Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles

TH Nguyen Pham,1 Nigel A Lengkeek,2 Ivan Greguric,2 Byung J Kim,1 Paul A Pellegrini,2 Stephanie A Bickley,3 Marcel R Tanudji,3 Stephen K Jones,3 Brian S Hawkett,1 Binh TT Pham1 1Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, 2Radioisotopes and Radiotracers, NSTLI,...

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Autores principales:	Pham THN, Lengkeek NA, Greguric I, Kim BJ, Pellegrini PA, Bickley SA, Tanudji MR, Jones SK, Hawkett BS, Pham BTT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	multimodality imaging probe magnetic resonance imaging (MRI) positron emission tomography (PET) single-photon emission computed tomography (SPECT) superparamagnetic iron oxide nanoparticles (SPIONs) radiolabeling Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/20a0dc6990584048899dcbe0f2caa29c
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spelling	oai:doaj.org-article:20a0dc6990584048899dcbe0f2caa29c2021-12-02T01:50:41ZTunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles1178-2013https://doaj.org/article/20a0dc6990584048899dcbe0f2caa29c2017-01-01T00:00:00Zhttps://www.dovepress.com/tunable-and-noncytotoxic-petspect-mri-multimodality-imaging-probes-usi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013TH Nguyen Pham,1 Nigel A Lengkeek,2 Ivan Greguric,2 Byung J Kim,1 Paul A Pellegrini,2 Stephanie A Bickley,3 Marcel R Tanudji,3 Stephen K Jones,3 Brian S Hawkett,1 Binh TT Pham1 1Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, 2Radioisotopes and Radiotracers, NSTLI, Australian Nuclear Science and Technology Organisation, Sydney, 3Sirtex Medical Limited, North Sydney, NSW, Australia Abstract: Physiologically stable multimodality imaging probes for positron emission tomography/single-photon emission computed tomography (PET/SPECT)-magnetic resonance imaging (MRI) were synthesized using the superparamagnetic maghemite iron oxide (γ-Fe2O3) nanoparticles (SPIONs). The SPIONs were sterically stabilized with a finely tuned mixture of diblock copolymers with either methoxypolyethylene glycol (MPEG) or primary amine NH2 end groups. The radioisotope for PET or SPECT imaging was incorporated with the SPIONs at high temperature. 57Co2+ ions with a long half-life of 270.9 days were used as a model for the radiotracer to study the kinetics of radiolabeling, characterization, and the stability of the radiolabeled SPIONs. Radioactive 67Ga3+ and Cu2+-labeled SPIONs were also produced successfully using the optimized conditions from the 57Co2+-labeling process. No free radioisotopes were detected in the aqueous phase for the radiolabeled SPIONs 1 week after dispersion in phosphate-buffered saline (PBS). All labeled SPIONs were not only well dispersed and stable under physiological conditions but also noncytotoxic in vitro. The ability to design and produce physiologically stable radiolabeled magnetic nanoparticles with a finely controlled number of functionalizable end groups on the SPIONs enables the generation of a desirable and biologically compatible multimodality PET/SPECT-MRI agent on a single T2 contrast MRI probe. Keywords: magnetic resonance imaging, positron emission tomography, single-photon emission computed tomography, SPIONs, radiolabelingPham THNLengkeek NAGreguric IKim BJPellegrini PABickley SATanudji MRJones SKHawkett BSPham BTTDove Medical Pressarticlemultimodality imaging probemagnetic resonance imaging (MRI)positron emission tomography (PET)single-photon emission computed tomography (SPECT)superparamagnetic iron oxide nanoparticles (SPIONs)radiolabelingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 899-909 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	multimodality imaging probe magnetic resonance imaging (MRI) positron emission tomography (PET) single-photon emission computed tomography (SPECT) superparamagnetic iron oxide nanoparticles (SPIONs) radiolabeling Medicine (General) R5-920
spellingShingle	multimodality imaging probe magnetic resonance imaging (MRI) positron emission tomography (PET) single-photon emission computed tomography (SPECT) superparamagnetic iron oxide nanoparticles (SPIONs) radiolabeling Medicine (General) R5-920 Pham THN Lengkeek NA Greguric I Kim BJ Pellegrini PA Bickley SA Tanudji MR Jones SK Hawkett BS Pham BTT Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
description	TH Nguyen Pham,1 Nigel A Lengkeek,2 Ivan Greguric,2 Byung J Kim,1 Paul A Pellegrini,2 Stephanie A Bickley,3 Marcel R Tanudji,3 Stephen K Jones,3 Brian S Hawkett,1 Binh TT Pham1 1Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, 2Radioisotopes and Radiotracers, NSTLI, Australian Nuclear Science and Technology Organisation, Sydney, 3Sirtex Medical Limited, North Sydney, NSW, Australia Abstract: Physiologically stable multimodality imaging probes for positron emission tomography/single-photon emission computed tomography (PET/SPECT)-magnetic resonance imaging (MRI) were synthesized using the superparamagnetic maghemite iron oxide (γ-Fe2O3) nanoparticles (SPIONs). The SPIONs were sterically stabilized with a finely tuned mixture of diblock copolymers with either methoxypolyethylene glycol (MPEG) or primary amine NH2 end groups. The radioisotope for PET or SPECT imaging was incorporated with the SPIONs at high temperature. 57Co2+ ions with a long half-life of 270.9 days were used as a model for the radiotracer to study the kinetics of radiolabeling, characterization, and the stability of the radiolabeled SPIONs. Radioactive 67Ga3+ and Cu2+-labeled SPIONs were also produced successfully using the optimized conditions from the 57Co2+-labeling process. No free radioisotopes were detected in the aqueous phase for the radiolabeled SPIONs 1 week after dispersion in phosphate-buffered saline (PBS). All labeled SPIONs were not only well dispersed and stable under physiological conditions but also noncytotoxic in vitro. The ability to design and produce physiologically stable radiolabeled magnetic nanoparticles with a finely controlled number of functionalizable end groups on the SPIONs enables the generation of a desirable and biologically compatible multimodality PET/SPECT-MRI agent on a single T2 contrast MRI probe. Keywords: magnetic resonance imaging, positron emission tomography, single-photon emission computed tomography, SPIONs, radiolabeling
format	article
author	Pham THN Lengkeek NA Greguric I Kim BJ Pellegrini PA Bickley SA Tanudji MR Jones SK Hawkett BS Pham BTT
author_facet	Pham THN Lengkeek NA Greguric I Kim BJ Pellegrini PA Bickley SA Tanudji MR Jones SK Hawkett BS Pham BTT
author_sort	Pham THN
title	Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
title_short	Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
title_full	Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
title_fullStr	Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
title_full_unstemmed	Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
title_sort	tunable and noncytotoxic pet/spect-mri multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/20a0dc6990584048899dcbe0f2caa29c
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_version_	1718402820012507136

Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles

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