Image analysis for the quantitative comparison of stress fibers and focal adhesions.

Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins wit...

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Autores principales:	Alberto Elosegui-Artola, Alvaro Jorge-Peñas, Oihana Moreno-Arotzena, Amaia Oregi, Marta Lasa, José Manuel García-Aznar, Elena M De Juan-Pardo, Rafael Aldabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2085c717cb6a40b087b86edeb07f17c5
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spelling	oai:doaj.org-article:2085c717cb6a40b087b86edeb07f17c52021-11-25T05:58:26ZImage analysis for the quantitative comparison of stress fibers and focal adhesions.1932-620310.1371/journal.pone.0107393https://doaj.org/article/2085c717cb6a40b087b86edeb07f17c52014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107393https://doaj.org/toc/1932-6203Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.Alberto Elosegui-ArtolaAlvaro Jorge-PeñasOihana Moreno-ArotzenaAmaia OregiMarta LasaJosé Manuel García-AznarElena M De Juan-PardoRafael AldabePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107393 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alberto Elosegui-Artola Alvaro Jorge-Peñas Oihana Moreno-Arotzena Amaia Oregi Marta Lasa José Manuel García-Aznar Elena M De Juan-Pardo Rafael Aldabe Image analysis for the quantitative comparison of stress fibers and focal adhesions.
description	Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.
format	article
author	Alberto Elosegui-Artola Alvaro Jorge-Peñas Oihana Moreno-Arotzena Amaia Oregi Marta Lasa José Manuel García-Aznar Elena M De Juan-Pardo Rafael Aldabe
author_facet	Alberto Elosegui-Artola Alvaro Jorge-Peñas Oihana Moreno-Arotzena Amaia Oregi Marta Lasa José Manuel García-Aznar Elena M De Juan-Pardo Rafael Aldabe
author_sort	Alberto Elosegui-Artola
title	Image analysis for the quantitative comparison of stress fibers and focal adhesions.
title_short	Image analysis for the quantitative comparison of stress fibers and focal adhesions.
title_full	Image analysis for the quantitative comparison of stress fibers and focal adhesions.
title_fullStr	Image analysis for the quantitative comparison of stress fibers and focal adhesions.
title_full_unstemmed	Image analysis for the quantitative comparison of stress fibers and focal adhesions.
title_sort	image analysis for the quantitative comparison of stress fibers and focal adhesions.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2085c717cb6a40b087b86edeb07f17c5
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Image analysis for the quantitative comparison of stress fibers and focal adhesions.

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