Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser

Abstract Background Pulsed ultraviolet (UV)-C light sources, such as excimer lasers, are used in emerging non-thermal food-decontamination methods and also have high potential for use in a wide range of microbial decontamination applications. The acaricidal effect of an experimental UV-C irradiation...

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Autores principales:	Jean-Luc Gala, Ott Rebane, Jérôme Ambroise, Sergey Babichenko, Omar Nyabi, Thierry Hance
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Germicidal effect Physical control Ultraviolet-C Pulsed irradiation Excimer laser Krypton fluoride Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/208b8b668968411794e7679bf320aaa3
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spelling	oai:doaj.org-article:208b8b668968411794e7679bf320aaa32021-11-21T12:26:16ZAcaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser10.1186/s13071-021-05085-71756-3305https://doaj.org/article/208b8b668968411794e7679bf320aaa32021-11-01T00:00:00Zhttps://doi.org/10.1186/s13071-021-05085-7https://doaj.org/toc/1756-3305Abstract Background Pulsed ultraviolet (UV)-C light sources, such as excimer lasers, are used in emerging non-thermal food-decontamination methods and also have high potential for use in a wide range of microbial decontamination applications. The acaricidal effect of an experimental UV-C irradiation device was assessed using female adults and eggs of a model organism, the two-spotted spider mite Tetranychus urticae. Methods UV-C light was generated by a pulsed krypton fluoride excimer laser operating at 248-nm emission wavelength. The pulse energy and pulse repetition rate were 5 mJ and up to 100 Hz, respectively. The distance from the light source to the target was 150 mm; the target surface area was 2.16 cm2. The exposure time for the mites and fresh eggs varied from 1 to 4 min at 5–300 mW, which corresponded to UV doses of 5–80 kJ/m2. Post-irradiation acaricidal effects (mite mortality) were assessed immediately and also measured at 24 h. The effects of UV-C irradiation on the hatchability of eggs were observed daily for up to 12 days post-irradiation. Results The mortality of mites at 5 and 40 kJ/m2 was 26% and 92%, respectively. Mite mortality reached 98% at 80 kJ/m2. The effect of exposure duration on mortality was minimal. The effect of irradiation on egg hatchability was even more significant than that on adult mite mortality, i.e. about 100% egg mortality at an accumulated dose of as little as 5 kJ/m2 for each exposure time. Conclusions A high rate of mite mortality and lethal egg damage were observed after less than 1 min of exposure to 5 mJ UV-C pulsed irradiation at 60 Hz. Pending further developments (such as beam steering, beam shaping and miniaturisation) and feasibility studies (such as testing with mites in real-life situations), the reported results and characteristics of the UV-C generator (modulation of energy output and adaptability to varying spot sizes) open up the use of this technology for a vast field of acaricidal applications that require long-range radiation. Graphical AbstractJean-Luc GalaOtt RebaneJérôme AmbroiseSergey BabichenkoOmar NyabiThierry HanceBMCarticleGermicidal effectPhysical controlUltraviolet-CPulsed irradiationExcimer laserKrypton fluorideInfectious and parasitic diseasesRC109-216ENParasites & Vectors, Vol 14, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Germicidal effect Physical control Ultraviolet-C Pulsed irradiation Excimer laser Krypton fluoride Infectious and parasitic diseases RC109-216
spellingShingle	Germicidal effect Physical control Ultraviolet-C Pulsed irradiation Excimer laser Krypton fluoride Infectious and parasitic diseases RC109-216 Jean-Luc Gala Ott Rebane Jérôme Ambroise Sergey Babichenko Omar Nyabi Thierry Hance Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
description	Abstract Background Pulsed ultraviolet (UV)-C light sources, such as excimer lasers, are used in emerging non-thermal food-decontamination methods and also have high potential for use in a wide range of microbial decontamination applications. The acaricidal effect of an experimental UV-C irradiation device was assessed using female adults and eggs of a model organism, the two-spotted spider mite Tetranychus urticae. Methods UV-C light was generated by a pulsed krypton fluoride excimer laser operating at 248-nm emission wavelength. The pulse energy and pulse repetition rate were 5 mJ and up to 100 Hz, respectively. The distance from the light source to the target was 150 mm; the target surface area was 2.16 cm2. The exposure time for the mites and fresh eggs varied from 1 to 4 min at 5–300 mW, which corresponded to UV doses of 5–80 kJ/m2. Post-irradiation acaricidal effects (mite mortality) were assessed immediately and also measured at 24 h. The effects of UV-C irradiation on the hatchability of eggs were observed daily for up to 12 days post-irradiation. Results The mortality of mites at 5 and 40 kJ/m2 was 26% and 92%, respectively. Mite mortality reached 98% at 80 kJ/m2. The effect of exposure duration on mortality was minimal. The effect of irradiation on egg hatchability was even more significant than that on adult mite mortality, i.e. about 100% egg mortality at an accumulated dose of as little as 5 kJ/m2 for each exposure time. Conclusions A high rate of mite mortality and lethal egg damage were observed after less than 1 min of exposure to 5 mJ UV-C pulsed irradiation at 60 Hz. Pending further developments (such as beam steering, beam shaping and miniaturisation) and feasibility studies (such as testing with mites in real-life situations), the reported results and characteristics of the UV-C generator (modulation of energy output and adaptability to varying spot sizes) open up the use of this technology for a vast field of acaricidal applications that require long-range radiation. Graphical Abstract
format	article
author	Jean-Luc Gala Ott Rebane Jérôme Ambroise Sergey Babichenko Omar Nyabi Thierry Hance
author_facet	Jean-Luc Gala Ott Rebane Jérôme Ambroise Sergey Babichenko Omar Nyabi Thierry Hance
author_sort	Jean-Luc Gala
title	Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
title_short	Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
title_full	Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
title_fullStr	Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
title_full_unstemmed	Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
title_sort	acaricidal efficacy of ultraviolet-c irradiation of tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser
publisher	BMC
publishDate	2021
url	https://doaj.org/article/208b8b668968411794e7679bf320aaa3
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Acaricidal efficacy of ultraviolet-C irradiation of Tetranychus urticae adults and eggs using a pulsed krypton fluoride excimer laser

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