The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays

INTRODUCTION: UV-B exposure to the skin may produce ROS, decreasing collagen density and increasing MMP-1 expression. Longan leaf extract cream contains antioxidant and is expected to prevent decrease in collagen density and MMP-1 expression increase. OBJECTIVE: The research aims at administering l...

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Autores principales:	Ruri Eka Maryam Mulyaningsih, Atina Hussaana, Chodidjah Chodidjah
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2020
Materias:	longan leaf extract, collagen density, mmp-1 expression Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/20c2bf8df5054fff9d25997cb25cbc8b
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spelling	oai:doaj.org-article:20c2bf8df5054fff9d25997cb25cbc8b2021-11-25T13:16:27ZThe Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays2085-15452339-093X10.30659/sainsmed.v10i2.3341https://doaj.org/article/20c2bf8df5054fff9d25997cb25cbc8b2020-01-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/3341https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XINTRODUCTION: UV-B exposure to the skin may produce ROS, decreasing collagen density and increasing MMP-1 expression. Longan leaf extract cream contains antioxidant and is expected to prevent decrease in collagen density and MMP-1 expression increase. OBJECTIVE: The research aims at administering longan leaf extract cream to increase collagen density and decrease MMP-1 expression in BALB/c mice exposed to UV-B rays. METHODS: This experimental research employed a Post Test Only Controlled Group Design. The subjects were 24 BALB/c mice, which were randomly divided into 4 groups. The Control Group (Ctr-G)was only administered with basic cream and exposed to UV-B rays, while Groups DLS-3, DLS-6, and DLS-13were respectively administered with longan leaf extract cream at doses 3.25%, 6.5% and 13% and exposed to UV-B rays for 4 weeks at a dose of 1 MED. On day 29, a tissue excision biopsy was performed to determine the collagen density and MMP-1 expression. The collagen density preparations were made using Masson’s Trichome staining and MMP-1 preparations were made using immunohistochemical staining. RESULTS: Kruskalwallis analysis showedstatistically no significant differences in collagen density and MMP-1 expression between groups(p value> 0.05). CONCLUSION: Administration of longan leaf extract cream at doses of 3.25%, 6.5% and 13% had proven unable to increase collagen densitynor decrease the MMP-1 expression significantly after UVB exposure.Ruri Eka Maryam MulyaningsihAtina HussaanaChodidjah ChodidjahUniversitas Sultan Agung Semarangarticlelongan leaf extract, collagen density, mmp-1 expressionMedicine (General)R5-920ENSains Medika, Vol 10, Iss 2, Pp 68-74 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	longan leaf extract, collagen density, mmp-1 expression Medicine (General) R5-920
spellingShingle	longan leaf extract, collagen density, mmp-1 expression Medicine (General) R5-920 Ruri Eka Maryam Mulyaningsih Atina Hussaana Chodidjah Chodidjah The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays
description	INTRODUCTION: UV-B exposure to the skin may produce ROS, decreasing collagen density and increasing MMP-1 expression. Longan leaf extract cream contains antioxidant and is expected to prevent decrease in collagen density and MMP-1 expression increase. OBJECTIVE: The research aims at administering longan leaf extract cream to increase collagen density and decrease MMP-1 expression in BALB/c mice exposed to UV-B rays. METHODS: This experimental research employed a Post Test Only Controlled Group Design. The subjects were 24 BALB/c mice, which were randomly divided into 4 groups. The Control Group (Ctr-G)was only administered with basic cream and exposed to UV-B rays, while Groups DLS-3, DLS-6, and DLS-13were respectively administered with longan leaf extract cream at doses 3.25%, 6.5% and 13% and exposed to UV-B rays for 4 weeks at a dose of 1 MED. On day 29, a tissue excision biopsy was performed to determine the collagen density and MMP-1 expression. The collagen density preparations were made using Masson’s Trichome staining and MMP-1 preparations were made using immunohistochemical staining. RESULTS: Kruskalwallis analysis showedstatistically no significant differences in collagen density and MMP-1 expression between groups(p value> 0.05). CONCLUSION: Administration of longan leaf extract cream at doses of 3.25%, 6.5% and 13% had proven unable to increase collagen densitynor decrease the MMP-1 expression significantly after UVB exposure.
format	article
author	Ruri Eka Maryam Mulyaningsih Atina Hussaana Chodidjah Chodidjah
author_facet	Ruri Eka Maryam Mulyaningsih Atina Hussaana Chodidjah Chodidjah
author_sort	Ruri Eka Maryam Mulyaningsih
title	The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays
title_short	The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays
title_full	The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays
title_fullStr	The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays
title_full_unstemmed	The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays
title_sort	effect of administration of longan (dimocarpuslonganl steud)leaf extract cream on collagen density and mmp-1 expression in the dermis of balb/c mice exposed to uv-b rays
publisher	Universitas Sultan Agung Semarang
publishDate	2020
url	https://doaj.org/article/20c2bf8df5054fff9d25997cb25cbc8b
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The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays

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