First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano

Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a widespread pathogen that could cause different illnesses in both human and animals. Presence of MRSA in animals raises concerns of their capacity to act as reservoirs, particularly in wild animals. This study aimed to charact...

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Autores principales:	Assia Mairi, Abdelaziz Touati, Alix Pantel, Alex Yahiaoui Martinez, Mourad Ahmim, Albert Sotto, Catherine Dunyach-Remy, Jean-Philippe Lavigne
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Algeria bat guano Maltese clone toxic shock syndrome toxin ST149 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/20abc3c189ab45eda63c27e2fb87de7c
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spelling	oai:doaj.org-article:20abc3c189ab45eda63c27e2fb87de7c2021-11-25T18:24:42ZFirst Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano10.3390/microorganisms91122642076-2607https://doaj.org/article/20abc3c189ab45eda63c27e2fb87de7c2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2264https://doaj.org/toc/2076-2607Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a widespread pathogen that could cause different illnesses in both human and animals. Presence of MRSA in animals raises concerns of their capacity to act as reservoirs, particularly in wild animals. This study aimed to characterize the resistance and virulence patterns of <i>S.</i> <i>aureus</i> strains isolated from bat guano in Algeria. From March to May 2016, 98 bat guano samples from Aokas’s cave (Bejaia, Algeria) were collected. Swabs were taken for microbiological studies. Isolates were identified by Vitek<sup>®</sup> MS system, and antibiotic susceptibility was determined by disk diffusion method. The clonal origin, virulence and antibiotic resistance profiles of <i>S. aureus</i> isolates were characterized by whole genome sequencing. Eleven <i>S.</i> <i>aureus</i> strains were obtained from the 98 guano samples. Seven isolates were sensitive to all antibiotics tested and four (36.3%) were resistant to penicillin G, cefoxitin and fusidic acid. The four MRSA isolates were assigned to the sequence type ST149 and related to <i>spa</i> type <i>t</i>010. These isolates harbored a SCC<i>mec</i>IV element and the fusidic acid resistance element Q6GD50 (<i>fusC</i>). They carried different virulence genes including several enterotoxins (<i>sea</i>, <i>egc</i> enterotoxin locus, <i>sec</i>, <i>sel</i>), and the toxic shock syndrome toxin (<i>tst</i>). Our results highlight that bat guano may constitute an important reservoir of MRSA strains.Assia MairiAbdelaziz TouatiAlix PantelAlex Yahiaoui MartinezMourad AhmimAlbert SottoCatherine Dunyach-RemyJean-Philippe LavigneMDPI AGarticleAlgeriabat guanoMaltese clonetoxic shock syndrome toxinST149Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2264, p 2264 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Algeria bat guano Maltese clone toxic shock syndrome toxin ST149 Biology (General) QH301-705.5
spellingShingle	Algeria bat guano Maltese clone toxic shock syndrome toxin ST149 Biology (General) QH301-705.5 Assia Mairi Abdelaziz Touati Alix Pantel Alex Yahiaoui Martinez Mourad Ahmim Albert Sotto Catherine Dunyach-Remy Jean-Philippe Lavigne First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano
description	Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a widespread pathogen that could cause different illnesses in both human and animals. Presence of MRSA in animals raises concerns of their capacity to act as reservoirs, particularly in wild animals. This study aimed to characterize the resistance and virulence patterns of <i>S.</i> <i>aureus</i> strains isolated from bat guano in Algeria. From March to May 2016, 98 bat guano samples from Aokas’s cave (Bejaia, Algeria) were collected. Swabs were taken for microbiological studies. Isolates were identified by Vitek<sup>®</sup> MS system, and antibiotic susceptibility was determined by disk diffusion method. The clonal origin, virulence and antibiotic resistance profiles of <i>S. aureus</i> isolates were characterized by whole genome sequencing. Eleven <i>S.</i> <i>aureus</i> strains were obtained from the 98 guano samples. Seven isolates were sensitive to all antibiotics tested and four (36.3%) were resistant to penicillin G, cefoxitin and fusidic acid. The four MRSA isolates were assigned to the sequence type ST149 and related to <i>spa</i> type <i>t</i>010. These isolates harbored a SCC<i>mec</i>IV element and the fusidic acid resistance element Q6GD50 (<i>fusC</i>). They carried different virulence genes including several enterotoxins (<i>sea</i>, <i>egc</i> enterotoxin locus, <i>sec</i>, <i>sel</i>), and the toxic shock syndrome toxin (<i>tst</i>). Our results highlight that bat guano may constitute an important reservoir of MRSA strains.
format	article
author	Assia Mairi Abdelaziz Touati Alix Pantel Alex Yahiaoui Martinez Mourad Ahmim Albert Sotto Catherine Dunyach-Remy Jean-Philippe Lavigne
author_facet	Assia Mairi Abdelaziz Touati Alix Pantel Alex Yahiaoui Martinez Mourad Ahmim Albert Sotto Catherine Dunyach-Remy Jean-Philippe Lavigne
author_sort	Assia Mairi
title	First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano
title_short	First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano
title_full	First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano
title_fullStr	First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano
title_full_unstemmed	First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano
title_sort	first report of cc5-mrsa-iv-scc<i>fus</i> “maltese clone” in bat guano
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/20abc3c189ab45eda63c27e2fb87de7c
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First Report of CC5-MRSA-IV-SCC<i>fus</i> “Maltese Clone” in Bat Guano

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