Molecular Characterization and Expression Patterns of the HkSVP Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in Hemerocallis fulva

SHORT VEGETATIVE PHASE (SVP) genes are members of the well-known MADS-box gene family that play a key role in regulating vital developmental processes in plants. Hemerocallis are perennial herbs that exhibit continuous flowering development an...

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Autores principales:	Yingzhu Liu, Yike Gao, Lin Yuan, Qixiang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	floral <i>SHORT VEGETATIVE PHASE</i> MADS-box <i>Hemerocallis</i> yeast overexpression Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/20b7faffd8454602a4a76baccf316fc4
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spelling	oai:doaj.org-article:20b7faffd8454602a4a76baccf316fc42021-11-11T17:25:39ZMolecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>10.3390/ijms2221120101422-00671661-6596https://doaj.org/article/20b7faffd8454602a4a76baccf316fc42021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12010https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>SHORT VEGETATIVE PHASE</i> (<i>SVP</i>) genes are members of the well-known MADS-box gene family that play a key role in regulating vital developmental processes in plants. <i>Hemerocallis</i> are perennial herbs that exhibit continuous flowering development and have been extensively used in landscaping. However, there are few reports on the regulatory mechanism of flowering in <i>Hemerocallis</i>. To better understand the molecular basis of floral formation of <i>Hemerocallis</i>, we identified and characterized the <i>SVP</i>-like gene <i>HkSVP</i> from the <i>Hemerocallis</i> cultivar ‘Kanai Sensei’. Quantitative RT-PCR (qRT-PCR) indicated that <i>HkSVP</i> transcript was mainly expressed in the vegetative growth stage and had the highest expression in leaves, low expression in petals, pedicels and fruits, and no expression in pistils. The HkSVP encoded protein was localized in the nucleus of <i>Arabidopsis</i> protoplasts and the nucleus of onion epidermal cells. Yeast two hybrid assay revealed that HKSVP interacted with <i>Hemerocallis</i> AP1 and TFL1. Moreover, overexpression of <i>HkSVP</i> in <i>Arabidopsis</i> resulted in delayed flowering and abnormal phenotypes, including enriched trichomes, increased basal inflorescence branches and inhibition of inflorescence formation. These observations suggest that the <i>HkSVP</i> gene may play an important role in maintaining vegetative growth by participating in the construction of inflorescence structure and the development of flower organs.Yingzhu LiuYike GaoLin YuanQixiang ZhangMDPI AGarticlefloral<i>SHORT VEGETATIVE PHASE</i>MADS-box<i>Hemerocallis</i>yeastoverexpressionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12010, p 12010 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	floral <i>SHORT VEGETATIVE PHASE</i> MADS-box <i>Hemerocallis</i> yeast overexpression Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	floral <i>SHORT VEGETATIVE PHASE</i> MADS-box <i>Hemerocallis</i> yeast overexpression Biology (General) QH301-705.5 Chemistry QD1-999 Yingzhu Liu Yike Gao Lin Yuan Qixiang Zhang Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>
description	<i>SHORT VEGETATIVE PHASE</i> (<i>SVP</i>) genes are members of the well-known MADS-box gene family that play a key role in regulating vital developmental processes in plants. <i>Hemerocallis</i> are perennial herbs that exhibit continuous flowering development and have been extensively used in landscaping. However, there are few reports on the regulatory mechanism of flowering in <i>Hemerocallis</i>. To better understand the molecular basis of floral formation of <i>Hemerocallis</i>, we identified and characterized the <i>SVP</i>-like gene <i>HkSVP</i> from the <i>Hemerocallis</i> cultivar ‘Kanai Sensei’. Quantitative RT-PCR (qRT-PCR) indicated that <i>HkSVP</i> transcript was mainly expressed in the vegetative growth stage and had the highest expression in leaves, low expression in petals, pedicels and fruits, and no expression in pistils. The HkSVP encoded protein was localized in the nucleus of <i>Arabidopsis</i> protoplasts and the nucleus of onion epidermal cells. Yeast two hybrid assay revealed that HKSVP interacted with <i>Hemerocallis</i> AP1 and TFL1. Moreover, overexpression of <i>HkSVP</i> in <i>Arabidopsis</i> resulted in delayed flowering and abnormal phenotypes, including enriched trichomes, increased basal inflorescence branches and inhibition of inflorescence formation. These observations suggest that the <i>HkSVP</i> gene may play an important role in maintaining vegetative growth by participating in the construction of inflorescence structure and the development of flower organs.
format	article
author	Yingzhu Liu Yike Gao Lin Yuan Qixiang Zhang
author_facet	Yingzhu Liu Yike Gao Lin Yuan Qixiang Zhang
author_sort	Yingzhu Liu
title	Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>
title_short	Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>
title_full	Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>
title_fullStr	Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>
title_full_unstemmed	Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>
title_sort	molecular characterization and expression patterns of the <i>hksvp</i> gene reveal distinct roles in inflorescence structure and floral organ development in <i>hemerocallis fulva</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/20b7faffd8454602a4a76baccf316fc4
work_keys_str_mv	AT yingzhuliu molecularcharacterizationandexpressionpatternsoftheihksvpigenerevealdistinctrolesininflorescencestructureandfloralorgandevelopmentinihemerocallisfulvai AT yikegao molecularcharacterizationandexpressionpatternsoftheihksvpigenerevealdistinctrolesininflorescencestructureandfloralorgandevelopmentinihemerocallisfulvai AT linyuan molecularcharacterizationandexpressionpatternsoftheihksvpigenerevealdistinctrolesininflorescencestructureandfloralorgandevelopmentinihemerocallisfulvai AT qixiangzhang molecularcharacterizationandexpressionpatternsoftheihksvpigenerevealdistinctrolesininflorescencestructureandfloralorgandevelopmentinihemerocallisfulvai
_version_	1718432104575926272

Molecular Characterization and Expression Patterns of the <i>HkSVP</i> Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in <i>Hemerocallis fulva</i>

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