A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers

Abstract MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mikael Lenz Strube, Julie Elvekjær Hansen, Sophia Rasmussen, Karl Pedersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/208d1ace133f4cb1befe1eca652105ed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:208d1ace133f4cb1befe1eca652105ed
record_format	dspace
spelling	oai:doaj.org-article:208d1ace133f4cb1befe1eca652105ed2021-12-02T15:07:58ZA detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers10.1038/s41598-018-30689-y2045-2322https://doaj.org/article/208d1ace133f4cb1befe1eca652105ed2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30689-yhttps://doaj.org/toc/2045-2322Abstract MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.Mikael Lenz StrubeJulie Elvekjær HansenSophia RasmussenKarl PedersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mikael Lenz Strube Julie Elvekjær Hansen Sophia Rasmussen Karl Pedersen A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
description	Abstract MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.
format	article
author	Mikael Lenz Strube Julie Elvekjær Hansen Sophia Rasmussen Karl Pedersen
author_facet	Mikael Lenz Strube Julie Elvekjær Hansen Sophia Rasmussen Karl Pedersen
author_sort	Mikael Lenz Strube
title	A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
title_short	A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
title_full	A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
title_fullStr	A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
title_full_unstemmed	A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
title_sort	detailed investigation of the porcine skin and nose microbiome using universal and staphylococcus specific primers
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/208d1ace133f4cb1befe1eca652105ed
work_keys_str_mv	AT mikaellenzstrube adetailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT julieelvekjærhansen adetailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT sophiarasmussen adetailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT karlpedersen adetailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT mikaellenzstrube detailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT julieelvekjærhansen detailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT sophiarasmussen detailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers AT karlpedersen detailedinvestigationoftheporcineskinandnosemicrobiomeusinguniversalandstaphylococcusspecificprimers
_version_	1718388303257927680

A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers

Ejemplares similares