INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS

Modulatory effects of three probiotic bacterial strains (Lactobacillus rhamnosus K32 (L), Bifidobacterium longum GT15 (B, Enterococcus faecium L-3 (E) on expression level and contents of key cytokines were studied using PCR techniques with reverse transcription, and enzyme-linked immunosorbent assay...

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Autores principales:	O. V. Averina, E. I. Ermolenko, A. Yu. Ratushniy, E. A. Tarasova, Yu. Yu. Borschev, G. F. Leontieva, T. A. Kramskaya, M. P. Kotyleva, V. N. Danilenko, A. N. Suvorov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	lactobacilli bifidobacteria enterococci dysbiosis cytokines Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/206ee7df86b245d8a113f1d53ceba008
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spelling	oai:doaj.org-article:206ee7df86b245d8a113f1d53ceba0082021-11-18T08:03:45ZINFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS1563-06252313-741X10.15789/1563-0625-2015-5-443-454https://doaj.org/article/206ee7df86b245d8a113f1d53ceba0082015-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/932https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XModulatory effects of three probiotic bacterial strains (Lactobacillus rhamnosus K32 (L), Bifidobacterium longum GT15 (B, Enterococcus faecium L-3 (E) on expression level and contents of key cytokines were studied using PCR techniques with reverse transcription, and enzyme-linked immunosorbent assay. Both cell cultures and an experimental model of intestinal dysbiosis were used in this study.The genes encoding bacteriocins, surface membrane component, pili and exopolysaccharides involved in host immune system modulation were previously identified in the B and Ebacterial strains.Investigation of probiotic strains and effects of their supernatants expression of cytokines in cell cultures of promonocyte origin (HTP-1) showed increased expression of TNFα, due to E and L supernatants. Moreover, the Bl culture induced IL-8 and IL-10 expression.In a model of Wistar rats with ampicillinand metronidazole-induced intestinal dysbiosis corrected with probiotics we have shown that the dysbiosis was accompanied by sufficient alterations in microbiota composition (Klebsiella spp. overgrowth and low contents of Faecalobacterium prausnitzii) that were observed only in the animals untreated with probiotics (control), or after administration of L.In contrast to these results, the animals treated with E and B, the following changes were revealed: 1) low expression of proinflammatory cytokines IL-8, TNFα, MCP-1 inmesenteric lymph nodes and appropriate changes of their serum contents, 2) increased serum content of the anti-inflammatory TGFβ cytokine. Hence, the present study, having used two complementary models, has detected some individual features of immune modulation produced by the probiotictic strains of L. rhamnosus K32, B. longum GT15 и E. faecium L-3 which exert differential effects upon the intestinal microbiota.O. V. AverinaE. I. ErmolenkoA. Yu. RatushniyE. A. TarasovaYu. Yu. BorschevG. F. LeontievaT. A. KramskayaM. P. KotylevaV. N. DanilenkoA. N. SuvorovSPb RAACIarticlelactobacillibifidobacteriaenterococcidysbiosiscytokinesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 5, Pp 443-454 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	lactobacilli bifidobacteria enterococci dysbiosis cytokines Immunologic diseases. Allergy RC581-607
spellingShingle	lactobacilli bifidobacteria enterococci dysbiosis cytokines Immunologic diseases. Allergy RC581-607 O. V. Averina E. I. Ermolenko A. Yu. Ratushniy E. A. Tarasova Yu. Yu. Borschev G. F. Leontieva T. A. Kramskaya M. P. Kotyleva V. N. Danilenko A. N. Suvorov INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
description	Modulatory effects of three probiotic bacterial strains (Lactobacillus rhamnosus K32 (L), Bifidobacterium longum GT15 (B, Enterococcus faecium L-3 (E) on expression level and contents of key cytokines were studied using PCR techniques with reverse transcription, and enzyme-linked immunosorbent assay. Both cell cultures and an experimental model of intestinal dysbiosis were used in this study.The genes encoding bacteriocins, surface membrane component, pili and exopolysaccharides involved in host immune system modulation were previously identified in the B and Ebacterial strains.Investigation of probiotic strains and effects of their supernatants expression of cytokines in cell cultures of promonocyte origin (HTP-1) showed increased expression of TNFα, due to E and L supernatants. Moreover, the Bl culture induced IL-8 and IL-10 expression.In a model of Wistar rats with ampicillinand metronidazole-induced intestinal dysbiosis corrected with probiotics we have shown that the dysbiosis was accompanied by sufficient alterations in microbiota composition (Klebsiella spp. overgrowth and low contents of Faecalobacterium prausnitzii) that were observed only in the animals untreated with probiotics (control), or after administration of L.In contrast to these results, the animals treated with E and B, the following changes were revealed: 1) low expression of proinflammatory cytokines IL-8, TNFα, MCP-1 inmesenteric lymph nodes and appropriate changes of their serum contents, 2) increased serum content of the anti-inflammatory TGFβ cytokine. Hence, the present study, having used two complementary models, has detected some individual features of immune modulation produced by the probiotictic strains of L. rhamnosus K32, B. longum GT15 и E. faecium L-3 which exert differential effects upon the intestinal microbiota.
format	article
author	O. V. Averina E. I. Ermolenko A. Yu. Ratushniy E. A. Tarasova Yu. Yu. Borschev G. F. Leontieva T. A. Kramskaya M. P. Kotyleva V. N. Danilenko A. N. Suvorov
author_facet	O. V. Averina E. I. Ermolenko A. Yu. Ratushniy E. A. Tarasova Yu. Yu. Borschev G. F. Leontieva T. A. Kramskaya M. P. Kotyleva V. N. Danilenko A. N. Suvorov
author_sort	O. V. Averina
title	INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
title_short	INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
title_full	INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
title_fullStr	INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
title_full_unstemmed	INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
title_sort	influence of probiotics on cytokine production in the in vitro and in vivo systems
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/206ee7df86b245d8a113f1d53ceba008
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INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS

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