Fangjie Liu Lu Liu Jie Yao Qinjie Chu Yaqin Wang Hongyan Qi Michael P. Timko Weijia Fang Longjiang Fan
author_facet	Hongyu Chen Xi Chen Yifei Shen Xinxin Yin Fangjie Liu Lu Liu Jie Yao Qinjie Chu Yaqin Wang Hongyan Qi Michael P. Timko Weijia Fang Longjiang Fan
author_sort	Hongyu Chen
title	Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
title_short	Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
title_full	Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
title_fullStr	Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
title_full_unstemmed	Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
title_sort	signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2064a4ab8129436fa97ae382d23c3197
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_version_	1718383381593456640

Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells

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