Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM

Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a significant negative economic impact worldwide. Aflatoxin B1 (AFB1) is a potent toxin that may induce cancer after chronic low-level exposure. This study developed a quantitative recombinant <i>Af...

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Autores principales:	Elsayed Hafez, Nourhan M. Abd El-Aziz, Amira M. G. Darwish, Mohamed G. Shehata, Amira A. Ibrahim, Asmaa M. Elframawy, Ahmed N. Badr
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aflatoxin B1 recombinant <i>AflR</i> gene VICAM HPLC I-ELISA peanut Medicine R
Acceso en línea:	https://doaj.org/article/20ad00f26f0c47afb749ba3cfd92d97e
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spelling	oai:doaj.org-article:20ad00f26f0c47afb749ba3cfd92d97e2021-11-25T19:08:31ZValidation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM10.3390/toxins131107472072-6651https://doaj.org/article/20ad00f26f0c47afb749ba3cfd92d97e2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/747https://doaj.org/toc/2072-6651Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a significant negative economic impact worldwide. Aflatoxin B1 (AFB1) is a potent toxin that may induce cancer after chronic low-level exposure. This study developed a quantitative recombinant <i>AflR</i> gene antiserum ELISA technique for aflatoxin B1 detection in contaminated food products. Aflatoxin B1 residuals from 36 food samples were analyzed with HPLC and VICAM. DNA was extracted from aflatoxin-contaminated samples and the <i>AflR</i> gene amplified using PCR. PCR products were purified and ligated into the pGEM-T vector. Recombinant plasmids were sequenced and transformed into competent <i>E. coli</i> (BL21). Molecular size and B-cell epitope prediction for the recombinant protein were assessed. The purified protein was used to induce the production of IgG antibodies in rabbits. Serum IgG was purified and labeled with alkaline phosphatase. Finally, indirect-ELISA was used to test the effectiveness of polyclonal antibodies for detection of aflatoxin B1 in food samples.Elsayed HafezNourhan M. Abd El-AzizAmira M. G. DarwishMohamed G. ShehataAmira A. IbrahimAsmaa M. ElframawyAhmed N. BadrMDPI AGarticleaflatoxin B1recombinant <i>AflR</i> geneVICAMHPLCI-ELISApeanutMedicineRENToxins, Vol 13, Iss 747, p 747 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aflatoxin B1 recombinant <i>AflR</i> gene VICAM HPLC I-ELISA peanut Medicine R
spellingShingle	aflatoxin B1 recombinant <i>AflR</i> gene VICAM HPLC I-ELISA peanut Medicine R Elsayed Hafez Nourhan M. Abd El-Aziz Amira M. G. Darwish Mohamed G. Shehata Amira A. Ibrahim Asmaa M. Elframawy Ahmed N. Badr Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM
description	Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a significant negative economic impact worldwide. Aflatoxin B1 (AFB1) is a potent toxin that may induce cancer after chronic low-level exposure. This study developed a quantitative recombinant <i>AflR</i> gene antiserum ELISA technique for aflatoxin B1 detection in contaminated food products. Aflatoxin B1 residuals from 36 food samples were analyzed with HPLC and VICAM. DNA was extracted from aflatoxin-contaminated samples and the <i>AflR</i> gene amplified using PCR. PCR products were purified and ligated into the pGEM-T vector. Recombinant plasmids were sequenced and transformed into competent <i>E. coli</i> (BL21). Molecular size and B-cell epitope prediction for the recombinant protein were assessed. The purified protein was used to induce the production of IgG antibodies in rabbits. Serum IgG was purified and labeled with alkaline phosphatase. Finally, indirect-ELISA was used to test the effectiveness of polyclonal antibodies for detection of aflatoxin B1 in food samples.
format	article
author	Elsayed Hafez Nourhan M. Abd El-Aziz Amira M. G. Darwish Mohamed G. Shehata Amira A. Ibrahim Asmaa M. Elframawy Ahmed N. Badr
author_facet	Elsayed Hafez Nourhan M. Abd El-Aziz Amira M. G. Darwish Mohamed G. Shehata Amira A. Ibrahim Asmaa M. Elframawy Ahmed N. Badr
author_sort	Elsayed Hafez
title	Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM
title_short	Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM
title_full	Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM
title_fullStr	Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM
title_full_unstemmed	Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM
title_sort	validation of new elisa technique for detection of aflatoxin b1 contamination in food products versus hplc and vicam
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/20ad00f26f0c47afb749ba3cfd92d97e
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Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM

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