Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

Genetic imprinting ensures monoallelic gene expression critical for normal embryonic development. Here the authors take advantage of human haploid parthenogenic embryonic stem cells lacking paternal alleles to identify, by genome-wide screening, factors involved in the regulation of imprinted genes.

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Autores principales:	Shiran Bar, Dan Vershkov, Gal Keshet, Elyad Lezmi, Naama Meller, Atilgan Yilmaz, Ofra Yanuka, Malka Nissim-Rafinia, Eran Meshorer, Talia Eldar-Geva, Nissim Benvenisty
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/207e85234f0e4fce91d514642b759697
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spelling	oai:doaj.org-article:207e85234f0e4fce91d514642b7596972021-11-21T12:35:58ZIdentifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells10.1038/s41467-021-26949-72041-1723https://doaj.org/article/207e85234f0e4fce91d514642b7596972021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26949-7https://doaj.org/toc/2041-1723Genetic imprinting ensures monoallelic gene expression critical for normal embryonic development. Here the authors take advantage of human haploid parthenogenic embryonic stem cells lacking paternal alleles to identify, by genome-wide screening, factors involved in the regulation of imprinted genes.Shiran BarDan VershkovGal KeshetElyad LezmiNaama MellerAtilgan YilmazOfra YanukaMalka Nissim-RafiniaEran MeshorerTalia Eldar-GevaNissim BenvenistyNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shiran Bar Dan Vershkov Gal Keshet Elyad Lezmi Naama Meller Atilgan Yilmaz Ofra Yanuka Malka Nissim-Rafinia Eran Meshorer Talia Eldar-Geva Nissim Benvenisty Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
description	Genetic imprinting ensures monoallelic gene expression critical for normal embryonic development. Here the authors take advantage of human haploid parthenogenic embryonic stem cells lacking paternal alleles to identify, by genome-wide screening, factors involved in the regulation of imprinted genes.
format	article
author	Shiran Bar Dan Vershkov Gal Keshet Elyad Lezmi Naama Meller Atilgan Yilmaz Ofra Yanuka Malka Nissim-Rafinia Eran Meshorer Talia Eldar-Geva Nissim Benvenisty
author_facet	Shiran Bar Dan Vershkov Gal Keshet Elyad Lezmi Naama Meller Atilgan Yilmaz Ofra Yanuka Malka Nissim-Rafinia Eran Meshorer Talia Eldar-Geva Nissim Benvenisty
author_sort	Shiran Bar
title	Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
title_short	Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
title_full	Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
title_fullStr	Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
title_full_unstemmed	Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells
title_sort	identifying regulators of parental imprinting by crispr/cas9 screening in haploid human embryonic stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/207e85234f0e4fce91d514642b759697
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Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

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