Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.

In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 comple...

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Autores principales:	Letícia Christina Pires Gonçalves, Sandra Maria Da Silva, Paul C DeRose, Rômulo Augusto Ando, Erick Leite Bastos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2094e14deea3450fbf9b7c63e9ab0474
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spelling	oai:doaj.org-article:2094e14deea3450fbf9b7c63e9ab04742021-11-18T08:57:10ZBeetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.1932-620310.1371/journal.pone.0073701https://doaj.org/article/2094e14deea3450fbf9b7c63e9ab04742013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24019934/?tool=EBIhttps://doaj.org/toc/1932-6203In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 complex with a stability constant of 1.4 × 10(5) L mol(-1). The addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of [Eu(Bn)(+)] from orange to magenta. The limit of detection (LOD) of calcium dipicolinate is around 2.0 × 10(-6) mol L(-1) and the LOD determined for both spores, B. cereus and B. anthracis, is (1.1 ± 0.3)× 10(6) spores mL(-1). This simple, green, fast and low cost colorimetric assay was selective for calcium dipicolinate when compared to several analogous compounds. The importance of this work relies on the potential use of betalains, raw natural pigments, as colorimetric sensors for biological applications.Letícia Christina Pires GonçalvesSandra Maria Da SilvaPaul C DeRoseRômulo Augusto AndoErick Leite BastosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73701 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Letícia Christina Pires Gonçalves Sandra Maria Da Silva Paul C DeRose Rômulo Augusto Ando Erick Leite Bastos Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
description	In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 complex with a stability constant of 1.4 × 10(5) L mol(-1). The addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of [Eu(Bn)(+)] from orange to magenta. The limit of detection (LOD) of calcium dipicolinate is around 2.0 × 10(-6) mol L(-1) and the LOD determined for both spores, B. cereus and B. anthracis, is (1.1 ± 0.3)× 10(6) spores mL(-1). This simple, green, fast and low cost colorimetric assay was selective for calcium dipicolinate when compared to several analogous compounds. The importance of this work relies on the potential use of betalains, raw natural pigments, as colorimetric sensors for biological applications.
format	article
author	Letícia Christina Pires Gonçalves Sandra Maria Da Silva Paul C DeRose Rômulo Augusto Ando Erick Leite Bastos
author_facet	Letícia Christina Pires Gonçalves Sandra Maria Da Silva Paul C DeRose Rômulo Augusto Ando Erick Leite Bastos
author_sort	Letícia Christina Pires Gonçalves
title	Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
title_short	Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
title_full	Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
title_fullStr	Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
title_full_unstemmed	Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
title_sort	beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2094e14deea3450fbf9b7c63e9ab0474
work_keys_str_mv	AT leticiachristinapiresgoncalves beetrootpigmentderivedcolorimetricsensorfordetectionofcalciumdipicolinateinbacterialspores AT sandramariadasilva beetrootpigmentderivedcolorimetricsensorfordetectionofcalciumdipicolinateinbacterialspores AT paulcderose beetrootpigmentderivedcolorimetricsensorfordetectionofcalciumdipicolinateinbacterialspores AT romuloaugustoando beetrootpigmentderivedcolorimetricsensorfordetectionofcalciumdipicolinateinbacterialspores AT erickleitebastos beetrootpigmentderivedcolorimetricsensorfordetectionofcalciumdipicolinateinbacterialspores
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Beetroot-pigment-derived colorimetric sensor for detection of calcium dipicolinate in bacterial spores.

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