Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest

Abstract STAG3 is essential for male meiosis and testis of male Stag3 −/− mice shows the histopathological type of germ cell maturation arrest (MA). Whether variants of the STAG3 gene exist in Chinese idiopathic non-obstructive azoospermia (NOA) patients needs to be determined. We recruited 58 Chine...

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Autores principales:	Wen Liu, Xuan Gao, Haobo Zhang, Ran Liu, Yongzhi Cao, Ruimei Yu, Ge Fang, Jinlong Ma, Shidou Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/206d7307483f4e648834a34754f8e5f1
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spelling	oai:doaj.org-article:206d7307483f4e648834a34754f8e5f12021-12-02T16:50:31ZAnalysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest10.1038/s41598-021-89559-92045-2322https://doaj.org/article/206d7307483f4e648834a34754f8e5f12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89559-9https://doaj.org/toc/2045-2322Abstract STAG3 is essential for male meiosis and testis of male Stag3 −/− mice shows the histopathological type of germ cell maturation arrest (MA). Whether variants of the STAG3 gene exist in Chinese idiopathic non-obstructive azoospermia (NOA) patients needs to be determined. We recruited 58 Chinese NOA men with MA who underwent testis biopsy and 192 fertile men as the control group. The 34 exons of the STAG3 gene were amplified using polymerase chain reaction (PCR) and sequenced. We identified eight novel single nucleotide polymorphisms (SNPs), including two missense SNPs (c.433T > C in exon2 and c.553A > G in exon3), three synonymous SNPs (c.539G > A, c.569C > T in exon3, and c.1176C > G in exon8), and three SNPs in introns. The allele and genotype frequencies of the novel and other SNPs have no significant differences between two groups. Our results indicated that variants in the coding sequence of the STAG3 gene were uncommon in NOA patients with MA in Chinese population. Future studies in large cohorts of different ethnic populations will be needed to determine the association between the STAG3 gene and NOA.Wen LiuXuan GaoHaobo ZhangRan LiuYongzhi CaoRuimei YuGe FangJinlong MaShidou ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen Liu Xuan Gao Haobo Zhang Ran Liu Yongzhi Cao Ruimei Yu Ge Fang Jinlong Ma Shidou Zhao Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest
description	Abstract STAG3 is essential for male meiosis and testis of male Stag3 −/− mice shows the histopathological type of germ cell maturation arrest (MA). Whether variants of the STAG3 gene exist in Chinese idiopathic non-obstructive azoospermia (NOA) patients needs to be determined. We recruited 58 Chinese NOA men with MA who underwent testis biopsy and 192 fertile men as the control group. The 34 exons of the STAG3 gene were amplified using polymerase chain reaction (PCR) and sequenced. We identified eight novel single nucleotide polymorphisms (SNPs), including two missense SNPs (c.433T > C in exon2 and c.553A > G in exon3), three synonymous SNPs (c.539G > A, c.569C > T in exon3, and c.1176C > G in exon8), and three SNPs in introns. The allele and genotype frequencies of the novel and other SNPs have no significant differences between two groups. Our results indicated that variants in the coding sequence of the STAG3 gene were uncommon in NOA patients with MA in Chinese population. Future studies in large cohorts of different ethnic populations will be needed to determine the association between the STAG3 gene and NOA.
format	article
author	Wen Liu Xuan Gao Haobo Zhang Ran Liu Yongzhi Cao Ruimei Yu Ge Fang Jinlong Ma Shidou Zhao
author_facet	Wen Liu Xuan Gao Haobo Zhang Ran Liu Yongzhi Cao Ruimei Yu Ge Fang Jinlong Ma Shidou Zhao
author_sort	Wen Liu
title	Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest
title_short	Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest
title_full	Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest
title_fullStr	Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest
title_full_unstemmed	Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest
title_sort	analysis of stag3 variants in chinese non-obstructive azoospermia patients with germ cell maturation arrest
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/206d7307483f4e648834a34754f8e5f1
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Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest

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