The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling

Worldwide prostate cancer is the second leading cause of cancer death among men after lung cancer. MicroRNAs are non-coding, endogenous RNAs and they play a role in tumorigenesis, RNA silencing and post-transcriptional regulation of gene expression. In this study we have investigated microRNA-15a im...

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Autores principales:	Apostolova A., Sezer L., Hayrabedyan S., Todorova Kr.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	microrna-15a prostate cancer transcription factors cmyb ets1 nfκb Medicine R
Acceso en línea:	https://doaj.org/article/20a85ae018a445ceaab58f206287dfd6
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spelling	oai:doaj.org-article:20a85ae018a445ceaab58f206287dfd62021-12-02T14:59:50ZThe Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling0324-175010.2478/amb-2018-0014https://doaj.org/article/20a85ae018a445ceaab58f206287dfd62018-12-01T00:00:00Zhttps://doi.org/10.2478/amb-2018-0014https://doaj.org/toc/0324-1750Worldwide prostate cancer is the second leading cause of cancer death among men after lung cancer. MicroRNAs are non-coding, endogenous RNAs and they play a role in tumorigenesis, RNA silencing and post-transcriptional regulation of gene expression. In this study we have investigated microRNA-15a impact on transcription factors cMYB and ETS1 in prostate-carcinoma cell line PC3. The PC3 cells were transfected with a synthetic analogue and inhibitor of microRNA-15a. The study was performed using reverse transcription polymerase chain reaction and flow cytometry methods for assessing the transcript and protein levels of cMYB and ETS1, NFκB stable reporter live cell line. Statistical analysis was performed using One–way ANOVA test. We found that cMYB and ETS1 are up-regulated by the synthetic analogue of microRNA-15a at the transcription and protein level. Transfection with microRNA-15a mimic resulted in NFκB transcription factor activation as found by using the live cell reporter system. There was some opportunistic activity exhibited by the synthetic inhibitor, but less pronounced. Our data suggest that microRNA-15a could participate in prostate cancer progression by modulating cell proliferation and pro-inflammatory signaling and paves a way for further in-depth investigation of the gene regulatory networks underneath.Apostolova A.Sezer L.Hayrabedyan S.Todorova Kr.Sciendoarticlemicrorna-15aprostate cancertranscription factors cmybets1nfκbMedicineRENActa Medica Bulgarica, Vol 45, Iss 2, Pp 20-24 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	microrna-15a prostate cancer transcription factors cmyb ets1 nfκb Medicine R
spellingShingle	microrna-15a prostate cancer transcription factors cmyb ets1 nfκb Medicine R Apostolova A. Sezer L. Hayrabedyan S. Todorova Kr. The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling
description	Worldwide prostate cancer is the second leading cause of cancer death among men after lung cancer. MicroRNAs are non-coding, endogenous RNAs and they play a role in tumorigenesis, RNA silencing and post-transcriptional regulation of gene expression. In this study we have investigated microRNA-15a impact on transcription factors cMYB and ETS1 in prostate-carcinoma cell line PC3. The PC3 cells were transfected with a synthetic analogue and inhibitor of microRNA-15a. The study was performed using reverse transcription polymerase chain reaction and flow cytometry methods for assessing the transcript and protein levels of cMYB and ETS1, NFκB stable reporter live cell line. Statistical analysis was performed using One–way ANOVA test. We found that cMYB and ETS1 are up-regulated by the synthetic analogue of microRNA-15a at the transcription and protein level. Transfection with microRNA-15a mimic resulted in NFκB transcription factor activation as found by using the live cell reporter system. There was some opportunistic activity exhibited by the synthetic inhibitor, but less pronounced. Our data suggest that microRNA-15a could participate in prostate cancer progression by modulating cell proliferation and pro-inflammatory signaling and paves a way for further in-depth investigation of the gene regulatory networks underneath.
format	article
author	Apostolova A. Sezer L. Hayrabedyan S. Todorova Kr.
author_facet	Apostolova A. Sezer L. Hayrabedyan S. Todorova Kr.
author_sort	Apostolova A.
title	The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling
title_short	The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling
title_full	The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling
title_fullStr	The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling
title_full_unstemmed	The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling
title_sort	role of microrna-15a in the development of prostate cancer – effects on cell proliferation and pro-inflammatory signalling
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/20a85ae018a445ceaab58f206287dfd6
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The Role of microRNA-15A in the Development of Prostate Cancer – Effects on Cell Proliferation and Pro-Inflammatory Signalling

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