Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms

Abstract Evidence has suggested that major peanut allergen Ara h 1 activates dendritic cells (DCs) via interaction with DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin), a C-type lectin receptor, and contributes to development of peanut allergy. Since macroph...

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Autores principales:	Maren Krause, Peter Crauwels, Frank Blanco-Pérez, Martin Globisch, Andrea Wangorsch, Thomas Henle, Jonas Lidholm, Ger van Zandbergen, Stefan Vieths, Stephan Scheurer, Masako Toda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/20c6e2aeda004d45aa57242365d95d6c
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spelling	oai:doaj.org-article:20c6e2aeda004d45aa57242365d95d6c2021-12-02T15:55:04ZHuman monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms10.1038/s41598-021-89402-12045-2322https://doaj.org/article/20c6e2aeda004d45aa57242365d95d6c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89402-1https://doaj.org/toc/2045-2322Abstract Evidence has suggested that major peanut allergen Ara h 1 activates dendritic cells (DCs) via interaction with DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin), a C-type lectin receptor, and contributes to development of peanut allergy. Since macrophages, as well as DCs, play a crucial role in innate immunity, we investigated whether natural Ara h 1 (nAra h 1) activates two different subsets of macrophages, human monocyte derived macrophage type 1 (hMDM1: pro-inflammatory model) and type 2 (hMDM2: anti-inflammatory model). hMDM1 and hMDM2 predominantly produced pro-inflammatory cytokines (IL-6 and TNF-α) and an anti-inflammatory cytokine (IL-10) in response to nAra h 1, respectively. hMDM2 took up nAra h 1 and expressed DC-SIGN at higher levels than hMDM1. However, small interfering RNA knockdown of DC-SIGN did not suppress nAra h 1 uptake and nAra h 1-mediated cytokine production in hMDM2. Inhibitors of scavenger receptor class A type I (SR-AI) suppressed the response of hMDM2, but not of hMDM1, suggesting that SR-AI is a major receptor in hMDM2 for nAra h 1 recognition and internalization. nAra h 1 appears to exert stimulatory capacity on DC and macrophages via different receptors. This study advances our understanding how a major peanut allergen interacts with innate immunity.Maren KrausePeter CrauwelsFrank Blanco-PérezMartin GlobischAndrea WangorschThomas HenleJonas LidholmGer van ZandbergenStefan ViethsStephan ScheurerMasako TodaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maren Krause Peter Crauwels Frank Blanco-Pérez Martin Globisch Andrea Wangorsch Thomas Henle Jonas Lidholm Ger van Zandbergen Stefan Vieths Stephan Scheurer Masako Toda Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
description	Abstract Evidence has suggested that major peanut allergen Ara h 1 activates dendritic cells (DCs) via interaction with DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin), a C-type lectin receptor, and contributes to development of peanut allergy. Since macrophages, as well as DCs, play a crucial role in innate immunity, we investigated whether natural Ara h 1 (nAra h 1) activates two different subsets of macrophages, human monocyte derived macrophage type 1 (hMDM1: pro-inflammatory model) and type 2 (hMDM2: anti-inflammatory model). hMDM1 and hMDM2 predominantly produced pro-inflammatory cytokines (IL-6 and TNF-α) and an anti-inflammatory cytokine (IL-10) in response to nAra h 1, respectively. hMDM2 took up nAra h 1 and expressed DC-SIGN at higher levels than hMDM1. However, small interfering RNA knockdown of DC-SIGN did not suppress nAra h 1 uptake and nAra h 1-mediated cytokine production in hMDM2. Inhibitors of scavenger receptor class A type I (SR-AI) suppressed the response of hMDM2, but not of hMDM1, suggesting that SR-AI is a major receptor in hMDM2 for nAra h 1 recognition and internalization. nAra h 1 appears to exert stimulatory capacity on DC and macrophages via different receptors. This study advances our understanding how a major peanut allergen interacts with innate immunity.
format	article
author	Maren Krause Peter Crauwels Frank Blanco-Pérez Martin Globisch Andrea Wangorsch Thomas Henle Jonas Lidholm Ger van Zandbergen Stefan Vieths Stephan Scheurer Masako Toda
author_facet	Maren Krause Peter Crauwels Frank Blanco-Pérez Martin Globisch Andrea Wangorsch Thomas Henle Jonas Lidholm Ger van Zandbergen Stefan Vieths Stephan Scheurer Masako Toda
author_sort	Maren Krause
title	Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
title_short	Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
title_full	Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
title_fullStr	Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
title_full_unstemmed	Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
title_sort	human monocyte-derived type 1 and 2 macrophages recognize ara h 1, a major peanut allergen, by different mechanisms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/20c6e2aeda004d45aa57242365d95d6c
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Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms

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