Inhibition of enveloped viruses infectivity by curcumin.

Curcumin, a natural compound and ingredient in curry, has antiinflammatory, antioxidant, and anticarcinogenic properties. Previously, we reported that curcumin abrogated influenza virus infectivity by inhibiting hemagglutination (HA) activity. This study demonstrates a novel mechanism by which curcu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tzu-Yen Chen, Da-Yuan Chen, Hsiao-Wei Wen, Jun-Lin Ou, Shyan-Song Chiou, Jo-Mei Chen, Min-Liang Wong, Wei-Li Hsu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20a29772f3134e8aa22048eb11242cbc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:20a29772f3134e8aa22048eb11242cbc
record_format	dspace
spelling	oai:doaj.org-article:20a29772f3134e8aa22048eb11242cbc2021-11-18T07:47:07ZInhibition of enveloped viruses infectivity by curcumin.1932-620310.1371/journal.pone.0062482https://doaj.org/article/20a29772f3134e8aa22048eb11242cbc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23658730/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Curcumin, a natural compound and ingredient in curry, has antiinflammatory, antioxidant, and anticarcinogenic properties. Previously, we reported that curcumin abrogated influenza virus infectivity by inhibiting hemagglutination (HA) activity. This study demonstrates a novel mechanism by which curcumin inhibits the infectivity of enveloped viruses. In all analyzed enveloped viruses, including the influenza virus, curcumin inhibited plaque formation. In contrast, the nonenveloped enterovirus 71 remained unaffected by curcumin treatment. We evaluated the effects of curcumin on the membrane structure using fluorescent dye (sulforhodamine B; SRB)-containing liposomes that mimic the viral envelope. Curcumin treatment induced the leakage of SRB from these liposomes and the addition of the influenza virus reduced the leakage, indicating that curcumin disrupts the integrity of the membranes of viral envelopes and of liposomes. When testing liposomes of various diameters, we detected higher levels of SRB leakage from the smaller-sized liposomes than from the larger liposomes. Interestingly, the curcumin concentration required to reduce plaque formation was lower for the influenza virus (approximately 100 nm in diameter) than for the pseudorabies virus (approximately 180 nm) and the vaccinia virus (roughly 335 × 200 × 200 nm). These data provide insights on the molecular antiviral mechanisms of curcumin and its potential use as an antiviral agent for enveloped viruses.Tzu-Yen ChenDa-Yuan ChenHsiao-Wei WenJun-Lin OuShyan-Song ChiouJo-Mei ChenMin-Liang WongWei-Li HsuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62482 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tzu-Yen Chen Da-Yuan Chen Hsiao-Wei Wen Jun-Lin Ou Shyan-Song Chiou Jo-Mei Chen Min-Liang Wong Wei-Li Hsu Inhibition of enveloped viruses infectivity by curcumin.
description	Curcumin, a natural compound and ingredient in curry, has antiinflammatory, antioxidant, and anticarcinogenic properties. Previously, we reported that curcumin abrogated influenza virus infectivity by inhibiting hemagglutination (HA) activity. This study demonstrates a novel mechanism by which curcumin inhibits the infectivity of enveloped viruses. In all analyzed enveloped viruses, including the influenza virus, curcumin inhibited plaque formation. In contrast, the nonenveloped enterovirus 71 remained unaffected by curcumin treatment. We evaluated the effects of curcumin on the membrane structure using fluorescent dye (sulforhodamine B; SRB)-containing liposomes that mimic the viral envelope. Curcumin treatment induced the leakage of SRB from these liposomes and the addition of the influenza virus reduced the leakage, indicating that curcumin disrupts the integrity of the membranes of viral envelopes and of liposomes. When testing liposomes of various diameters, we detected higher levels of SRB leakage from the smaller-sized liposomes than from the larger liposomes. Interestingly, the curcumin concentration required to reduce plaque formation was lower for the influenza virus (approximately 100 nm in diameter) than for the pseudorabies virus (approximately 180 nm) and the vaccinia virus (roughly 335 × 200 × 200 nm). These data provide insights on the molecular antiviral mechanisms of curcumin and its potential use as an antiviral agent for enveloped viruses.
format	article
author	Tzu-Yen Chen Da-Yuan Chen Hsiao-Wei Wen Jun-Lin Ou Shyan-Song Chiou Jo-Mei Chen Min-Liang Wong Wei-Li Hsu
author_facet	Tzu-Yen Chen Da-Yuan Chen Hsiao-Wei Wen Jun-Lin Ou Shyan-Song Chiou Jo-Mei Chen Min-Liang Wong Wei-Li Hsu
author_sort	Tzu-Yen Chen
title	Inhibition of enveloped viruses infectivity by curcumin.
title_short	Inhibition of enveloped viruses infectivity by curcumin.
title_full	Inhibition of enveloped viruses infectivity by curcumin.
title_fullStr	Inhibition of enveloped viruses infectivity by curcumin.
title_full_unstemmed	Inhibition of enveloped viruses infectivity by curcumin.
title_sort	inhibition of enveloped viruses infectivity by curcumin.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/20a29772f3134e8aa22048eb11242cbc
work_keys_str_mv	AT tzuyenchen inhibitionofenvelopedvirusesinfectivitybycurcumin AT dayuanchen inhibitionofenvelopedvirusesinfectivitybycurcumin AT hsiaoweiwen inhibitionofenvelopedvirusesinfectivitybycurcumin AT junlinou inhibitionofenvelopedvirusesinfectivitybycurcumin AT shyansongchiou inhibitionofenvelopedvirusesinfectivitybycurcumin AT jomeichen inhibitionofenvelopedvirusesinfectivitybycurcumin AT minliangwong inhibitionofenvelopedvirusesinfectivitybycurcumin AT weilihsu inhibitionofenvelopedvirusesinfectivitybycurcumin
_version_	1718422958809022464

Inhibition of enveloped viruses infectivity by curcumin.

Ejemplares similares