MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors

Cuiyu Xin,1 Jiejing Xia,2 Yulan Liu,3 Yongdong Zhang4 1Department of Geriatric Psychiatry, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 2Department of Psychosis Ⅶ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, Peo...

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Autores principales:	Xin C, Xia J, Liu Y, Zhang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mir-202-3p bdnf creb1 depression hippocampus Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/20812a110b1145b3954d2d55a62658f8
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spelling	oai:doaj.org-article:20812a110b1145b3954d2d55a62658f82021-12-02T09:36:27ZMicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors1178-2021https://doaj.org/article/20812a110b1145b3954d2d55a62658f82020-04-01T00:00:00Zhttps://www.dovepress.com/microrna-202-3p-targets-brain-derived-neurotrophic-factor-and-is-invol-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Cuiyu Xin,1 Jiejing Xia,2 Yulan Liu,3 Yongdong Zhang4 1Department of Geriatric Psychiatry, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 2Department of Psychosis Ⅶ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 3Department of Psychosis Ⅴ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 4Department of Psychosis Ⅳ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of ChinaCorrespondence: Yongdong ZhangDepartment of Psychosis Ⅳ, Qingdao Mental Health Center, No. 299, Nanjing Road, Qingdao City, Shandong Province 266034, People’s Republic of ChinaTel +86-532-85621584Email mg2577@163.comBackground: Brain-derived neurotrophic factor (BDNF) and microRNA (miRNA) play crucial roles in the etiology of depression. However, the molecular mechanisms underlying this disease are not fully understood. The primary objective of this study was to investigate the relationship between miR-202-3p and BDNF in a chronic unpredictable mild stress (CUMS) model.Methods: Depression model was established with chronic mild unpredictable mild stimulation (CUMS) combined with solitary feeding. The expression levels of miR-202-3p and BDNF in rat hippocampus were measured by qRT-PCR. The novelty inhibition feeding test (NSFT), sucrose preference test (SPT), and forced swimming test (FST) were used to evaluate the functions of miR-202-3p and BDNF. Target gene prediction and screening and luciferase reporter assay were used to verify the target of miR-202-3p. The expression levels of BNDF, CREB1 and p-CREB1 were detected by Western blot.Results: Upregulation of miR-202-3p was associated with decreased expression of BDNF in the hippocampus of the CUMS model. Antidepressant was observed when LV-BDNF or LV-si-miR-202-3p was injected into the hippocampus. In addition, in the rat hippocampus and cultured nerve cells, the expression levels of BDNF and cyclic AMP response element binding protein 1 (CREB1), which is a target gene of BDNF, were reduced after LV-miR-202-3p injection. Overexpression of miR-202-3p aggravated depressive behavior and decreased the expression levels of BDNF. Luciferase reporter assay also confirmed that BDNF was a target of miR-202-3p.Conclusion: Silencing miR-202-3p can reduce the damage to hippocampal nerve in CUMS rats; the mechanism may be related to the upregulation of BNDF expression. miR-202-3p may be an effective target for the treatment of depression.Keywords: miR-202-3p, BDNF, CREB1, depression, hippocampusXin CXia JLiu YZhang YDove Medical Pressarticlemir-202-3pbdnfcreb1depressionhippocampusNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 1073-1083 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-202-3p bdnf creb1 depression hippocampus Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	mir-202-3p bdnf creb1 depression hippocampus Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Xin C Xia J Liu Y Zhang Y MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors
description	Cuiyu Xin,1 Jiejing Xia,2 Yulan Liu,3 Yongdong Zhang4 1Department of Geriatric Psychiatry, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 2Department of Psychosis Ⅶ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 3Department of Psychosis Ⅴ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of China; 4Department of Psychosis Ⅳ, Qingdao Mental Health Center, Qingdao City, Shandong Province 266034, People’s Republic of ChinaCorrespondence: Yongdong ZhangDepartment of Psychosis Ⅳ, Qingdao Mental Health Center, No. 299, Nanjing Road, Qingdao City, Shandong Province 266034, People’s Republic of ChinaTel +86-532-85621584Email mg2577@163.comBackground: Brain-derived neurotrophic factor (BDNF) and microRNA (miRNA) play crucial roles in the etiology of depression. However, the molecular mechanisms underlying this disease are not fully understood. The primary objective of this study was to investigate the relationship between miR-202-3p and BDNF in a chronic unpredictable mild stress (CUMS) model.Methods: Depression model was established with chronic mild unpredictable mild stimulation (CUMS) combined with solitary feeding. The expression levels of miR-202-3p and BDNF in rat hippocampus were measured by qRT-PCR. The novelty inhibition feeding test (NSFT), sucrose preference test (SPT), and forced swimming test (FST) were used to evaluate the functions of miR-202-3p and BDNF. Target gene prediction and screening and luciferase reporter assay were used to verify the target of miR-202-3p. The expression levels of BNDF, CREB1 and p-CREB1 were detected by Western blot.Results: Upregulation of miR-202-3p was associated with decreased expression of BDNF in the hippocampus of the CUMS model. Antidepressant was observed when LV-BDNF or LV-si-miR-202-3p was injected into the hippocampus. In addition, in the rat hippocampus and cultured nerve cells, the expression levels of BDNF and cyclic AMP response element binding protein 1 (CREB1), which is a target gene of BDNF, were reduced after LV-miR-202-3p injection. Overexpression of miR-202-3p aggravated depressive behavior and decreased the expression levels of BDNF. Luciferase reporter assay also confirmed that BDNF was a target of miR-202-3p.Conclusion: Silencing miR-202-3p can reduce the damage to hippocampal nerve in CUMS rats; the mechanism may be related to the upregulation of BNDF expression. miR-202-3p may be an effective target for the treatment of depression.Keywords: miR-202-3p, BDNF, CREB1, depression, hippocampus
format	article
author	Xin C Xia J Liu Y Zhang Y
author_facet	Xin C Xia J Liu Y Zhang Y
author_sort	Xin C
title	MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors
title_short	MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors
title_full	MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors
title_fullStr	MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors
title_full_unstemmed	MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors
title_sort	microrna-202-3p targets brain-derived neurotrophic factor and is involved in depression-like behaviors
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/20812a110b1145b3954d2d55a62658f8
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MicroRNA-202-3p Targets Brain-Derived Neurotrophic Factor and Is Involved in Depression-Like Behaviors

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