Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.

Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis wa...

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Autores principales:	QianQian Zhuang, Shaopeng Chen, ZhiXin Jua, Yue Yao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20b38c9b2190442091b2aae3f031a0bb
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spelling	oai:doaj.org-article:20b38c9b2190442091b2aae3f031a0bb2021-12-02T20:04:26ZJoint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.1932-620310.1371/journal.pone.0259455https://doaj.org/article/20b38c9b2190442091b2aae3f031a0bb2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259455https://doaj.org/toc/1932-6203Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.QianQian ZhuangShaopeng ChenZhiXin JuaYue YaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259455 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q QianQian Zhuang Shaopeng Chen ZhiXin Jua Yue Yao Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.
description	Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.
format	article
author	QianQian Zhuang Shaopeng Chen ZhiXin Jua Yue Yao
author_facet	QianQian Zhuang Shaopeng Chen ZhiXin Jua Yue Yao
author_sort	QianQian Zhuang
title	Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.
title_short	Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.
title_full	Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.
title_fullStr	Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.
title_full_unstemmed	Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.
title_sort	joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in hosta ventricosa.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/20b38c9b2190442091b2aae3f031a0bb
work_keys_str_mv	AT qianqianzhuang jointtranscriptomicandmetabolomicanalysisrevealsthemechanismoflowtemperaturetoleranceinhostaventricosa AT shaopengchen jointtranscriptomicandmetabolomicanalysisrevealsthemechanismoflowtemperaturetoleranceinhostaventricosa AT zhixinjua jointtranscriptomicandmetabolomicanalysisrevealsthemechanismoflowtemperaturetoleranceinhostaventricosa AT yueyao jointtranscriptomicandmetabolomicanalysisrevealsthemechanismoflowtemperaturetoleranceinhostaventricosa
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Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa.

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