Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans

Abstract The ability of mycoplasmas to persist on surfaces has been widely acknowledged, despite their fastidious nature. However, the organism’s capability to form a recognisable biofilm structure has been identified more recently. In the current study Mycoplasma fermentans was found to adhere to t...

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Autores principales:	Ammar A. Awadh, Alison F. Kelly, Gary Forster-Wilkins, David Wertheim, Richard Giddens, Simon W. Gould, Mark D. Fielder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20a02dbbee494f388ce113907d6167ef
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spelling	oai:doaj.org-article:20a02dbbee494f388ce113907d6167ef2021-12-02T15:49:42ZVisualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans10.1038/s41598-021-90455-52045-2322https://doaj.org/article/20a02dbbee494f388ce113907d6167ef2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90455-5https://doaj.org/toc/2045-2322Abstract The ability of mycoplasmas to persist on surfaces has been widely acknowledged, despite their fastidious nature. However, the organism’s capability to form a recognisable biofilm structure has been identified more recently. In the current study Mycoplasma fermentans was found to adhere to the glass surface forming highly differentiated biofilm structures. The volumes of biofilm microcolonies were quantified and observed to be greater at late growth stage than those at early growth stage. The channel diameters within biofilms were measured with Scanning Electron Microscopy images and found to be consistent with the size observed in Confocal Laser Scanning Microscope images. The combination of imaging methods with 3D visualisation provides key findings that aid understanding of the mycoplasma biofilm formation and true biofilm architecture. The observations reported here provide better understanding of the persistence of these minimalist pathogens in nature and clinical settings.Ammar A. AwadhAlison F. KellyGary Forster-WilkinsDavid WertheimRichard GiddensSimon W. GouldMark D. FielderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ammar A. Awadh Alison F. Kelly Gary Forster-Wilkins David Wertheim Richard Giddens Simon W. Gould Mark D. Fielder Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans
description	Abstract The ability of mycoplasmas to persist on surfaces has been widely acknowledged, despite their fastidious nature. However, the organism’s capability to form a recognisable biofilm structure has been identified more recently. In the current study Mycoplasma fermentans was found to adhere to the glass surface forming highly differentiated biofilm structures. The volumes of biofilm microcolonies were quantified and observed to be greater at late growth stage than those at early growth stage. The channel diameters within biofilms were measured with Scanning Electron Microscopy images and found to be consistent with the size observed in Confocal Laser Scanning Microscope images. The combination of imaging methods with 3D visualisation provides key findings that aid understanding of the mycoplasma biofilm formation and true biofilm architecture. The observations reported here provide better understanding of the persistence of these minimalist pathogens in nature and clinical settings.
format	article
author	Ammar A. Awadh Alison F. Kelly Gary Forster-Wilkins David Wertheim Richard Giddens Simon W. Gould Mark D. Fielder
author_facet	Ammar A. Awadh Alison F. Kelly Gary Forster-Wilkins David Wertheim Richard Giddens Simon W. Gould Mark D. Fielder
author_sort	Ammar A. Awadh
title	Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans
title_short	Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans
title_full	Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans
title_fullStr	Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans
title_full_unstemmed	Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans
title_sort	visualisation and biovolume quantification in the characterisation of biofilm formation in mycoplasma fermentans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/20a02dbbee494f388ce113907d6167ef
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Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans

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