Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender

Abstract Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and...

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Autores principales:	Danli Guo, Kaicheng Kang, Pu Wang, Min Li, Xianzhong Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20ae1fde47364ce5811ab3dd83a98cbd
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spelling	oai:doaj.org-article:20ae1fde47364ce5811ab3dd83a98cbd2021-12-02T18:27:50ZTranscriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender10.1038/s41598-020-63950-42045-2322https://doaj.org/article/20ae1fde47364ce5811ab3dd83a98cbd2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-63950-4https://doaj.org/toc/2045-2322Abstract Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and metabolism in local varieties. Here, de novo transcriptome analysis of inflorescence of three development stages from initial flower bud to flowering stage 50% from two lavender cultivars with contrasting EO production revealed the dynamics of 100,177 differentially expressed transcripts (DETs) in various stages of spike development within and across the cultivars. The lavender transcriptome contained 77 DETs with annotations related to terpenoid biosynthesis. The expression profiles of the 27 genes involved in the methylerythritol phosphate (MEP) pathway, 22 genes in the mevalonate (MVA) pathway, 28 genes related to monoterpene and sesquiterpene biosynthesis during inflorescence development were comprehensively characterized, and possible links between the expression changes of genes and contents of EO constituents were explored. The upregulated genes were mainly concentrated in the MEP pathway, while most genes in the MVA pathway were downregulated during flower development, and cultivars with a higher EO content presented higher expression of genes in the MEP pathway, indicating that EOs were chiefly produced through the MEP pathway. Additionally, MYB transcription factors constituted the largest number of transcripts in all samples, suggesting their potential roles in regulating EO biosynthesis. The sequences and transcriptional patterns of the transcripts will be helpful for understanding the molecular basis of lavender terpene biosynthesis.Danli GuoKaicheng KangPu WangMin LiXianzhong HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Danli Guo Kaicheng Kang Pu Wang Min Li Xianzhong Huang Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
description	Abstract Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and metabolism in local varieties. Here, de novo transcriptome analysis of inflorescence of three development stages from initial flower bud to flowering stage 50% from two lavender cultivars with contrasting EO production revealed the dynamics of 100,177 differentially expressed transcripts (DETs) in various stages of spike development within and across the cultivars. The lavender transcriptome contained 77 DETs with annotations related to terpenoid biosynthesis. The expression profiles of the 27 genes involved in the methylerythritol phosphate (MEP) pathway, 22 genes in the mevalonate (MVA) pathway, 28 genes related to monoterpene and sesquiterpene biosynthesis during inflorescence development were comprehensively characterized, and possible links between the expression changes of genes and contents of EO constituents were explored. The upregulated genes were mainly concentrated in the MEP pathway, while most genes in the MVA pathway were downregulated during flower development, and cultivars with a higher EO content presented higher expression of genes in the MEP pathway, indicating that EOs were chiefly produced through the MEP pathway. Additionally, MYB transcription factors constituted the largest number of transcripts in all samples, suggesting their potential roles in regulating EO biosynthesis. The sequences and transcriptional patterns of the transcripts will be helpful for understanding the molecular basis of lavender terpene biosynthesis.
format	article
author	Danli Guo Kaicheng Kang Pu Wang Min Li Xianzhong Huang
author_facet	Danli Guo Kaicheng Kang Pu Wang Min Li Xianzhong Huang
author_sort	Danli Guo
title	Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
title_short	Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
title_full	Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
title_fullStr	Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
title_full_unstemmed	Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
title_sort	transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/20ae1fde47364ce5811ab3dd83a98cbd
work_keys_str_mv	AT danliguo transcriptomeprofilingofspikeprovidesexpressionfeaturesofgenesrelatedtoterpenebiosynthesisinlavender AT kaichengkang transcriptomeprofilingofspikeprovidesexpressionfeaturesofgenesrelatedtoterpenebiosynthesisinlavender AT puwang transcriptomeprofilingofspikeprovidesexpressionfeaturesofgenesrelatedtoterpenebiosynthesisinlavender AT minli transcriptomeprofilingofspikeprovidesexpressionfeaturesofgenesrelatedtoterpenebiosynthesisinlavender AT xianzhonghuang transcriptomeprofilingofspikeprovidesexpressionfeaturesofgenesrelatedtoterpenebiosynthesisinlavender
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Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender

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