Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods

Mohankandhasamy Ramasamy,1 Dong Kee Yi,2,3 Seong Soo A An4 1School of Chemical Engineering, Yeungnam University, Gyeongsan, 2Department of Chemistry, 3Department of Energy and Biotechnology, Myongji University, Yongin, 4Department of BioNano Technology, Gachon University, Seongnam, Republic of Kore...

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Autores principales:	Ramasamy M, Yi DK, An SSA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/209bd6c706e140eba453227514061ebf
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spelling	oai:doaj.org-article:209bd6c706e140eba453227514061ebf2021-12-02T05:52:53ZEnhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods1178-2013https://doaj.org/article/209bd6c706e140eba453227514061ebf2015-08-01T00:00:00Zhttp://www.dovepress.com/enhanced-detection-sensitivity-of-escherichia-coli-o157h7-using-surfac-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mohankandhasamy Ramasamy,1 Dong Kee Yi,2,3 Seong Soo A An4 1School of Chemical Engineering, Yeungnam University, Gyeongsan, 2Department of Chemistry, 3Department of Energy and Biotechnology, Myongji University, Yongin, 4Department of BioNano Technology, Gachon University, Seongnam, Republic of Korea Abstract: Escherichia coli O157:H7 (O157) is a Gram negative and highly virulent bacteria found in food and water sources, and is a leading cause of chronic diseases worldwide. Diagnosis and prevention from the infection require simple and rapid analysis methods for the detection of pathogens, including O157. Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate. In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157. Along with the membrane peroxidase activity of O157, other bacteria strains were analyzed. Different concentrations of nanorods were used to analyze the growth responses, enzymatic changes, and morphological alterations of bacteria by measuring optical density, 3,3',5,5'-tetramethylbenzidine assay, flow cytometry analysis, and microscopy studies. The results revealed that O157 showed higher and continuous membrane peroxidase activity than other bacteria. Furthermore, O157 treated with bare AuNRs showed a decreased growth rate in comparison with the bacteria with surface modified AuNRs. Interestingly, silica-coated AuNRs favored the growth of bacteria and also increased membrane peroxidase activity. This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants. Keywords: E. coli, O157:H7, endogenous membrane peroxidase, gold nanorod, silica-coated gold nanorodRamasamy MYi DKAn SSADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 179-190 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ramasamy M Yi DK An SSA Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods
description	Mohankandhasamy Ramasamy,1 Dong Kee Yi,2,3 Seong Soo A An4 1School of Chemical Engineering, Yeungnam University, Gyeongsan, 2Department of Chemistry, 3Department of Energy and Biotechnology, Myongji University, Yongin, 4Department of BioNano Technology, Gachon University, Seongnam, Republic of Korea Abstract: Escherichia coli O157:H7 (O157) is a Gram negative and highly virulent bacteria found in food and water sources, and is a leading cause of chronic diseases worldwide. Diagnosis and prevention from the infection require simple and rapid analysis methods for the detection of pathogens, including O157. Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate. In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157. Along with the membrane peroxidase activity of O157, other bacteria strains were analyzed. Different concentrations of nanorods were used to analyze the growth responses, enzymatic changes, and morphological alterations of bacteria by measuring optical density, 3,3',5,5'-tetramethylbenzidine assay, flow cytometry analysis, and microscopy studies. The results revealed that O157 showed higher and continuous membrane peroxidase activity than other bacteria. Furthermore, O157 treated with bare AuNRs showed a decreased growth rate in comparison with the bacteria with surface modified AuNRs. Interestingly, silica-coated AuNRs favored the growth of bacteria and also increased membrane peroxidase activity. This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants. Keywords: E. coli, O157:H7, endogenous membrane peroxidase, gold nanorod, silica-coated gold nanorod
format	article
author	Ramasamy M Yi DK An SSA
author_facet	Ramasamy M Yi DK An SSA
author_sort	Ramasamy M
title	Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods
title_short	Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods
title_full	Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods
title_fullStr	Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods
title_full_unstemmed	Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods
title_sort	enhanced detection sensitivity of escherichia coli o157:h7 using surface-modified gold nanorods
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/209bd6c706e140eba453227514061ebf
work_keys_str_mv	AT ramasamym enhanceddetectionsensitivityofescherichiacolio157h7usingsurfacemodifiedgoldnanorods AT yidk enhanceddetectionsensitivityofescherichiacolio157h7usingsurfacemodifiedgoldnanorods AT anssa enhanceddetectionsensitivityofescherichiacolio157h7usingsurfacemodifiedgoldnanorods
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Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods

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