IN VITRO INHIBITION OF Bothrops asper VENOM BY ETHANOL EXTRACTS FROM Brownea ariza B. (CAESALPINIACEAE).

In Colombia, about 90% of snakebites are caused by Bothrops snakes, whose venoms cause in vivo, local and systemic disturbances, such as edema, myonecrosis, blood-clotting disorders and hemorrhage. Antivenom is the only effective treatment to manage these poisonings, but its limited action at local...

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Autores principales:	Viviana L. MACK-WEN, Leidy B. RICO G., Juan C. ALARCON P., Jaime A. PEREAÑEZ J.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2011
Materias:	Bothrops asper Brownea ariza viper venom proteolytic activity phospholipases A2. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/2072c13f01464dd2ac523d305a7cc876
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spelling	oai:doaj.org-article:2072c13f01464dd2ac523d305a7cc8762021-11-19T04:14:07Z<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).0121-40042145-2660https://doaj.org/article/2072c13f01464dd2ac523d305a7cc8762011-05-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/8776https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660In Colombia, about 90% of snakebites are caused by Bothrops snakes, whose venoms cause in vivo, local and systemic disturbances, such as edema, myonecrosis, blood-clotting disorders and hemorrhage. Antivenom is the only effective treatment to manage these poisonings, but its limited action at local level and little availability in geographically isolated areas makes necessary the validation and search for therapeutic alternatives acting as an immediate resource to reduce the percent of sequels caused in these snakebites. Recent studies have reported the inhibitory action of the Brownea rosademonte (Caesalpiniaceae) extract against some local effects produced by these snakes. Therefore, in this in vitro research the inhibitory ability of the ethanol extract of leaves and bark from Brownea ariza was evaluated on proteolytic, phospholipase A2 and coagulant activities of B. asper venom, which is related with anticoagulation observed in vivo. The results showed that bark extracts inhibit phospholipase A2 activity (93,2 ± 0,4%), extend the clotting time of plasma (more than 10 minutes) and inhibit the proteolytic activity, although the latter showed lesser effects than those observed with leaves extract (77 ± 0,6% and 93,8 ± 0,6%, respectively).Viviana L. MACK-WENLeidy B. RICO G.Juan C. ALARCON P.Jaime A. PEREAÑEZ J.Universidad de AntioquiaarticleBothrops asperBrownea arizaviper venomproteolytic activityphospholipases A2.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 18, Iss 1 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bothrops asper Brownea ariza viper venom proteolytic activity phospholipases A2. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Bothrops asper Brownea ariza viper venom proteolytic activity phospholipases A2. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Viviana L. MACK-WEN Leidy B. RICO G. Juan C. ALARCON P. Jaime A. PEREAÑEZ J. <I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).
description	In Colombia, about 90% of snakebites are caused by Bothrops snakes, whose venoms cause in vivo, local and systemic disturbances, such as edema, myonecrosis, blood-clotting disorders and hemorrhage. Antivenom is the only effective treatment to manage these poisonings, but its limited action at local level and little availability in geographically isolated areas makes necessary the validation and search for therapeutic alternatives acting as an immediate resource to reduce the percent of sequels caused in these snakebites. Recent studies have reported the inhibitory action of the Brownea rosademonte (Caesalpiniaceae) extract against some local effects produced by these snakes. Therefore, in this in vitro research the inhibitory ability of the ethanol extract of leaves and bark from Brownea ariza was evaluated on proteolytic, phospholipase A2 and coagulant activities of B. asper venom, which is related with anticoagulation observed in vivo. The results showed that bark extracts inhibit phospholipase A2 activity (93,2 ± 0,4%), extend the clotting time of plasma (more than 10 minutes) and inhibit the proteolytic activity, although the latter showed lesser effects than those observed with leaves extract (77 ± 0,6% and 93,8 ± 0,6%, respectively).
format	article
author	Viviana L. MACK-WEN Leidy B. RICO G. Juan C. ALARCON P. Jaime A. PEREAÑEZ J.
author_facet	Viviana L. MACK-WEN Leidy B. RICO G. Juan C. ALARCON P. Jaime A. PEREAÑEZ J.
author_sort	Viviana L. MACK-WEN
title	<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).
title_short	<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).
title_full	<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).
title_fullStr	<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).
title_full_unstemmed	<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).
title_sort	<i>in vitro</i> inhibition of <i>bothrops asper</i> venom by ethanol extracts from <i>brownea ariza b</i>. (caesalpiniaceae).
publisher	Universidad de Antioquia
publishDate	2011
url	https://doaj.org/article/2072c13f01464dd2ac523d305a7cc876
work_keys_str_mv	AT vivianalmackwen iinvitroiinhibitionofibothropsasperivenombyethanolextractsfromibrowneaarizabicaesalpiniaceae AT leidybricog iinvitroiinhibitionofibothropsasperivenombyethanolextractsfromibrowneaarizabicaesalpiniaceae AT juancalarconp iinvitroiinhibitionofibothropsasperivenombyethanolextractsfromibrowneaarizabicaesalpiniaceae AT jaimeapereanezj iinvitroiinhibitionofibothropsasperivenombyethanolextractsfromibrowneaarizabicaesalpiniaceae
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<I>IN VITRO</I> INHIBITION OF <I>Bothrops asper</I> VENOM BY ETHANOL EXTRACTS FROM <I>Brownea ariza B</I>. (CAESALPINIACEAE).

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