A novel role of RASSF9 in maintaining epidermal homeostasis.

The physiological role of RASSF9, a member of the Ras-association domain family (RASSF), is currently unclear. Here, we report a mouse line in which an Epstein-Barr virus Latent Membrane Protein 1 (LMP1) transgene insertion has created a 7.2-kb chromosomal deletion, which abolished RASSF9 gene expre...

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Autores principales:	Chiou-Mei Lee, Polung Yang, Lih-Chyang Chen, Chia-Chun Chen, Shinn-Chih Wu, Hsiao-Yun Cheng, Yu-Sun Chang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/209e383b242947dcb20e78ae15880c76
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spelling	oai:doaj.org-article:209e383b242947dcb20e78ae15880c762021-11-18T06:57:03ZA novel role of RASSF9 in maintaining epidermal homeostasis.1932-620310.1371/journal.pone.0017867https://doaj.org/article/209e383b242947dcb20e78ae15880c762011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21445300/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The physiological role of RASSF9, a member of the Ras-association domain family (RASSF), is currently unclear. Here, we report a mouse line in which an Epstein-Barr virus Latent Membrane Protein 1 (LMP1) transgene insertion has created a 7.2-kb chromosomal deletion, which abolished RASSF9 gene expression. The RASSF9-null mice exhibited interesting phenotypes that resembled human ageing, including growth retardation, short lifespan, less subcutaneous adipose layer and alopecia. In the wild-type mice, RASSF9 is predominantly expressed in the epidermal keratinocytes of skin, as determined by quantitative reverse-transcription PCR, immunofluorescence and in situ hybridization. In contrast, RASSF9-/- mice presented a dramatic change in epithelial organization of skin with increased proliferation and aberrant differentiation as detected by bromodeoxyuridine incorporation assays and immunofluorescence analyses. Furthermore, characteristic functions of RASSF9-/- versus wild type (WT) mouse primary keratinocytes showed significant proliferation linked to a reduction of p21Cip1 expression under growth or early differentiation conditions. Additionally, in RASSF9-/- keratinocytes there was a drastic down-modulation of terminal differentiation markers, which could be rescued by infection with a recombinant adenovirus, Adv/HA-RASSF9. Our results indicate a novel and significant role of RASSF9 in epidermal homeostasis.Chiou-Mei LeePolung YangLih-Chyang ChenChia-Chun ChenShinn-Chih WuHsiao-Yun ChengYu-Sun ChangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17867 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chiou-Mei Lee Polung Yang Lih-Chyang Chen Chia-Chun Chen Shinn-Chih Wu Hsiao-Yun Cheng Yu-Sun Chang A novel role of RASSF9 in maintaining epidermal homeostasis.
description	The physiological role of RASSF9, a member of the Ras-association domain family (RASSF), is currently unclear. Here, we report a mouse line in which an Epstein-Barr virus Latent Membrane Protein 1 (LMP1) transgene insertion has created a 7.2-kb chromosomal deletion, which abolished RASSF9 gene expression. The RASSF9-null mice exhibited interesting phenotypes that resembled human ageing, including growth retardation, short lifespan, less subcutaneous adipose layer and alopecia. In the wild-type mice, RASSF9 is predominantly expressed in the epidermal keratinocytes of skin, as determined by quantitative reverse-transcription PCR, immunofluorescence and in situ hybridization. In contrast, RASSF9-/- mice presented a dramatic change in epithelial organization of skin with increased proliferation and aberrant differentiation as detected by bromodeoxyuridine incorporation assays and immunofluorescence analyses. Furthermore, characteristic functions of RASSF9-/- versus wild type (WT) mouse primary keratinocytes showed significant proliferation linked to a reduction of p21Cip1 expression under growth or early differentiation conditions. Additionally, in RASSF9-/- keratinocytes there was a drastic down-modulation of terminal differentiation markers, which could be rescued by infection with a recombinant adenovirus, Adv/HA-RASSF9. Our results indicate a novel and significant role of RASSF9 in epidermal homeostasis.
format	article
author	Chiou-Mei Lee Polung Yang Lih-Chyang Chen Chia-Chun Chen Shinn-Chih Wu Hsiao-Yun Cheng Yu-Sun Chang
author_facet	Chiou-Mei Lee Polung Yang Lih-Chyang Chen Chia-Chun Chen Shinn-Chih Wu Hsiao-Yun Cheng Yu-Sun Chang
author_sort	Chiou-Mei Lee
title	A novel role of RASSF9 in maintaining epidermal homeostasis.
title_short	A novel role of RASSF9 in maintaining epidermal homeostasis.
title_full	A novel role of RASSF9 in maintaining epidermal homeostasis.
title_fullStr	A novel role of RASSF9 in maintaining epidermal homeostasis.
title_full_unstemmed	A novel role of RASSF9 in maintaining epidermal homeostasis.
title_sort	novel role of rassf9 in maintaining epidermal homeostasis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/209e383b242947dcb20e78ae15880c76
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A novel role of RASSF9 in maintaining epidermal homeostasis.

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