Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura & Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zeb...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shengchang Tao, Zhiyao Ren, Zerui Yang, Shuna Duan, Zhongxian Wan, Jiahui Huang, Chenxing Liu, Gang Wei
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Dendrobium officinale Kimura & Migo polysaccharide molecular weight colorectal cancer Astragalus membranaceus polysaccharides Lentinus edodes polysaccharides Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2069f4cd494b4f2bb1c6da02107ab661
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2069f4cd494b4f2bb1c6da02107ab661
record_format	dspace
spelling	oai:doaj.org-article:2069f4cd494b4f2bb1c6da02107ab6612021-12-03T06:57:43ZEffects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes1663-981210.3389/fphar.2021.704486https://doaj.org/article/2069f4cd494b4f2bb1c6da02107ab6612021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.704486/fullhttps://doaj.org/toc/1663-9812We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura & Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of D. officinale polysaccharides. Fractions of D. officinale polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of D. officinale polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1–treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway “apoptosis-multiple species” was the most significantly enriched. Our data indicated that 1) fractions of D. officinale polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.Shengchang TaoShengchang TaoZhiyao RenZhiyao RenZhiyao RenZerui YangShuna DuanShuna DuanZhongxian WanJiahui HuangJiahui HuangChenxing LiuChenxing LiuGang WeiFrontiers Media S.A.articleDendrobium officinale Kimura & Migopolysaccharidemolecular weightcolorectal cancerAstragalus membranaceus polysaccharidesLentinus edodes polysaccharidesTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dendrobium officinale Kimura & Migo polysaccharide molecular weight colorectal cancer Astragalus membranaceus polysaccharides Lentinus edodes polysaccharides Therapeutics. Pharmacology RM1-950
spellingShingle	Dendrobium officinale Kimura & Migo polysaccharide molecular weight colorectal cancer Astragalus membranaceus polysaccharides Lentinus edodes polysaccharides Therapeutics. Pharmacology RM1-950 Shengchang Tao Shengchang Tao Zhiyao Ren Zhiyao Ren Zhiyao Ren Zerui Yang Shuna Duan Shuna Duan Zhongxian Wan Jiahui Huang Jiahui Huang Chenxing Liu Chenxing Liu Gang Wei Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
description	We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura & Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of D. officinale polysaccharides. Fractions of D. officinale polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of D. officinale polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1–treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway “apoptosis-multiple species” was the most significantly enriched. Our data indicated that 1) fractions of D. officinale polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.
format	article
author	Shengchang Tao Shengchang Tao Zhiyao Ren Zhiyao Ren Zhiyao Ren Zerui Yang Shuna Duan Shuna Duan Zhongxian Wan Jiahui Huang Jiahui Huang Chenxing Liu Chenxing Liu Gang Wei
author_facet	Shengchang Tao Shengchang Tao Zhiyao Ren Zhiyao Ren Zhiyao Ren Zerui Yang Shuna Duan Shuna Duan Zhongxian Wan Jiahui Huang Jiahui Huang Chenxing Liu Chenxing Liu Gang Wei
author_sort	Shengchang Tao
title	Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
title_short	Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
title_full	Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
title_fullStr	Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
title_full_unstemmed	Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
title_sort	effects of different molecular weight polysaccharides from dendrobium officinale kimura & migo on human colorectal cancer and transcriptome analysis of differentially expressed genes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2069f4cd494b4f2bb1c6da02107ab661
work_keys_str_mv	AT shengchangtao effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT shengchangtao effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT zhiyaoren effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT zhiyaoren effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT zhiyaoren effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT zeruiyang effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT shunaduan effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT shunaduan effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT zhongxianwan effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT jiahuihuang effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT jiahuihuang effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT chenxingliu effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT chenxingliu effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes AT gangwei effectsofdifferentmolecularweightpolysaccharidesfromdendrobiumofficinalekimuramigoonhumancolorectalcancerandtranscriptomeanalysisofdifferentiallyexpressedgenes
_version_	1718373859060613120

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

Ejemplares similares