Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry

In this work, we demonstrate a facile, one-step synthesis of iron-based MAX compound (Fe3AlC2 nanoflakes) modified screen-printed carbon electrode (FAC/SPCE) for non-enzymatic, trace-level electrochemical detection of DNA damage biomarker 8‑hydroxy-2′-deoxyguanosine (8OHdG) in human biofluids. The S...

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Autores principales:	Lignesh Durai, Sushmee Badhulika
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Fe3AlC2 MAX phase Electrooxidation 8-hydroxy-2′-deoxyguanosine (8OHdG) Non-enzymatic Electrochemical sensor Instruments and machines QA71-90
Acceso en línea:	https://doaj.org/article/20c27531c05443d6b09a657ecc113ba1
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spelling	oai:doaj.org-article:20c27531c05443d6b09a657ecc113ba12021-11-06T04:35:54ZHighly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry2666-053910.1016/j.snr.2021.100057https://doaj.org/article/20c27531c05443d6b09a657ecc113ba12021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666053921000321https://doaj.org/toc/2666-0539In this work, we demonstrate a facile, one-step synthesis of iron-based MAX compound (Fe3AlC2 nanoflakes) modified screen-printed carbon electrode (FAC/SPCE) for non-enzymatic, trace-level electrochemical detection of DNA damage biomarker 8‑hydroxy-2′-deoxyguanosine (8OHdG) in human biofluids. The SEM micrographs and XRD reveals the flake-like morphology and crystallographic phase of the Fe3AlC2 nanoflakes. The FAC/SPCE sensor exhibits a wide linear detection range from 100 pM to 100 μM of 8OHdG with high sensitivity (i.e., 23.85 μA/nM.cm2) and a very low detection limit (LOD) of 50 pM. This outstanding performance of the FAC/SPCE sensor can be ascribed to the good electrical conductivity and electrocatalytic activity of the Fe3AlC2 nanoflakes facilitated with Al2+/Al3+ redox couple. In addition, the sensor displays an outstanding selectivity towards 8OHdG in the presence of serums albumins obtained from human (HSA) and bovine (BSA) and other interfering metabolites like glucose (Glu), dopamine (DA), ascorbic acid (AA) and uric acid (UA). The sensor is efficacious towards trace-level recognition of 8OHdG in simulated human blood serum with good recovery percentages ranging from ∼96.96% to ∼101.48%. This excellent performance of the sensor over other reported electrochemical sensors proves it as an ideal bioanalytical platform for the medical diagnosis of various deadly diseases and disorders.Lignesh DuraiSushmee BadhulikaElsevierarticleFe3AlC2MAX phaseElectrooxidation8-hydroxy-2′-deoxyguanosine (8OHdG)Non-enzymaticElectrochemical sensorInstruments and machinesQA71-90ENSensors and Actuators Reports, Vol 3, Iss , Pp 100057- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fe3AlC2 MAX phase Electrooxidation 8-hydroxy-2′-deoxyguanosine (8OHdG) Non-enzymatic Electrochemical sensor Instruments and machines QA71-90
spellingShingle	Fe3AlC2 MAX phase Electrooxidation 8-hydroxy-2′-deoxyguanosine (8OHdG) Non-enzymatic Electrochemical sensor Instruments and machines QA71-90 Lignesh Durai Sushmee Badhulika Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry
description	In this work, we demonstrate a facile, one-step synthesis of iron-based MAX compound (Fe3AlC2 nanoflakes) modified screen-printed carbon electrode (FAC/SPCE) for non-enzymatic, trace-level electrochemical detection of DNA damage biomarker 8‑hydroxy-2′-deoxyguanosine (8OHdG) in human biofluids. The SEM micrographs and XRD reveals the flake-like morphology and crystallographic phase of the Fe3AlC2 nanoflakes. The FAC/SPCE sensor exhibits a wide linear detection range from 100 pM to 100 μM of 8OHdG with high sensitivity (i.e., 23.85 μA/nM.cm2) and a very low detection limit (LOD) of 50 pM. This outstanding performance of the FAC/SPCE sensor can be ascribed to the good electrical conductivity and electrocatalytic activity of the Fe3AlC2 nanoflakes facilitated with Al2+/Al3+ redox couple. In addition, the sensor displays an outstanding selectivity towards 8OHdG in the presence of serums albumins obtained from human (HSA) and bovine (BSA) and other interfering metabolites like glucose (Glu), dopamine (DA), ascorbic acid (AA) and uric acid (UA). The sensor is efficacious towards trace-level recognition of 8OHdG in simulated human blood serum with good recovery percentages ranging from ∼96.96% to ∼101.48%. This excellent performance of the sensor over other reported electrochemical sensors proves it as an ideal bioanalytical platform for the medical diagnosis of various deadly diseases and disorders.
format	article
author	Lignesh Durai Sushmee Badhulika
author_facet	Lignesh Durai Sushmee Badhulika
author_sort	Lignesh Durai
title	Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry
title_short	Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry
title_full	Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry
title_fullStr	Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry
title_full_unstemmed	Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry
title_sort	highly selective trace level detection of dna damage biomarker using iron-based max compound modified screen-printed carbon electrode using differential pulse voltammetry
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/20c27531c05443d6b09a657ecc113ba1
work_keys_str_mv	AT ligneshdurai highlyselectivetraceleveldetectionofdnadamagebiomarkerusingironbasedmaxcompoundmodifiedscreenprintedcarbonelectrodeusingdifferentialpulsevoltammetry AT sushmeebadhulika highlyselectivetraceleveldetectionofdnadamagebiomarkerusingironbasedmaxcompoundmodifiedscreenprintedcarbonelectrodeusingdifferentialpulsevoltammetry
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Highly selective trace level detection of DNA damage biomarker using iron-based MAX compound modified screen-printed carbon electrode using differential pulse voltammetry

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