Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system

Abstract Oncolytic virotherapy has the disadvantage of being unsuitable for systemic delivery due to immune elimination. Liposomal encapsulation is well-recognized to reduce immune elimination and enhance the stability of drugs in the bloodstream. In the present study, the potential of liposome-enca...

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Autores principales:	Katsuyuki Aoyama, Shinji Kuroda, Toshiaki Morihiro, Nobuhiko Kanaya, Tetsushi Kubota, Yoshihiko Kakiuchi, Satoru Kikuchi, Masahiko Nishizaki, Shunsuke Kagawa, Hiroshi Tazawa, Toshiyoshi Fujiwara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20760a868f17433c8d8ecd81b0ee0a17
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spelling	oai:doaj.org-article:20760a868f17433c8d8ecd81b0ee0a172021-12-02T15:04:53ZLiposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system10.1038/s41598-017-14717-x2045-2322https://doaj.org/article/20760a868f17433c8d8ecd81b0ee0a172017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14717-xhttps://doaj.org/toc/2045-2322Abstract Oncolytic virotherapy has the disadvantage of being unsuitable for systemic delivery due to immune elimination. Liposomal encapsulation is well-recognized to reduce immune elimination and enhance the stability of drugs in the bloodstream. In the present study, the potential of liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus (TelomeScan) expressing GFP (Lipo-pTS) as an oncolytic adenoviral agent suitable for systemic delivery was investigated. Lipo-pTS, which has a diameter of 40–50 nm, showed potent antitumor effects on HCT116 colon carcinoma cells in vitro and in vivo. Tumor selectivity of Lipo-pTS was independent of coxsackie and adenovirus receptor (CAR). Importantly, Lipo-pTS reduced production of adenovirus-neutralizing antibodies (AdNAbs) after intravenous administration into immune-competent mice compared to TelomeScan, and even in the presence of AdNAbs, Lipo-pTS maintained strong cytotoxicity. In conclusion, Lipo-pTS has the potential to become an oncolytic adenoviral agent suitable for systemic delivery with the characteristics of CAR-independent antitumor activity and a stealth effect on the immune system.Katsuyuki AoyamaShinji KurodaToshiaki MorihiroNobuhiko KanayaTetsushi KubotaYoshihiko KakiuchiSatoru KikuchiMasahiko NishizakiShunsuke KagawaHiroshi TazawaToshiyoshi FujiwaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katsuyuki Aoyama Shinji Kuroda Toshiaki Morihiro Nobuhiko Kanaya Tetsushi Kubota Yoshihiko Kakiuchi Satoru Kikuchi Masahiko Nishizaki Shunsuke Kagawa Hiroshi Tazawa Toshiyoshi Fujiwara Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
description	Abstract Oncolytic virotherapy has the disadvantage of being unsuitable for systemic delivery due to immune elimination. Liposomal encapsulation is well-recognized to reduce immune elimination and enhance the stability of drugs in the bloodstream. In the present study, the potential of liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus (TelomeScan) expressing GFP (Lipo-pTS) as an oncolytic adenoviral agent suitable for systemic delivery was investigated. Lipo-pTS, which has a diameter of 40–50 nm, showed potent antitumor effects on HCT116 colon carcinoma cells in vitro and in vivo. Tumor selectivity of Lipo-pTS was independent of coxsackie and adenovirus receptor (CAR). Importantly, Lipo-pTS reduced production of adenovirus-neutralizing antibodies (AdNAbs) after intravenous administration into immune-competent mice compared to TelomeScan, and even in the presence of AdNAbs, Lipo-pTS maintained strong cytotoxicity. In conclusion, Lipo-pTS has the potential to become an oncolytic adenoviral agent suitable for systemic delivery with the characteristics of CAR-independent antitumor activity and a stealth effect on the immune system.
format	article
author	Katsuyuki Aoyama Shinji Kuroda Toshiaki Morihiro Nobuhiko Kanaya Tetsushi Kubota Yoshihiko Kakiuchi Satoru Kikuchi Masahiko Nishizaki Shunsuke Kagawa Hiroshi Tazawa Toshiyoshi Fujiwara
author_facet	Katsuyuki Aoyama Shinji Kuroda Toshiaki Morihiro Nobuhiko Kanaya Tetsushi Kubota Yoshihiko Kakiuchi Satoru Kikuchi Masahiko Nishizaki Shunsuke Kagawa Hiroshi Tazawa Toshiyoshi Fujiwara
author_sort	Katsuyuki Aoyama
title	Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
title_short	Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
title_full	Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
title_fullStr	Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
title_full_unstemmed	Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
title_sort	liposome-encapsulated plasmid dna of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/20760a868f17433c8d8ecd81b0ee0a17
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Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system

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