Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors

Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors

Abstract The cellular-level effects of low/high frequency oscillating magnetic field on excitable cells such as neurons are well established. In contrast, the effects of a homogeneous, static magnetic field (SMF) on Central Nervous System (CNS) glial cells are less investigated. Here, we have develo...

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Autores principales: Ankshita Prasad, Daniel B. Loong Teh, Agata Blasiak, Chou Chai, Yang Wu, Payam M. Gharibani, In Hong Yang, Thang T. Phan, Kah Leong Lim, Hyunsoo Yang, Xiaogang Liu, Angelo H. All
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Science
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Acceso en línea:https://doaj.org/article/20df8abe2dd6465ca34a6b1abc058e93
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spelling oai:doaj.org-article:20df8abe2dd6465ca34a6b1abc058e932021-12-02T16:06:51ZStatic Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors10.1038/s41598-017-06331-82045-2322https://doaj.org/article/20df8abe2dd6465ca34a6b1abc058e932017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06331-8https://doaj.org/toc/2045-2322Abstract The cellular-level effects of low/high frequency oscillating magnetic field on excitable cells such as neurons are well established. In contrast, the effects of a homogeneous, static magnetic field (SMF) on Central Nervous System (CNS) glial cells are less investigated. Here, we have developed an in vitro SMF stimulation set-up to investigate the genomic effects of SMF exposure on oligodendrocyte differentiation and neurotrophic factors secretion. Human oligodendrocytes precursor cells (OPCs) were stimulated with moderate intensity SMF (0.3 T) for a period of two weeks (two hours/day). The differential gene expression of cell activity marker (c-fos), early OPC (Olig1, Olig2. Sox10), and mature oligodendrocyte markers (CNP, MBP) were quantified. The enhanced myelination capacity of the SMF stimulated oligodendrocytes was validated in a dorsal root ganglion microfluidics chamber platform. Additionally, the effects of SMF on the gene expression and secretion of neurotrophic factors- BDNF and NT3 was quantified. We also report that SMF stimulation increases the intracellular calcium influx in OPCs as well as the gene expression of L-type channel subunits-CaV1.2 and CaV1.3. Our findings emphasize the ability of glial cells such as OPCs to positively respond to moderate intensity SMF stimulation by exhibiting enhanced differentiation, functionality as well as neurotrophic factor release.Ankshita PrasadDaniel B. Loong TehAgata BlasiakChou ChaiYang WuPayam M. GharibaniIn Hong YangThang T. PhanKah Leong LimHyunsoo YangXiaogang LiuAngelo H. AllNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ankshita Prasad
Daniel B. Loong Teh
Agata Blasiak
Chou Chai
Yang Wu
Payam M. Gharibani
In Hong Yang
Thang T. Phan
Kah Leong Lim
Hyunsoo Yang
Xiaogang Liu
Angelo H. All
Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors
description Abstract The cellular-level effects of low/high frequency oscillating magnetic field on excitable cells such as neurons are well established. In contrast, the effects of a homogeneous, static magnetic field (SMF) on Central Nervous System (CNS) glial cells are less investigated. Here, we have developed an in vitro SMF stimulation set-up to investigate the genomic effects of SMF exposure on oligodendrocyte differentiation and neurotrophic factors secretion. Human oligodendrocytes precursor cells (OPCs) were stimulated with moderate intensity SMF (0.3 T) for a period of two weeks (two hours/day). The differential gene expression of cell activity marker (c-fos), early OPC (Olig1, Olig2. Sox10), and mature oligodendrocyte markers (CNP, MBP) were quantified. The enhanced myelination capacity of the SMF stimulated oligodendrocytes was validated in a dorsal root ganglion microfluidics chamber platform. Additionally, the effects of SMF on the gene expression and secretion of neurotrophic factors- BDNF and NT3 was quantified. We also report that SMF stimulation increases the intracellular calcium influx in OPCs as well as the gene expression of L-type channel subunits-CaV1.2 and CaV1.3. Our findings emphasize the ability of glial cells such as OPCs to positively respond to moderate intensity SMF stimulation by exhibiting enhanced differentiation, functionality as well as neurotrophic factor release.
format article
author Ankshita Prasad
Daniel B. Loong Teh
Agata Blasiak
Chou Chai
Yang Wu
Payam M. Gharibani
In Hong Yang
Thang T. Phan
Kah Leong Lim
Hyunsoo Yang
Xiaogang Liu
Angelo H. All
author_facet Ankshita Prasad
Daniel B. Loong Teh
Agata Blasiak
Chou Chai
Yang Wu
Payam M. Gharibani
In Hong Yang
Thang T. Phan
Kah Leong Lim
Hyunsoo Yang
Xiaogang Liu
Angelo H. All
author_sort Ankshita Prasad
title Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors
title_short Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors
title_full Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors
title_fullStr Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors
title_full_unstemmed Static Magnetic Field Stimulation Enhances Oligodendrocyte Differentiation and Secretion of Neurotrophic Factors
title_sort static magnetic field stimulation enhances oligodendrocyte differentiation and secretion of neurotrophic factors
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/20df8abe2dd6465ca34a6b1abc058e93
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