Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI

Abstract Several studies have suggested that extracellular matrix (ECM) remodeling and the microenvironment are tightly associated with adipogenesis and adipose angiogenesis. In the present study, we demonstrated that transforming growth factor-beta induced (TGFBI) suppresses angiogenesis stimulated...

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Autores principales:	Seul Gi Lee, Jin Soo Kim, Ha-Jeong Kim, David D. Schlaepfer, In-San Kim, Ju-Ock Nam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20961660dfa44eeeb187021fed404e74
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spelling	oai:doaj.org-article:20961660dfa44eeeb187021fed404e742021-12-02T14:49:25ZEndothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI10.1038/s41598-021-88959-12045-2322https://doaj.org/article/20961660dfa44eeeb187021fed404e742021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88959-1https://doaj.org/toc/2045-2322Abstract Several studies have suggested that extracellular matrix (ECM) remodeling and the microenvironment are tightly associated with adipogenesis and adipose angiogenesis. In the present study, we demonstrated that transforming growth factor-beta induced (TGFBI) suppresses angiogenesis stimulated by adipocyte-conditioned medium (Ad-CM), both in vitro and in vivo. TGFBI knockout (KO) mice exhibited increased numbers of blood vessels in adipose tissue, and blood vessels from these mice showed enhanced infiltration into Matrigel containing Ad-CM. The treatment of Ad-CM-stimulated SVEC-10 endothelial cells with TGFBI protein reduced migration and tube-forming activity. TGFBI protein suppressed the activation of the Src and extracellular signaling-related kinase signaling pathways of these SVEC-10 endothelial cells. Our findings indicated that TGFBI inhibited adipose angiogenesis by suppressing the activation of Src and ERK signaling pathways, possibly because of the stimulation of the angiogenic activity of endothelial cells.Seul Gi LeeJin Soo KimHa-Jeong KimDavid D. SchlaepferIn-San KimJu-Ock NamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seul Gi Lee Jin Soo Kim Ha-Jeong Kim David D. Schlaepfer In-San Kim Ju-Ock Nam Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI
description	Abstract Several studies have suggested that extracellular matrix (ECM) remodeling and the microenvironment are tightly associated with adipogenesis and adipose angiogenesis. In the present study, we demonstrated that transforming growth factor-beta induced (TGFBI) suppresses angiogenesis stimulated by adipocyte-conditioned medium (Ad-CM), both in vitro and in vivo. TGFBI knockout (KO) mice exhibited increased numbers of blood vessels in adipose tissue, and blood vessels from these mice showed enhanced infiltration into Matrigel containing Ad-CM. The treatment of Ad-CM-stimulated SVEC-10 endothelial cells with TGFBI protein reduced migration and tube-forming activity. TGFBI protein suppressed the activation of the Src and extracellular signaling-related kinase signaling pathways of these SVEC-10 endothelial cells. Our findings indicated that TGFBI inhibited adipose angiogenesis by suppressing the activation of Src and ERK signaling pathways, possibly because of the stimulation of the angiogenic activity of endothelial cells.
format	article
author	Seul Gi Lee Jin Soo Kim Ha-Jeong Kim David D. Schlaepfer In-San Kim Ju-Ock Nam
author_facet	Seul Gi Lee Jin Soo Kim Ha-Jeong Kim David D. Schlaepfer In-San Kim Ju-Ock Nam
author_sort	Seul Gi Lee
title	Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI
title_short	Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI
title_full	Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI
title_fullStr	Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI
title_full_unstemmed	Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI
title_sort	endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein tgfbi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/20961660dfa44eeeb187021fed404e74
work_keys_str_mv	AT seulgilee endothelialangiogenicactivityandadiposeangiogenesisiscontrolledbyextracellularmatrixproteintgfbi AT jinsookim endothelialangiogenicactivityandadiposeangiogenesisiscontrolledbyextracellularmatrixproteintgfbi AT hajeongkim endothelialangiogenicactivityandadiposeangiogenesisiscontrolledbyextracellularmatrixproteintgfbi AT daviddschlaepfer endothelialangiogenicactivityandadiposeangiogenesisiscontrolledbyextracellularmatrixproteintgfbi AT insankim endothelialangiogenicactivityandadiposeangiogenesisiscontrolledbyextracellularmatrixproteintgfbi AT juocknam endothelialangiogenicactivityandadiposeangiogenesisiscontrolledbyextracellularmatrixproteintgfbi
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Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI

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