Gold nanoparticles affect the antioxidant status in selected normal human cells

Gold nanoparticles affect the antioxidant status in selected normal human cells

Noami Daems,1 Sébastien Penninckx,2 Inge Nelissen,3 Karen Van Hoecke,4 Thomas Cardinaels,4,5 Sarah Baatout,1 Carine Michiels,6 Stéphane Lucas,2 An Aerts11Radiobiology Research Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nu...

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Autores principales: Daems N, Penninckx S, Nelissen I, Van Hoecke K, Cardinaels T, Baatout S, Michiels C, Lucas S, Aerts A
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2019
Materias:
cytotoxicity
EGFR
Cetuximab
oxidative stress
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/20c81d3974c548d0b171ef40fafafd89
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spelling oai:doaj.org-article:20c81d3974c548d0b171ef40fafafd892021-12-02T06:23:55ZGold nanoparticles affect the antioxidant status in selected normal human cells1178-2013https://doaj.org/article/20c81d3974c548d0b171ef40fafafd892019-07-01T00:00:00Zhttps://www.dovepress.com/gold-nanoparticles-affect-the-antioxidant-status-in-selected-normal-hu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Noami Daems,1 Sébastien Penninckx,2 Inge Nelissen,3 Karen Van Hoecke,4 Thomas Cardinaels,4,5 Sarah Baatout,1 Carine Michiels,6 Stéphane Lucas,2 An Aerts11Radiobiology Research Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 2Research Center for the Physics of Matter and Radiation-NARILIS, University of Namur, Namur, Belgium; 3Health Department, Flemish Institute For Technological Research (VITO), Mol, Belgium; 4Radiochemistry Expert Group, Institute for Nuclear Materials Science, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 5Department of Chemistry, KU Leuven, Heverlee, Belgium; 6Unité de Recherche en Biologie Cellulaire-NARILIS, University of Namur, Namur, BelgiumPurpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453).Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization.Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo.Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stressDaems NPenninckx SNelissen IVan Hoecke KCardinaels TBaatout SMichiels CLucas SAerts ADove Medical PressarticlecytotoxicityEGFRCetuximaboxidative stressMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4991-5015 (2019)
institution DOAJ
collection DOAJ
language EN
topic cytotoxicity
EGFR
Cetuximab
oxidative stress
Medicine (General)
R5-920
spellingShingle cytotoxicity
EGFR
Cetuximab
oxidative stress
Medicine (General)
R5-920
Daems N
Penninckx S
Nelissen I
Van Hoecke K
Cardinaels T
Baatout S
Michiels C
Lucas S
Aerts A
Gold nanoparticles affect the antioxidant status in selected normal human cells
description Noami Daems,1 Sébastien Penninckx,2 Inge Nelissen,3 Karen Van Hoecke,4 Thomas Cardinaels,4,5 Sarah Baatout,1 Carine Michiels,6 Stéphane Lucas,2 An Aerts11Radiobiology Research Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 2Research Center for the Physics of Matter and Radiation-NARILIS, University of Namur, Namur, Belgium; 3Health Department, Flemish Institute For Technological Research (VITO), Mol, Belgium; 4Radiochemistry Expert Group, Institute for Nuclear Materials Science, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 5Department of Chemistry, KU Leuven, Heverlee, Belgium; 6Unité de Recherche en Biologie Cellulaire-NARILIS, University of Namur, Namur, BelgiumPurpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453).Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization.Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo.Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stress
format article
author Daems N
Penninckx S
Nelissen I
Van Hoecke K
Cardinaels T
Baatout S
Michiels C
Lucas S
Aerts A
author_facet Daems N
Penninckx S
Nelissen I
Van Hoecke K
Cardinaels T
Baatout S
Michiels C
Lucas S
Aerts A
author_sort Daems N
title Gold nanoparticles affect the antioxidant status in selected normal human cells
title_short Gold nanoparticles affect the antioxidant status in selected normal human cells
title_full Gold nanoparticles affect the antioxidant status in selected normal human cells
title_fullStr Gold nanoparticles affect the antioxidant status in selected normal human cells
title_full_unstemmed Gold nanoparticles affect the antioxidant status in selected normal human cells
title_sort gold nanoparticles affect the antioxidant status in selected normal human cells
publisher Dove Medical Press
publishDate 2019
url https://doaj.org/article/20c81d3974c548d0b171ef40fafafd89
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AT baatouts goldnanoparticlesaffecttheantioxidantstatusinselectednormalhumancells
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